Operating Manual
35
1300-87076-1A Rev. 1
8.2.3 Calculation
In the protocol of
Standard Curve Method
, the concentration is calculated by the absorbance values measuring
in the user-defined wavelength and the established standard curve. The standard curve can be in types of linear,
interpolation, or 2
nd
order polynomial.
Note
: The detail protocols are described by the assay design. Set the protocol according to Section 6: Protein
Assay on page 20.
8.3 UV-Vis Method
In this application, there is no concentration conversion function for the sample's absorbance. Only absorbance
measurement for samples is offered.
8.3.1 Overview
The information tab bar has 4 parts: custom setting tab page, data tab page, table tab page, and graph tab page.
In the
Custom Setting
page (Figure 31), there are 8 self-defined wavelength fields in this protocol. The setting
range is from 190 nm to 1,000 nm. At least 1 user-defined wavelength is required to activate the function icons.
Figure 31. Custom Setting Tab Page
On the
Data
tab page of UV-Vis Method protocol (Figure 32), the data information parts have the features below
(Table 20). The function icons will be activated after finishing the protocol setting on the
Custom Setting
tab
page.
Figure 32. Data Tab Page
Function icons
Information tabs
Insert self-defined
analysis wavelength
Function icons
Sample data information
Information tabs
Insert sample name
Selected pathlength
