ThermoFisher Scientific invitrogen iSort Series, Руководство пользователя

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24 iSort
™
Automated Cell Sorter User Guide
2. After detaching the fluid lines from the containers, slightly lift and pull out the
containers from the fluidics compartment.
3. Unscrew the lid and fill the sheath fluid container with the appropriate solution.
The sheath fluid container has 2 L capacity, sufficient to process 5 mL of sample.
Do not overfill past the 2 L line on the bottle.
Note:
To reduce the risk of sheath contamination, make sure that the sheath
fluid container has been cleaned with bleach before adding sheath fluid.
Minimize air exposure to sheath when handling to minimize the chance of
contamination. We recommend that you have at least two sheath fluid
containers so that you can wash and dry one container while using the other.
4. Screw the lid back on the sheath fluid container without over-tightening it.
5. Unscrew the lid from the waste container and empty it. Treat all waste as
biohazardous.
Note:
Empty the waste container whenever you refill the sheath fluid to
prevent the overfilling of the waste container during a run.
6. Add 200 mL of full strength bleach solution into the emptied waste container to
help prevent microbial growth, then screw the lid back on without over-
tightening it.
7. Replace the containers by sliding them into the appropriate slots in the fluidics
compartment and reconnect the fluid lines.
IMPORTANT!
Do not let the filters (0.2-µm PTFE) on top of the sheath and
waste fluid containers get wet when emptying the containers. Replace the
filters if they get wet.
8. Before use, recheck the quick connectors to ensure that they are properly in
place. If the sheath fluid container is not connected, a prompt is displayed
indicating that the instrument will not be able to build pressure.
If the waste
container is not connected, fluid will leak into the interior of the instrument .
Note:
The iSort
™
Automated Cell Sorter actively monitors the fluid levels in all
fluid containers. When the sheath fluid level is low or the waste container is full,
the instrument displays the appropriate warning message. To resume the run,
follow the displayed instructions.