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Dionex Reagent-Free Controller

Operator’s Manual

Document No. 031880

Revision 04

January 2012

Содержание Dionex RFC

Страница 1: ...Dionex Reagent Free Controller Operator s Manual Document No 031880 Revision 04 January 2012 ...

Страница 2: ... change without notice All technical information in this document is for reference purposes only System configurations and specifications in this document supersede all previous information received by the purchaser Thermo Fisher Scientific Inc makes no representations that this document is complete accurate or error free and assumes no responsibility and will not be liable for any errors omission...

Страница 3: ...Dionex RFC 30 Only 9 3 Operation 12 3 1 Turning On the Power 12 3 2 Viewing Eluent Generator and Suppressor Status 12 3 3 Dionex RFC 10 Operation 14 3 4 Dionex RFC 10 Setup 15 3 5 Dionex RFC 30 Operation 18 3 6 Dionex RFC 30 Setup 20 3 7 Gradient Operation Dionex RFC 30 Only 29 3 8 Monitoring Eluent Generator Cartridge Use Dionex RFC 30 Only 36 4 Shutting Down the Dionex RFC 37 5 Troubleshooting 3...

Страница 4: ...tions 62 B Installation 63 B 1 Dionex RFC Cable Connections 63 B 2 DX 320 DX 500 and DX 600 TTL Connections 64 B 3 DX 120 TTL Connections 66 B 4 Power Connection 69 B 5 Pump Setup 70 B 6 Dionex RFC 10 Initial Setup 72 B 7 Dionex RFC 30 Initial Setup 74 B 8 Dionex RFC 30 Eluent Generator Installation 76 C TTL Control 90 D Disconnecting a Suppressor from an Internal Power Supply 91 D 1 Disconnecting...

Страница 5: ...30 controls a Dionex AES or Dionex SRS as well as an Thermo Scientific Dionex EluGen eluent generator cartridge EGC and a Thermo Scientific Dionex CR TC Continuously Regenerated Trap Column The Dionex RFC controls these devices by supplying current to the suppressor and eluent generator and voltage to the Dionex CR TC This manual provides instructions for operating and installing the Dionex RFC 10...

Страница 6: ...ion which if not avoided may result in minor or moderate injury Also used to identify a situation or practice that may seriously damage the instrument but will not cause injury Indicates that the function or process of the instrument may be impaired Operation does not constitute a hazard Signale une situation de danger immédiat qui si elle n est pas évitée entraînera des blessures graves à mortell...

Страница 7: ... suggest how to optimize instrument performance etc Bedeutet unmittelbare Gefahr Mißachtung kann zum Tod oder schwerwiegenden Verletzungen führen Bedeutet eine mögliche Gefährdung Mißachtung kann zum Tod oder schwerwiegenden Verletzungen führen Bedeutet eine mögliche Gefährdung Mißachtung kann zu kleineren oder mittelschweren Verletzungen führen Wird auch verwendet wenn eine Situation zu schweren ...

Страница 8: ...61010 1 2001 safety CAN CSA C22 2 No 1010 1 92 A2 97 safety UL 61010C 1 2002 R8 02 safety and EN 61326 1997 A1 1998 susceptibility and immunity The symbols below appear on the Dionex RFC or on Dionex RFC labels Alternating current Protective conductor terminal earth ground Power supply is on Power supply is off Indicates a potential hazard Refer to the operator s manual for an explanation of the h...

Страница 9: ...ntrol devices The DEVICE SELECT buttons turn on or off control of individual devices Power is supplied to a device only when RFC ENABLED is on and the DEVICE SELECT LED for the device is on Figure 1 illustrates the relationship between the Device Select and RFC Enabled functions Figure 1 Summary of Dionex RFC Enabled and Device Select Functions When priming the pump turn off all devices suppressor...

Страница 10: ...ER selects the edited value and exits edit mode CANCEL cancels the value currently being edited restores the original value and exits edit mode and scroll up or down through a list of display screens see Figure 3 In edit mode the up arrow increases the value being edited and the down arrow decreases the value Press and release the button to change the value by one unit Press and hold the button to...

Страница 11: ...reens for the Dionex RFC 10 and the Dionex RFC 30 Figure 3 Dionex RFC 10 and Dionex RFC 30 Display Screens default status current voltage pump flow set concentration EGC gradient time EGC ion count EGC type suppressor type calibration Dionex RFC 30 Display Screens Dionex RFC 10 Display Screens default status current voltage suppressor current suppressor type calibration pump flow suppressor curren...

Страница 12: ... Control AC Control Button When the TTL Control position out is selected the Pump TTL input connector is enabled When the AC Control position in is selected the Dionex RFC bypasses the Pump TTL input AC control can be used with a DX 120 system Contact Thermo Fisher Scientific for details TTL Control Input Connectors The Pump input connects to a TTL output on the pump or to a DX 120 adapter cable T...

Страница 13: ...bing assembly that removes electrolysis gases created during eluent generation Eluent Generator Cartridge EGC Holder The EGC is installed in a cartridge holder which mounts on a rack on top of the Dionex RFC top cover see Figure 5 The cartridge holder also houses the high pressure degas tubing assembly and the CR TC Tubing and fittings for plumbing the cartridge degas assembly and CR TC are includ...

Страница 14: ... P N 060299 Figure 6 Eluent Generator Plumbing Schematic Eluent Out EGC INLET OUTLET Eluent In CR TC EGC DEGAS Connect To SRS AES REGEN OUT TO CR TC ELUENT OUT yellow TO CR TC ELUENT IN red EGC OUT EGC IN Connect To Pump Outlet Connect To Inject Valve Port P 2 Connect to Gas Separator Assembly Regen In Regen Out EGC VENT Send To Waste TO DEGAS ELUENT OUT TO PUMP DAMPER TO INJ VALVE IN P Eluent Out...

Страница 15: ...degas tubing assembly and on to the injection valve in the chromatography compartment or DX 120 The eluent and sample when injection occurs then flows through the chromatography components columns suppressor cell and back to the suppressor Regenerant waste from the suppressor is directed back to the CR TC and then to the EGC degas tubing where any released hydrogen or oxygen gas is removed before ...

Страница 16: ...hich the EGC concentration can increase is 100 mM in 10 seconds The default status screen see Section 3 2 displays the suppressor current and EGC concentration values as they count up In some cases the Dionex RFC is not able to ramp up to the set point at the maximum rate This occurs because the fast increase in current causes the Dionex RFC to reach its maximum voltage limits Ramping then slows t...

Страница 17: ...creen displays OFF To view the current and voltage being applied to the eluent generator and or suppressor press to display the current voltage screen see Figure 9 Figure 7 Example Default Status Screen Dionex RFC 30 Shown Figure 8 Default Status Screen Dionex RFC 10 Shown Figure 9 Current Voltage Screen Eluent Concentration EGC Type Suppressor Current Suppressor Type A blank screen indicates cont...

Страница 18: ...current is not being supplied to the device and the suppressor is off NOTE A flashing AES SRS LED indicates the AES SRS is selected but the pump flow is off Turning On the Suppressor 1 Start the pump flow This turns on the RFC ENABLED LED For a DX 320 DX 500 or DX 600 system turn on the pump flow from the module s front panel or from the chromatography software For a DX 120 system press the Pump b...

Страница 19: ...the upper limit for suppressor current After setting the type set the flow rate see page 16 and the suppressor current see page 17 Setting the Dionex RFC 10 Suppressor Type 1 Press to display the suppressor type screen see Figure 11 NOTE The NONE suppressor type indicates that a type is not selected 2 Press ENTER The SUPP TYPE entry field on the screen is highlighted see Figure 12 When priming the...

Страница 20: ...g the Dionex RFC 10 Flow Rate The flow rate screen lets you enter the flow rate used in your application 1 Press or until the pump flow rate screen see Figure 14 is displayed NOTE The flow rate range varies depending on the type of suppressor selected see Table 1 2 Press ENTER 3 Press or until the pump flow rate for your application is displayed Figure 13 Example Suppressor Type Selected Figure 14...

Страница 21: ...y that the correct suppressor type and flow rate are selected The maximum current allowed for the suppressor depends on its type and for the Atlas suppressors the flow rate see Table 2 SRS Type Flow Rate Range Dionex ASRS_2MM 0 10 1 00 mL min Dionex ASRS_4MM 0 10 3 00 mL min Dionex CSRS_2MM 0 10 0 75 mL min Dionex CSRS_4MM 0 10 3 00 mL min Dionex AAES 0 10 3 00 mL min Dionex CAES 0 10 3 00 mL min ...

Страница 22: ...eration The Dionex RFC 30 can control a suppressor an eluent generator and a CR TC The Dionex RFC 30 controls these devices by supplying current to the suppressor and eluent generator and voltage to the CR TC The front panel LEDs indicate when a device is on current or voltage is being supplied RFC ENABLED on DEVICE SELECT AES SRS EGC CR TC solid light on not flashing If either the RFC ENABLED or ...

Страница 23: ...120 front panel to turn on the pump flow and then press the SRS button to turn on the suppressor 2 If the DEVICE SELECT LED is off press the button for the device AES SRS EGC CR TC to select the device The Dionex RFC is now supplying current to the selected device s Turning Off a Device To turn off a device press the DEVICE SELECT button or turn off the pump When priming the pump turn off all devi...

Страница 24: ...With this method you disable automatic calculation and manually enter operating parameters To disable automatic calculation select one of the following suppressor types AESXCALC SRSXCALC See Setting Up the Dionex RFC 30 for Manual Current Entry on page 28 for details Table 3 summarizes the two setup methods Dionex RFC 30 Setup Method Suppressor Types Operating Limits Suppressor Current Automatic c...

Страница 25: ...r example changing from a KOH to an MSA EGC when the suppressor type is AAES When the type is NONE the device is disabled If either the EGC or the suppressor type is NONE automatic calculation is disabled Operating Ranges and Formulas Used in Automatic Calculations The suppressor type determines the maximum flow rate SRS Type Flow Rate Range Dionex ASRS_2MM 0 10 1 00 mL min Dionex ASRS_4MM 0 10 3 ...

Страница 26: ...3 00 mL min Max Conc 200 Flow CO3 0 10 1 00 mL min 1 01 3 00 mL min 12 5 mM Max Conc 12 5 Flow Dionex CSRS_2MM MSA 0 1 0 24 mL min Max Conc 20 Flow 0 25 0 75 mL min Max Conc 35 Flow Dionex CSRS_4MM MSA 0 1 3 00 mL min Max Conc 100 Flow Dionex AAES KOH 0 1 1 00 mL min 1 01 3 00 mL min 25 mM Max Conc 25 Flow CO3 0 1 1 00 mL min 1 01 3 00 mL min 12 5 mM Max Conc 12 5 Flow Dionex CAES MSA 0 10 1 00 mL...

Страница 27: ...sor current This is normally the final concentration Suppressor Type EGC Type Minimum Current Maximum Current Dionex AAES KOH Conc Flow 3 22 100 Flow 0 6 CO3 Conc Flow 3 22 2 100 Flow 0 6 Dionex ASRS_2MM Dionex ASRS_4MM KOH Conc Flow 2 474 Conc Flow 4 CO3 Conc Flow 2 474 2 Conc Flow 4 2 Dionex CAES MSA Conc Flow 3 22 100 Flow 0 6 Dionex CSRS_2MM Dionex CSRS_4MM MSA Conc Flow 2 924 Conc Flow 4 Wher...

Страница 28: ...or eluent concentration see Table 5 The suppressor current set by the Dionex RFC 30 is the lower limit of the calculated current range see Table 6 You can enter a higher suppressor current provided it is within the range Because the Dionex RFC 30 does not automatically detect the pump flow rate or the type of installed EGC or suppressor always update this information on the Dionex RFC 30 screens i...

Страница 29: ...stem with the following operating conditions AS18 column 2 mm ASRS suppressor 1 0 mL min flow rate and a KOH EGC To set the EGC type 1 From the default screen press twice to display the generator type screen Figure 16 EGC Type Screen 2 Press ENTER The GENERATOR TYPE entry field on the screen is highlighted Figure 17 Selecting the EGC Type 3 Press to select type for example KOH Figure 18 Example EG...

Страница 30: ...igure 20 Selecting the Suppressor Type 3 Press twice to select the suppressor type for example 2 mm ASRS Figure 21 Example Suppressor Type Selected 4 Press ENTER To set the flow rate 1 Press three times to display the pump flow screen Figure 22 Pump Flow Screen 2 Press ENTER 3 Press to select flow rate for example 1 00 mL min Figure 23 Example Flow Selected 4 Press ENTER Pump Flow Rate Range of Fl...

Страница 31: ...ER 3 Press to select the concentration for example 47 mM Figure 25 Example Concentration Selected 4 Press ENTER 5 Press twice to go to the suppressor current screen Figure 26 Suppressor Current Screen with Calculated Current Set Concentration limits for the selected EGC type suppressor type and flow rate Current limits for this application Calculated current recommended for this application ...

Страница 32: ...suppressor no calculation See the example below 1 Set the EGC type flow rate and eluent concentration as described in the previous example for automatic calculation 2 Then go to the suppressor type screen Figure 27 Suppressor Type Screen 3 Press ENTER The SUPP TYPE entry field is highlighted Figure 28 Selecting the Suppressor Type 4 Press and select either SRSXCALC or AESXCALC Figure 29 AESXCALC T...

Страница 33: ...L input connection to a controlling device such as the pump combination pump detector or DX 120 is used to start and stop the gradient See Section B 2 for DX 320 DX 500 and DX 600 connection instructions See Section B 3 for DX 120 instructions Figure 31 shows an example linear ramp gradient with a starting concentration of 30 mM a gradient time of 10 minutes and a final concentration of 60 mM In t...

Страница 34: ...he value in the EGC concentration screen In the example in Figure 33 the starting concentration is 30 00 mM 2 To set the gradient time press to display the EGC gradient time screen see Figure 34 NOTE When the gradient time is 0 minutes the Dionex RFC 30 is in isocratic mode A gradient time greater than 0 selects gradient mode 3 Press ENTER Figure 32 EGC Gradient Time Screen CO3 Cartridge Installed...

Страница 35: ...36 the final concentration is 60 00 mM NOTE If the Dionex RFC 30 is set up for automatic calculation see Setting Up the Dionex RFC 30 for Automatic Current Calculation on page 24 the Dionex RFC 30 uses the highest concentration entered to calculate the suppressor current This is normally the final concentration Running a Gradient 1 Verify that the Gradient TTL on the rear panel is connected to a T...

Страница 36: ...returns to the starting value Controlling the Gradient TTL For DX 320 DX 500 and DX 600 systems the Gradient TTL can be controlled from the front panel of the controlling module or from a software command if the module is controlled with software For DX 120 systems the Gradient TTL can only be controlled with software Below are examples for controlling the TTL using PeakNet 5 2 software PeakNet 6 ...

Страница 37: ...ex RFC Gradient input See the example in Figure 37 Figure 37 DX 120 Method Setup Example 4 In the Editor enter two timed events or edit existing events In the first event select the Dionex RFC Gradient output check box to turn on the Gradient TTL In the second event clear the check box to turn off the Gradient TTL ...

Страница 38: ...gram Example This example shows how to add commands for controlling the Dionex RFC Gradient TTL input to a PeakNet 6 program 1 In PeakNet 6 open the program PGM file that will be used for controlling the Dionex RFC or create a new program 2 Add two commands to the program The first command turns on the TTL or relay output that is connected to the Dionex RFC Gradient TTL input The second command tu...

Страница 39: ...hows how to edit or create a front panel Method that includes timed events for controlling the Dionex RFC Gradient TTL input In this example the Dionex RFC Gradient TTL input is connected to the TTL1 output on an IS25 pump 1 From the front panel of the pump go to the METHOD screen 2 Open a Method that you want to edit or start a new Method NOTE For a gradient pump or combination pump detector modu...

Страница 40: ...ntroller monitors eluent generator cartridge use and displays warning messages when the cartridge ion count nears depletion A warning first appear when the percentage of ions left reaches 10 Warnings are repeated at each 1 decrease in count To set up this feature When you install a new cartridge set the cartridge ion count to 100 As the cartridge is used the Dionex RFC decrements the percentage If...

Страница 41: ... Down the Dionex RFC Figure 41 EGC Ion Count Screen DX 120 Shutdown Front panel control Turn off the pump Software control Turn off the pump from the software DX 320 DX 320J Shutdown Front panel control Set flow to 0 or Turn off the pump motor Software control Write a program or method to set the flow to 0 This disables the TTL and shuts off current to the suppressor DX 500 DX 600 Shutdown Front p...

Страница 42: ... on the screen For most errors the message remains until you clear it by pressing a key Other messages remain for three seconds only and then the display reverts to the previous screen For some errors the Dionex RFC displays a relevant screen as a prompt to enter needed information see error 105 for an example Select EGC type 104 This message appears if you try to edit any parameters related to th...

Страница 43: ...C concentration gradient time final concentration or EGC type while a gradient is running To troubleshoot Wait for the gradient to complete or stop the gradient by pressing the EGC button on the front panel When you restart the concentration selected on the EGC concentration screen i e the starting gradient concentration is in effect ERR OVER VOLTAGE SUPP 120 This error appears if you press the AE...

Страница 44: ...age than the suppressor can support To troubleshoot Reduce the flow rate Rehydrate the suppressor Refer to the suppressor manual for instructions Suppressor manuals are included on the Thermo Fisher Reference Library DVD P N 053891 If the error persists replace the suppressor Refer to the suppressor manual for instructions ERR SUPPR NOT FOUND 123 This error appears when you press the AES SRS DEVIC...

Страница 45: ... see Section 6 5 ERR OVER CURRENT EGC1 140 This error may be caused by a malfunction in the EGC controller electronics To troubleshoot Call Thermo Fisher Scientific for assistance The Dionex RFC electronics components cannot be serviced by the user ERR OVER VOLTAGE EGC1 141 This error appears if you press the AES SRS DEVICE SELECT button and the Dionex RFC cannot establish a connection with the su...

Страница 46: ...100 00 mM This may indicate corrupted memory or a problem in the Moduleware the instrument control firmware installed in the Dionex RFC To troubleshoot Call Thermo Fisher Scientific for assistance The Dionex RFC electronics components and Moduleware cannot be serviced by the user ERR EGC 1 INVALID FLOW RATE 144 This error occurs when the flow rate is outside of the absolute range allowed by the Di...

Страница 47: ...he EGC To troubleshoot Check the EGC cable connections If the error persists replace the EGC see Section 6 4 ERR EGC SHORT 147 This error may be caused by a malfunction in the EGC To troubleshoot After closing the error message press the EGC DEVICE SELECT button to restart the EGC If the error reappears replace the EGC see Section 6 4 If the error persists after replacing the EGC call Thermo Fishe...

Страница 48: ...for assistance The Dionex RFC electronics components cannot be serviced by the user ERR BOARD IS BAD EGC 153 The EGC controller electronics board is malfunctioning To troubleshoot Call Thermo Fisher Scientific for assistance The Dionex RFC electronics components cannot be serviced by the user WRN NEW BOARD FOUND MUST CALIBRATE 160 This warning occurs when a new electronics board is installed To tr...

Страница 49: ...ol out position 5 2 Other Operating Problems The table below lists some routine problems that may occur when installing or operating the Dionex RFC Problem Solution I can t set the suppressor current high enough The selected suppressor type may be incorrect Current requirements vary for the two types of suppressors For the Dionex AES the maximum current allowed is 150 mA For the SRS the maximum cu...

Страница 50: ... DX 500 or DX 600 systems Check that the TTL OUTPUT USAGE is set to 0 FLOW on the PUMP OPTIONS screen see Section B 5 For systems using AC control Check that the AC Control button position in on the rear panel is selected The RFC ENABLED LED is on but pressing the DEVICE SELECT button does not turn on the device Check to make sure the device type is selected If NONE is displayed on the default scr...

Страница 51: ...lled at the factory before shipping Thereafter Moduleware updates can be downloaded using the RS 232 port on the rear panel Items Required RS 232 computer cable DB 9 male to DB 9 female included in the Dionex RFC Ship Kit Dionex RFC 10 P N 060310 Dionex RFC 30 P N 060299 Computer with a 9 pin serial port Moduleware download software and new Moduleware file The download software and Moduleware file...

Страница 52: ...start the download When complete the Dionex RFC screen displays the new Moduleware version number 8 Set the Download Operation button on the Dionex RFC rear panel to the Operation out position 6 2 Calibrating the Dionex RFC When to Calibrate Calibration is done at the factory before shipping Thereafter the Dionex RFC should be calibrated if a new electronics board is added or before a validation p...

Страница 53: ... Thermo Fisher Scientific recommends always replacing both fuses 4 Reinsert the fuse holder into its compartment and push in until the tab clicks in place 5 Reconnect the main power cord and turn on the power HIGH VOLTAGE Disconnect the main power cord from its source and also from the rear panel of the Dionex RFC HAUTE TENSION Débranchez le cordon d alimentation principal de sa source et du panne...

Страница 54: ... and then pull them apart 4 Remove the EGC VENT line see Figure 44 from the EGC by unscrewing the luer lock from the luer adapter If necessary use a wrench to hold the luer adapter in place while unscrewing the vent line Figure 44 EGC Vent Line and Luer Fitting 5 Install the luer cap P N 053981 provided with the EGC onto the luer adapter This prevents leaks from the vent opening when you turn over...

Страница 55: ...C OUT lines from the EGC inlet and outlet ports 8 Follow the instructions in the EGC manual Document No 031908 on the Thermo Scientific Reference Library DVD P N 053891 to prepare an expended EGC for disposal or to store a partially used EGC for later use 9 Turn the new EGC upside down and set it next to the holder 10 Remove the plugs from the EGC INLET and OUTLET fittings see Figure 46 Figure 46 ...

Страница 56: ...0299 Connect this coil to the EGC OUTLET port see Figure 48 Figure 48 EGC Outlet Connection for Conditioning the EGC NOTE This is a temporary connection which is used only during the EGC conditioning procedure described in the next section 13 Turn over the EGC fittings facing down Shake the EGC vigorously and tap it with the palm of your hand 10 to 15 times Check that all trapped bubbles are dislo...

Страница 57: ...ng the EGC 1 Unscrew and remove the plug from the vent opening on the side of the EGC see Figure 50 Figure 50 EGC Vent Plug 2 Direct the yellow backpressure coil from the EGC OUTLET port to a waste container Note This can be a temporary waste container such as a beaker 3 Connect the Dionex RFC 30 EG 1 cable to the EGC cable Push the two cables together and twist the locking ring to connect them 4 ...

Страница 58: ...ve the backpressure tubing from the waste container 13 Replace the vent plug in the EGC 14 Lift the EGC out of the holder turn it over and set it next to the holder 15 Disconnect the backpressure coil from the EGC OUTLET port 16 Connect the EGC OUT line from the holder to the OUTLET port see Figure 51 Figure 51 EGC Outlet Connection 17 Turn over the EGC and again shake and tap it to remove air bub...

Страница 59: ...the instructions in Section 6 4 Steps 3 through 7 to disconnect the EGC cable cap the EGC vent opening remove the EGC from the holder and disconnect the inlet and outlet tubing Set the EGC aside 3 Disconnect the CR TC cable from the Dionex RFC 30 CR TC cable Twist the locking ring to unlock the two cables and then pull them apart 4 Disconnect the TO INJ VALVE IN P line from the injection valve and...

Страница 60: ... Figure 53 Disconnecting CR TC Liquid Lines 9 Remove the CR TC from the holder 10 Remove the plugs from the ports on the new CR TC Reconnect the liquid lines that were disconnected in Step 8 to the corresponding ports on the new CR TC 11 Check that none of the lines are caught under the CR TC and then push the CR TC onto the metal stud inside the EGC holder Push down until the CR TC snaps in place...

Страница 61: ...with the palm of your hand 10 to 15 times Check that all trapped bubbles are dislodged 16 Reinstall the EGC in the holder 17 Remove the cap on the luer adapter by twisting Save the cap 18 Twist the luer lock and EGC VENT line into the luer adapter 19 Replace the holder in the rack Hydrating the CR TC 1 Locate the 10 32 to 1 4 28 coupler P N 042806 in the Dionex RFC 30 Ship Kit P N 060299 2 Connect...

Страница 62: ...d for your application Pump deionized water through the CR TC for at least 10 minutes 6 Turn off the pump and disconnect the coupler 7 After hydrating the CR TC Reconnect TO INJ VALVE IN P to port 2 on the injection valve Reconnect TO SRS AES REGEN OUT to the REGEN OUT port on the suppressor 8 Connect the cable from the CR TC to the CR TC cable from the Dionex RFC and connect the cable from the EG...

Страница 63: ... waste container 3 Follow the instructions in Section 6 4 Steps 3 through 7 to disconnect the EGC cable cap the EGC vent opening remove the EGC from the holder and disconnect the inlet and outlet tubing Set the EGC aside 4 Disconnect the CR TC cable from the Dionex RFC 30 CR TC cable Twist the locking ring to unlock the two cables and then pull them apart 5 Lift the holder out of the rack turn it ...

Страница 64: ...e through the slot on the side of the EGC holder see Figure 55 12 Turn the EGC holder right side up 13 Reconnect the EGC IN and EGC OUT lines to the EGC inlet and outlet ports 14 Turn over the EGC fittings facing down Shake the EGC vigorously and tap it with the palm of your hand 10 to 15 times Check that all trapped bubbles are dislodged 15 Reinstall the EGC in the holder 16 Remove the cap on the...

Страница 65: ... line to the clips Figure 57 EGC Vent Line and Tubing Clips 20 Route the vent line to the waste container and insert it down into the gas separator waste tube about 4 to 6 inches 21 Reconnect the CR TC and EGC cables 22 Connect the holder liquid lines to the pump suppressor injection valve and gas separator waste tube assembly 23 Turn on the power to the Dionex RFC Tubing Clips ...

Страница 66: ... rms and 0 020 mA peak to peak CR TC CV less than 200 V rms and 2 mV peak to peak Output Drift AES SRS EG CC less than 0 1 total drift for 8 hours after an initial warm up of 10 minutes CR TC CV less than 0 1 total drift for 8 hours after an initial warm up of 10 minutes Overload Protection A continuously acting constant current circuit protects the power supply for all overloads including a direc...

Страница 67: ...suppressor now if it is not already installed refer to the suppressor manual for installation instructions 2 If a suppressor is already installed and connected to an existing power supply disconnect the suppressor from the existing power supply For detailed instructions see Appendix D Briefly For DX 320 DX 500 and DX 600 systems Unplug the suppressor cable from the J3 connector on the detector ele...

Страница 68: ...m the Atlas or SRS suppressor To connect the cables push them together NOTE The EG 1 and CR TC cables are connected during the eluent generator installation procedure Section B 8 4 If you are installing a Dionex RFC in a DX 320 DX 500 or DX 600 system go on to Section B 2 5 If you are installing a Dionex RFC in a DX 120 go to Section B 3 B 2 DX 320 DX 500 and DX 600 TTL Connections NOTE For DX 120...

Страница 69: ...lected Figure 61 Dionex RFC TTL Connections NOTE The TTL Control button position out must be selected in order for the pump to use TTL 2 out to enable and disable the Dionex RFC 5 Route the TTL cable s from the Dionex RFC rear panel to the pump or combination pump detector electronics chassis 6 Connect the red and black cable to TTL 2 OUT Refer to the label inside the door of the electronics chass...

Страница 70: ...gure 63 and the DX 120 adapter cable and grommet ordered separately NOTE The DX 120 adapter cable ensures that all current to the suppressor EGC and CR TC stops if there is no flow to the pump Figure 63 DX 120 TTL Cables NOTE If TTL 2 OUT is already in use connect the gradient TTL cable to TTL 1 OUT Red and Black Twisted Wire TTL Cable P N 060611 Yellow and Black TTL Cable P N 060706 DX 120 Adapte...

Страница 71: ...Dionex RFC TTL Connections NOTE The TTL Control button position out must be selected in order for the DX 120 to control the power to the suppressor CR TC and EGC 5 Route the other end of the red and black TTL cable into the DX 120 compartment Routing cables and tubing from the Dionex RFC to inside the DX 120 Remove the grommet from the side of the DX 120 nearest the Dionex RFC and direct the tubin...

Страница 72: ...he Dionex RFC 30 for Column B in a dual column configuration connect the adapter cable to SRS B 8 Attach the adapter to the interior wall of the DX 120 using the VELCRO on the back of the adapter Figure 65 DX 120 Adapter Cable Connections 9 Using the DIP switch labeled switch 2 on Figure 62 set the SRS A current to 50 mA position 4 ON For a Column B configuration set the SRS B current to 50 mA pos...

Страница 73: ...ction B 4 B 4 Power Connection 1 Connect the power cord IEC 320 C13 from the main power receptacle on the rear panel to a grounded power source 2 Turn on the power to the Dionex RFC and to all other system modules When attaching wires to the connector plug be careful not to allow stray strands of wire to short to the adjoining position on the connector SHOCK HAZARD To avoid electrical shock use a ...

Страница 74: ...nt avec prise de terre Ne l utilisez pas et ne le branchez pas au secteur C A sans utiliser de branchement mis à la terre Le cordon d alimentation principal est utilisé comme dispositif principal de débranchement Veillez à ce que la prise de base soit située installée près du module et facilement accessible STROMSCHLAGGEFAHR Zur Vermeidung von elektrischen Schlägen ist eine geerdete Steckdose zu v...

Страница 75: ...Detail Screen NOTE When pump operation is controlled by Thermo Scientific Dionex chromatography software pressure limits can also be set from the software 3 If you are installing a Dionex RFC 10 go on to Section B 6 If you are installing a Dionex RFC 30 go on to Section B 7 100 OUTPUT 0 05uS LOCAL OVEN TEMP 0 PSI 5 00 mL MIN TTL1 TTL2 RLY1 RLY2 0 0 0 0 RANGE P POINT L 0 R 0 LIMIT DIRECT CNTRL OFFS...

Страница 76: ...you are installing a Dionex RFC 30 go on to Section B 7 B 6 Dionex RFC 10 Initial Setup NOTE See Section B 7 for Dionex RFC 30 setup Before beginning operation of the Dionex RFC 10 refer to the suppressor manual for the recommended current setting for your application DIP Switch 1 24 1 Mpa 3500 psi Pos 5 ON 2 0 Mpa 250 psi Pos 8 ON High Pressure Alarm Low Pressure Alarm Steps for initial setup of ...

Страница 77: ...gure 70 Suppressor Type Screen 2 Press ENTER The SUPP TYPE entry field is highlighted indicating that this parameter can be edited 3 Press to select the suppressor type for example ASRS_2MM Figure 71 Example Suppressor Type Selection 4 Press ENTER 5 Press three times to display the pump flow screen Figure 72 Pump Flow Screen 6 Press ENTER 7 Press to select the application flow rate for example 1 0...

Страница 78: ...l Setup Set the EGC Type 1 From the default screen press twice to display the generator type screen Figure 76 EGC Type Screen 2 Press ENTER The GENERATOR TYPE entry field on the screen is highlighted indicating that this parameter can be edited Figure 77 Selecting the EGC Type 3 Press to select the type of EGC you will be installing for example KOH Figure 78 Example EGC Type Selection 4 Press ENTE...

Страница 79: ...ou will set the application flow rate later Figure 80 1 0 mL Flow Rate Selected 4 Press ENTER Set the Initial Eluent Concentration 1 Press to display the EGC concentration screen Figure 81 EGC Concentration Screen 2 Press ENTER 3 Press to select an eluent concentration of 50 mM Note This is the concentration required for initial set up of the EGC You will set the application concentration later Fi...

Страница 80: ...eluent generator components after all plumbing connections are complete The EGC CR TC and EGC holder are shipped in separate boxes The EGC degas assembly is installed inside the EGC holder at the factory When installation is complete all components fit inside the EGC holder Figure 83 Dionex RFC 30 Components EGCRack Drip Tray EGC Holder with EGC Degas Assembly CR TC EGC Bracket Reagent Free Contro...

Страница 81: ...C ELUENT OUT yellow TO CR TC ELUENT IN red EGC OUT EGC IN Connect To Pump Outlet Connect To Inject Valve Port P 2 Connect to Gas Separator Assembly Regen In Regen Out EGC VENT Send To Waste TO DEGAS ELUENT OUT TO PUMP DAMPER TO INJ VALVE IN P Eluent Out TO CR TC REGEN OUT blue TO SRS AES REGEN OUT TO CR TC REGEN IN orange WASTE GAS SEPARATOR DEGAS ELUENT OUT TO DEGAS ELUENT IN ...

Страница 82: ...g on the CR TC mounting plate see Figure 86 This corresponds to the metal stud inside the bottom of the EGC holder see Figure 85 These are used to mount the CR TC after it is plumbed 3 Remove the plugs from the 4 ports on the CR TC see Figure 86 Figure 86 CR TC Unions Metal stud for mounting the CR TC Opening for mounting the P Port 4 Remove plugs E Electrical connection 2 Do not remove P P P P E ...

Страница 83: ...olor coded labels for the CR TC ports NOTE To help retain the fittings and ferrules on the lines remove the union from each line just before connecting the line to a port Figure 87 Plumbed CR TC 5 Check that none of the lines are caught under the CR TC and then push the CR TC onto the metal stud inside the EGC holder see Figure 88 Push down until the CR TC snaps in place Figure 88 CR TC Installed ...

Страница 84: ... CR TC 7 Turn the EGC holder right side up 8 Pull the bundled liquid lines from inside the holder to the outside Installing the EGC 1 Turn the EGC upside down fittings facing up see Figure 90 and set the EGC next to the holder Remove the plugs from the EGC INLET and OUTLET fittings see Figure 90 Figure 90 EGC Fitting Plugs CR TC Cable Fitting Plugs OUTLET INLET ...

Страница 85: ...t Connection 3 Locate the yellow 0 5 mL min 1000 psi 7 MPa pressure restrictor coil P N 053765 in the Dionex RFC 30 Ship Kit Connect this coil to the EGC OUTLET port see Figure 48 Note This is a temporary connection which is used only during the EGC conditioning procedure described on page 83 Figure 92 EGC Outlet Connection for Conditioning the EGC INLET Port EGC IN Pressure Restrictor Coil OUTLET...

Страница 86: ...he holder until it stops NOTE To avoid crimping the lines inside the holder do not twist the EGC as you turn it over Figure 93 EGC Installation 6 Connect the EG 1 cable from the Dionex RFC 30 rear panel to the EGC cable exiting the holder To connect the cables push them together and twist the locking ring 7 Fill a reservoir with deionized water and connect the reservoir to the eluent inlet line fr...

Страница 87: ...r 3 Turn on the pump 4 Press the EGC DEVICE SELECT button on the Dionex RFC 30 front panel to turn on the EGC 5 Run the EGC at the initial flow rate and concentration settings 1 mL min at 50 mM for 30 min 6 Turn off the pump 7 Remove the backpressure tubing from the waste container 8 Replace the vent plug in the EGC 9 Lift the EGC out of the holder turn it over and set it next to the holder The EG...

Страница 88: ...e 95 EGC Outlet Connection 11 Turn over the EGC and again shake and tap it to remove air bubbles 12 Reinstall the EGC in the holder Installing the EGC Holder in the Rack 1 Set the EGC rack on top of the Dionex RFC with the vertical posts oriented toward the side nearest the chromatography module or DX 120 see Figure 96 Secure the rack with the bracket Figure 96 EGC Rack EGC OUT OUTLET Bracket EGC ...

Страница 89: ... spout Install the drip tray with the spout and drain line between the vertical posts in the rack see Figure 97 Figure 97 Drip Tray with Drain Line 3 Route the drain line to a waste container 4 Slide the EGC holder with the tubing and cables between the vertical posts into the rack see Figure 98 Figure 98 EGC Installed on Rack Drain Line Drip Tray Spout ...

Страница 90: ...h the barbed luer lock onto the tubing 6 Remove the plug from the vent opening and wipe off any liquid from the EGC 7 Screw the luer adapter onto the vent opening Remove the cap on the luer adapter by twisting Save the cap NOTE To prevent leaks from the EGC always replace the luer adapter cap before moving the EGC 8 Twist the luer lock and EGC vent line into the luer adapter see Figure 99 Figure 9...

Страница 91: ...tainer Refer to the suppressor manual for installation instructions 11 Route the vent line from the EGC to the waste container and insert it down into the gas separator waste tube about 4 to 6 inches DO NOT CAP THE WASTE CONTAINER The eluent generator uses an electrolytic process that results in the production of small amounts of oxygen or hydrogen gas To ensure that the gas is not trapped in a cl...

Страница 92: ...s un contenant fermé où ils pourraient se concentrer installez un tube d évacuation du séparateur de gaz noir ID réf 045460 de 1 3 cm 0 52 po dans un réservoir d évacuation ouvert non bouché Consultez le manuel du dispositif de suppression pour obtenir des instructions d installation Orientez la conduite d évacuation de l ensemble de dégazage de l eluent generator vers le tube d évacuation Der Elu...

Страница 93: ...gure 101 Push the cables together and twist the locking ring to connect them Figure 101 EGC and CR TC Cable Connections Backpressure Note For correct operation of the eluent generator the system pressure must be maintained at between 14 and 21 MPa 2000 and 3000 psi A system pressure of 16 MPa 2300 psi is ideal Check your system pressure first and if necessary install backpressure tubing to supply ...

Страница 94: ...eluent generator Note This input is ignored if the Pump TTL is inactive Both TTL inputs are level sensitive They are inactive at 5 V and active at 0 V When the TTL is at 5 V the controlled function is off When the TTL is at 0 V the controlled function is on The Gradient TTL can also be controlled via a relay from the pump combination pump detector or detector module For relay control opening the r...

Страница 95: ...tion D 2 for DX 120 systems After disconnecting the existing power source continue installing the Dionex RFC as described in Appendix B D 1 Disconnecting the Suppressor from a DX 320 DX 500 or DX 600 System 1 Open the door of the detector or combination pump detector and locate the suppressor cable connected to J3 SRS of the SCR card in slot 2 The label on the inside of the door identifies the ele...

Страница 96: ...e see Figure 103 D 2 Disconnecting the Suppressor from a DX 120 System 1 Open the front door of the DX 120 2 Locate the SRS A Power Source cable in the main compartment see Figure 104 Figure 103 Disconnecting the SRS Power Source Cable from the SRS Cable Figure 104 The DX 120 SRS A Power Source Cable SRS A Power Source Cable ...

Страница 97: ...urce cable at the connector and unplug it from the electronics card 4 Disconnect the SRS A power source cable from the suppressor cable and remove it see Figure 105 Store the cable for future use Figure 105 Disconnecting the SRS A Power Source Cable from the Suppressor Cable ...

Страница 98: ...Dionex Reagent Free Controller 94 Doc 031880 04 1 12 ...

Страница 99: ... 8 Power cord 49 RS 232 47 CAES 17 23 Calibrating the RFC 48 Cancel button 6 Carbonate 30 Cartridge See EGC CO3 Concentration upper limits 22 Isocratic only 30 Concentration Changing 24 Upper limits 22 See also EGC RFC 30 Contacting Dionex 47 CR TC RFC 30 Control of 5 Overview 9 Turning off 19 Turning on 19 CSRS_2MM 17 23 CSRS_4MM 17 23 Current Automatic calculation of RFC 30 20 Disabling automati...

Страница 100: ...thod example 32 PeakNet 6 gradient program example 34 Starting the pump 19 DX 600 1 Disconnecting a suppressor 91 PeakNet 5 2 gradient Method example 32 PeakNet 6 gradient program example 34 Starting the pump 19 E Edit buttons 6 EGC RFC 30 Cable connection 63 Concentration 27 Concentration upper limits 22 Control of 5 Holder 9 Ion count 36 Monitoring use 36 Overview 9 Reinstalling a partially used...

Страница 101: ... Fuses 62 Replacement procedure 49 G Generator See Eluent generator RFC 30 Gradient On LED 6 32 Gradient operation RFC 30 Controlling the Gradient TTL 32 Example 29 Front panel method 35 Overview 29 PeakNet 5 2 Method example 32 PeakNet 6 program example 34 Running a gradient 31 Setting up 30 Suppressor current calculation 23 Gradient time screen RFC 30 30 Gradient TTL input RFC 30 Connector 8 Con...

Страница 102: ...umbing Eluent generator 10 Power cord 8 49 Power receptacle 8 69 Power specifications 62 Power supply Internal 91 Power switch 8 Power up 12 Pressure 89 Product warranty 47 Program PeakNet 6 gradient example 34 Pump Enabling disabling the RFC 5 Flow screen 16 TTL control 70 TTL ignored message 45 Pump flow See also Flow rate Turning on 14 Pump TTL input Connector 8 Description 90 R Ramp gradient 2...

Страница 103: ...7 Formulas for calculating current ranges 23 Maximum current allowed 17 Off 13 Setting the current RFC 10 17 Setting the current RFC 30 20 Setting the type 15 26 Suppressor current screen 27 Turning off 15 19 Turning on 14 19 Viewing the status 12 System pressure 89 T Temperature 62 Timed events 32 Trap column RFC 30 9 Troubleshooting 38 TTL connections Specifications 62 TTL Control Input connecto...

Страница 104: ...Dionex Reagent Free Controller Index 6 Doc 031880 04 1 12 ...

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Thermo Scientific Dionex RFC, Руководство оператора

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