Operating Manual
17
1300-87076-1A Rev. 1
5. Application: Protein A280
This application will measure the samples absorbance value at 280 nm, which is the peak of purified protein
absorbing UV light, to calculate the concentration. The unit of protein concentration is mg/mL. The purity of
homogeneous protein can be estimated by absorbance ratios of A260/280.
5.1 Overview of Screen Features
The screen of Protein A280 protocol can be separated into 3 parts: information tab bar, information report area
and function icons.
The information tab bar has 3 tab pages: data page, table page and graph page. The information areas show
different reports on different tab pages.
5.1.1 Data Tab Page
On the data tab page of the protocol (Figure 12), the data information parts have the features below (Table 10).
Figure 12. Data Tab Page
Table 10. Data tab page information.
Features
Description
[conc.]
The concentration is calculated from absorbance at 280 nm, and the unit is mg/mL.
A280
Displays the absorbance at 280 nm, which is normalized to a 10 mm pathlength
equivalent.
A260/280
Displays the ratio of absorbance at 260 nm and 280 nm. When the ratio of A260/280
is > 0.6, a warning icon will pop up.
Name
The sample name can be inserted here. The default is Sample.
Method
Includes protocol types such as BSA, lgG, Lysosome, 1A = 1 mg/mL, and customized
protein factor. The default is BSA.
Pathlength
The light path chosen by the pathlength selector will be detected automatically and
length will be shown here.
Baseline Correction
The wavelength for bichromatic normalization is 340 nm. This is an optional function
and the default is on.
Function icons
Information tabs
Insert sample name
Sample data information
Sample method types
Selected pathlength
Baseline correction
on/off (optional)
