Prism6 & Crystal Reader software User Manual
51
Observation
Possible cause
Recommendation
Droplets with a saturated fluorescence level.
The exposure time is too long for at least one
channel
Saturated droplets have an intensity equal to
2
16
= 65535 RFU in at least one channel. They
can be visible by clicking on “Display
Saturation Map”. Decrease the exposure time
(usually by a factor 2) set for the detection
channel where saturation is observed and re-
scan the experiment.
Shiny aggregates within droplets
The PCR mix can aggregate when it has
been heated up for too long
Optimize the PCR conditions: decreasing
denaturation times and/or the PCR master mix
concentration with a scan-rescan. It is also
possible to use the Template PCR program
"FAST-Template Stilla PCR 45 cycles.js ".
Aggregates can also occur depending on
testing conditions
Rescan the chip immediately after the first
scan.
