Operating Manual
25
1300-87076-1A Rev. 1
7. Application: OD 600
This application will measure microbial cell samples absorbance at 600 nm which can be used for monitoring
the samples growth rate. The range of the light pathlength is shown in the unit of absorbance. The absorbance
also can be calculated to concentration with a conversion factor, which is an optional function in this protocol. If
the user inserts the conversion factors, the unit of concentration is represented in cell/mL.
7.1 Overview of Screen Features
The screen of OD 600 protocol can be separated into 3 parts: information tab bar, information report area and
function icons.
The information tab bar has 3 pages: data page, table page and graph page. The information areas show
different reports on different tab pages.
7.1.1 Data Tab Page
On the data tab page of the OD 600 protocol (Figure 19), the data information parts have the features below
(Table 14).
Figure 19. Data Tab Page
Table 14. Data tab page information.
Features
Description
[conc.]
The concentration is calculated from absorbance at 600 nm and the unit is cells/mL.
This is an optional function.
A600
Displays the absorbance at 600 nm which is normalized to a 10 mm pathlength
equivalent.
A (Ref.)
Displays the absorbance at self-defined wavelength which is normalized to a 10 mm
pathlength equivalent.
Name
The sample name can be inserted here. The default is Sample.
Factor
A self-defined conversion factor from A600 to concentration (cells/mL). This is an
optional function.
Pathlength
The light path chosen by the pathlength selector will be detected automatically and
length will be shown here.
Reference
Self-defined wavelength for bichromatic normalization. This is an optional function and
the default is Off.
Function icons
Sample data information
Information tabs
Insert sample name
Conversion factor (optional)
Selected pathlength
Reference wavelength
on/off (optional)
