Prism6 & Crystal Reader software User Manual
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To find the optimal LED exposure times for new experimental conditions, the user may
create a control experiment in which only one chamber is activated, and a few scans are
run until an optimal set of exposure times is found.
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When at least one chamber of the experiment has been scanned in the experiment, the
LED exposure time should not be further edited to ensure that all the chambers of the
experiment share the same scanning parameters. If the user tries to change an exposure
value, the software will prompt to ask if the user wishes to proceed and erase the images
of the already scanned chambers.
2. Step (2 and 3) - Select the chambers to scan & Define chamber context:
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Click on the “pencil icon”
(located below the chip holder display) to modify the
selection of the chips & chambers to be scanned (by default the 12 chambers for the
Sapphire chips and 48 chambers for Opal chips are selected).
Clicking on
the “cross icon”
will deactivate the object (chamber, chip, chip holder).
Clicking on the
“plus icon”
will activate the object (chamber, chip, chip holder).
Figure 14: Click on the cross icons to deactivate objects click on the
“plus icon
”
to activate
objects.
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If an additional chip holder is required, click on the
“plus icon”
on the right side of the
chip holder display. The chip holder count will be increased.
