Operating Manual
27
1300-87076-1A Rev. 1
4. Open the detection arm and turn the pathlength selector to the appropriate position. The range 0.5 mm is
from 0.04 to 30 Abs, and 0.05 mm is from 20 to 400 Abs. All the ranges are shown on the Spex NanoSNAP
screen.
5. Add appropriate solution of at least 1 µL and tap on the
Blank
icon to establish blank data.
6. Wipe away the blank solution off the sample window and the cover window with a lint-free wipe.
7. Tab on the
Name
bar to insert the sample name (optional). The naming system is auto-numbering.
8. Add your sample of at least 1 µL and tap on the
Measure
icon for sample measurement.
9. Clean the sample window and cover on the detection arm with a lint-free wipe after the experiment. Use
deionized water, ethanol or isopropanol if needed.
10. The
Reference
(self-defined wavelength) function is optional, and can be turned on/off any time.
11. The default of the
Auto Run
function is off. If
Auto Run
is turned on, sample measurement will be
performed automatically after closing the detection arm.
Note:
1. The conversion factor range is from 0.01 to 100 cells/mL-Abs.
2. Blank is not allowed to be used for sample naming.
3. Spex NanoSNAP offers the OD 600 measuring function, but it is suggested to use cuvette photometers for
more precise data.
7.3 Calculation
The principle of OD 600 is measuring the light scatter of the particles in the sample solution. The absorbance
will differ from different spectrophotometer systems.
A modified Beer-Lambert equation is used to calculate the concentration (optional).
Equation:
c = A600 x cf/b
c
concentration of sample suspension solution in cells/mL
A600
the absorbance at 600 nm (10 mm equivalent)
cf
the cell number conversion factor, which is represented in the unit of 1 x 10
8
cells/mL
b
the pathlength in cm
Note:
The cell number conversion factor is an optional function in Spex NanoSNAP. Users can insert a self-
defined number to calculate the concentration if needed.
