Prism6 & Crystal Reader software User Manual
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Figure 33: Detailed view of a chamber in the Crystal Reader software.
An additional feature where users can pool multiple selected chambers containing the same
sample called the “POOLING CHAMBERS” is available in the Crystal Reader software. Pooling
a set of chambers in which the same sample has been loaded allows to gain both detection
sensitivity and quantification precision. Indeed, by considering each set of pooled chambers as
one larger chamber, this pooling strategy allows to increase the analyzed volume.
If pooling (resp. unpooling) a set of chambers in which the same sample has been loaded is
required, simply select these chambers in the chip layout using “Ctrl+Click” or “Shift+Click”, and
then click on the “POOL CHAMBERS” button (resp. “UNPOOL CHAMBERS” button) in the
“Chamber Details” tab (
Fig. 14
).
All chambers pooled together will automatically share the same “Pool ID” (displayed as an
incremented number in the chip layout), as well as the same “sample name”, “chamber context”
and, for each detection channel, the same “sample type”, “reference concentration” and “dilution
factor”.
CAUTION!
The chamber pooling functionality should only be used under the assumption that pure
replicates have been loaded in the pooled chambers (e.g., the same sample has been
loaded).
Note: If pooled chambers have not been defined before scanning, it is still possible to pool the
chambers after scanning, or during data analysis using the Crystal Miner software (see Crystal
Miner software User Manual for more details).
