Thermo Scientific Surveyor RI Plus, Руководство по оборудованию

Страница: 40 / 58

3
Operation
Purging the Flowcell
28 Surveyor RI Plus Detector Hardware Manual Thermo Scientific
12. In the RI Status page, click AutoZero to zero the output signal from the RI detector.
Purging the Flowcell
You use the Purge Control feature to remove air bubbles from both compartments of the
flowcell and to fill the reference compartment of the flowcell with the mobile phase solvent.
Y To remove air bubbles and fill the reference compartment of the flowcell
1. Turn on the RI detector and the LC pump (or all the modules of your LC system).
2. Open the online Instrument window in ChromQuest or ChromQuest SI.
3. Open the RI Status page:
a. Choose Control > Instrument Status .
The Instrument Status window appears.
b. Click the Surveyor RI Plus tab.
c. Click the RI Diagnostic tab.
4. In the Purge Control area, click On .
The Purge status should display On .
5. Pump solvent through the flowcell for 10 minutes.
6. If you suspect that the flowcell contains adhering air bubbles, remove the bubbles by
turning the Purge Control On for 10 seconds and then Off for 10 seconds until all the
bubbles are removed.
Purging is complete when the purge valve can be switched from On to Off with minimal
baseline disturbance.
Note You can also check the baseline stability by starting a preview run or by
performing a baseline check. For information on using these features, see the Surveyor
Plus Getting Started with ChromQues t Guide .