Prism6 & Crystal Reader software User Manual
56
Empty chambers/non-injected chambers.
The Opal chips were not placed following the
recommendations provided in the IFU.
Refer to the IFU for correct placement of the Opal
chip in the Geode before starting a new run.
The Opal chip
PCR caps were not properly
placed on the chip.
If the Opal chip
PCR caps are not properly placed
on the Opal chips, a lower amount of mix (or any
amount of mix) will enter the chamber.
Refer to the IFU for instructions to properly place
the Opal chip PCR caps.
During the loading of the chips, potential
multiple droplets could have generated.
Those droplets were probably not merged
properly using the electrostatic pen.
Make sure that the electrostatic pen is working.
For more information about how to use
the electrostatic pen with the opal chips, refer to
the IFU for Opal chips.
The partitioning step did not occur.
Check that the crucial partitioning step is
included in the PCR program used.
If the problem persists, contact the Technical
Support.
The droplet was not correctly positioned in
the Opal chip well.
Ensure to follow the troubleshooting steps for
correct positioning of the droplets described in
the IFU.
