Operating Manual
20
1300-87076-1A Rev. 1
6. Application: Protein Assay
This application will measure homogeneous protein absorbance value at different wavelengths, according to
different protein assay reagents. The unit of this protocol is mg/mL.
6.1 Overview of Screen Features
The screen of protein assay protocol can be separated into 3 parts: information tab bar, information report area,
and functions icons.
The information tab bar has 4 tab pages: sample data page, standard page, table page, and graph page. The
information areas show different reports on different tab pages.
6.1.1 Sample Data Tab Page
On the sample data tab page of protein assay protocol (Figure 15), the data information parts have the features
below (Table 12). This page only shows the sample data, and does not show standard data.
Figure 15. Sample Data Tab Page
Table 12. Data tab page information.
Features
Description
[conc.]
The concentration is calculated from absorbance at kit-requested wavelength. The
unit is mg/mL.
A562/A595/A750
Displays the absorbance at kit-requested wavelength. The BCA method uses 562 nm,
Bradford method uses 595 nm, and Lowry method uses 750 nm. The absorbance is
normalized to 10 mm pathlength equivalent.
Name
The sample name can be inserted here. The default is Sample.
Method
It includes protein measuring assay such as BCA method, Bradford method, and Lowry
method. The default is BCA method.
Pathlength
The light path chosen by the pathlength selector will be detected automatically and
length will be shown here. In protein assay protocol, 0.5 mm is the only available
pathlength.
Baseline Correction
The wavelength for bichromatic normalization is different from protein methods. In
the BSA method, it is 750 nm; in the Bradford method, it is 750 nm; and in the Lowry
method, it is 405 nm. The default is On. (optional)
Function icons
Sample data information
Information tabs
Insert sample name
Protein assay types
Selected pathlength
Correction on/off
(optional)
