Operating Manual
24
1300-87076-1A Rev. 1
13. Add your sample of at least 1 µL and select the
Measure
icon to enter sample measurement.
14. After the experiment, clean the sample window and cover on the detection arm with a lint-free wipe. Use
deionized water, ethanol or isopropanol if needed.
15. The correction function is optional and can be turned on/off any time.
16. The default of the
Auto Run
function is off. If
Auto Run
is on, sample measurement will be performed
automatically after closing the detection arm.
Note:
1. It is suggested to clean the sample window and cover window when exchanging different concentrations of
samples.
2. If the standard curve graph isn't required, you can uncheck
Generate Std. Curve
and continue to insert
data.
6.3 Calculation
For protein quantification, the concentration can be calculated by measuring the final absorbance of colorimetric
samples and standards.
The BCA method is based on the reduction of Cu2
+
by alkaline in the protein. This has a peak absorbance at
562 nm and has a baseline correction at 750 nm.
The Bradford method is based on the protein complex with Coomassie blue dye. This measures the absorbance
at 595 nm and has a baseline correction at 750 nm.
The Lowry method is based on the protein complex with copper. This has a peak absorbance at 750 nm and has
a baseline correction at 405 nm.
Note:
1. The detail protocols are described by the assay kits. Please set the protocol according to the kit instruction.
2. If users need to establish customized standard curve protocols, please refer to Section 8.2 (page 31).
