ThermoFisher Scientific invitrogen iSort Series, Руководство пользователя

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iSort
™
Automated Cell Sorter User Guide 29
2. On the Calibration & Performance Test screen, select the Sample 4 Peaks option.
3. Briefly vortex the vial of Attune ™ Performance Tracking Beads to mix, then
prepare the performance tracking (PT) bead sample by adding the following
components into a 12 × 75-mm disposable test tube.
Attune ™ Performance Tracking Beads
5% Tween
™
20 in deionized water
10X PBS
Deionized water
500 µL
2 µL
200 µL
300 µL
Total volume: 1002 µL
Note:
Attune ™ Performance Tracking Beads consist of equal concentrations of
beads of four fluorescence emission intensities.
4. Place the sample tube with the PT beads into the sample tube holder, then
move the sample tube holder to the up position.
5. Press Start Analysis to begin the analysis and follow the instructions provided
on the touchscreen. The analysis automatically stops at 100,000 events. An
event rate of between 800 and 1,200 events per second is expected.
6. After completion, the touchscreen displays the performance test result.
•
If the performance test result is Pass , press Home , then proceed with your
Analysis or Sort run (page 31).
•
If the performance test result is Fail , make sure that the bead sample was
correctly prepared, then rerun the performance test. If the test fails again,
contact Technical Support (page 139).