INTENDED USE
/ PRINCIPLES OF OPERATION
14
MosaiQ 125 - User Manual - November 2020 - Reference n°: MMSURM.0010.02
The detail of the microarray processing is described in the following figure and table.
The microarray processing is entirely automated.
Process Step
Description
Microarray
transport
The microarray is extracted from the magazine and transported to the
microarray turntable module.
Pipetting
The pipettor module dispenses sample containing the targeted analyte
into the microarray wells.
The appropriate sample diluent is dispensed in accordance with the type
of assay. The diluents allow for dissolving of the microarray’s preservative
coating and exposure of the printed probes to the analyte.
Shaking
The microarray is shaken by the piezoelectric shaking module to reach
the required homogeneity.
Incubation
The incubator module keeps the microarrays at the required temperature
as it moves the microarrays through the different process modules. The
targeted analyte binds the printed probes depending on the specificity.
Washing
Washing 1: The appropriate wash buffer is used to remove the excess of
sample and diluent.
Washing 2: The appropriate wash buffers are used to remove the excess of
the detection reagents needed for the assay (if applicable).
Dispensing
The dispensing steps, if applicable, are used for the addition of the associated
detection and enhancement reagents resulting in a detectable reaction.
Rinsing
The system liquid is used to remove the excess of the enhancement
reagents needed for the assay (if applicable), to reveal a clean panel
ready for imaging.
Imaging
The camera module takes an image of the processed microarray.
The MosaiQ Software analyzes and interprets this image to provide the
assay result.
Disposal
The single use microarray is finally disposed of into the solid waste
container.
As shown in the following illustration, the microarray is transported from the magazine
to dedicated positions on the incubation ring corresponding to the different analytical
processing steps until imaging and final disposal.
(microarray positions during processing)
INFO
Each microarray contained inside the MosaiQ magazines is made
of two plastic chassis enclosing two panels. The panels are made
of functionalized glass onto which samples of various biological
materials are printed. Control spots are also printed to verify
that the
MosaiQ 125
has properly dispensed the MosaiQ system
reagent and/or sample on the microarray during the assay.
For more information on this topic, please refer to the MosaiQ SDS
Magazine instructions for use (IFU).
