Operating Manual
34
1300-87076-1A Rev. 1
8.2.2 Protocol Operation
1. On the main menu, tap on the
More Assays
icon then the
Standard Curve Method
icon to enter protocol
selection.
2. Ensure the sample window and the cover window on the detection arm are clean.
3. Insert
Analysis Wavelength
(required).
4. Insert the
Units
(required).
5. Insert the
Correction Wavelength
if the user needs bichromatic normalization (optional).
6. Add the appropriate solution of at least 1 µL and select the
Blank
icon to establish blank data. The screen
will automatically jump to the
Standard Curve
tab.
7. Wipe away the blank solution off the sample window and the cover window.
8. Select the correct
Curve Type
according to your solution dye.
Note:
1. Linear curve type requires minimum 2 DIFFERENT concentrations to establish standard curve.
2. Interpolation curve type requires minimum 2 DIFFERENT concentrations to establish standard curve.
3.
2
nd
order polynomial curve type requires minimum 3 DIFFERENT concentrations to establish standard
curve.
9. Select the repetition frequency you need in the
Repetition
bar.
10. Tap on a cell in the
[conc.]
column to enter the concentration of your standard sample.
11. Tap on a cell in the
Abs
. column and select the
Measure
icon to establish standard data. If the repetition
frequency is higher than 1, it will automatically jump to the next cell.
12. If you want to correct the standard absorbance, select the
Measure
icon when the indicator is on the cell
you want to change.
13. You can uncheck the value if it isn't required to generate standard curve.
14. After measuring all your standard data, check
Generate Std. Curve
to establish the standard curve.
Note
: If the standard curve graph isn't required, you can uncheck
Generate Std. Curve
and continue to insert
data.
15. Add your sample of at least 1 µL and select the
Measure
icon to enter sample measurement.
16. After the experiment, clean the sample window and the cover window on the detection arm with a lint-free
wipe. Use deionized water, ethanol or isopropanol if needed.
17. The correction function is optional and can be turned on/off any time.
18. The default of the
Auto Run
function is Off. If
Auto Run
is on, sample measurement will be performed
automatically after closing the detection arm.
