Prism6 & Crystal Reader software User Manual
19
Correct placement of the tray holder on the
moveable tray.
Incorrect placement of the tray holder on
the moveable tray.
Retract the moveable tray for chip scanning.
a) Press the “CLOSE TRAY” button on the Crystal Reader software homepage.
b)
Alternatively, press the “Eject” button located at the front of the Prism6
instrument (Fig 1).
Ensure that the moveable tray is fully retracted and the Prism6 instrument flap is fully closed
before chip proceeding for chip scanning.
Note: In case the tray holder, one or multiple chips or part of chips enter the Prism6 instrument
interior, turn off the Prism6 instrument immediately and contact Technical Support.
d. Instructions for chip scanning
Crystal Reader software is the operating system of the Prism6 instrument to set up the
experimental conditions for image acquisition.
e. Definition of a
Crystal Digital PCR™ experiment
An experiment is a set of samples processed during an individual Crystal
Digital PCR™
workflow on the naica® system:
o
The samples of an individual experiment were all processed on the same respective
chip type (Sapphire chips or Opal chips). Chip types cannot be mixed in a single
experiment for image acquisition with the Prism6 instrument.
o
The samples of the individual experiment were prepared using the same PCR
mastermix and the same PCR program for partitioning and amplification with the
Geode.
o
The same scanning parameters, embedded files and the experimental details will be
applied to all samples for an individual experiment during a Prism6 run, i.e.,:
•
the same exposure times (in ms) for each of the 6 LEDs;
•
the same focus value (in mm)
•
Fluorophores and targets names
•
Image analysis configuration
•
Optional:
o
Spillover compensation matrix
o
Plots configuration
o
Analyzing configuration
