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Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516
TM397 · Revised 12/16
www.promega.com
1.B. Specifications (continued)
Luminescence Module Specifications
Detector
Top-reading, head-on photon-counting photomultiplier tube (PMT)
Wavelength Range
350–700nm
Detection Limit
3 × 10
–21
moles of luciferase
Linear Dynamic Range
9 logs
Cross Talk
Less than 3 × 10
–5
(white, 96-well plate, Corning Cat.# 3912)
Filtered Luminescence
Filter paddle containing 5 standard filters for luminescence
including: 495nm SP, 530nm LP, 540nm SP, 600nm LP and 450nm
(8nm BP). Additional custom emission filters may also be used.
Contact Promega Technical Services.
Fluorescence Module Specifications
Detector
PIN photodiode
Light Source
Wavelength-matched LED
Read Position
Top-reading
Wavelength Selection
Filter paddle with 5 standard excitation and
emission filters. Empty filter positions are available
for custom excitation and emission wavelengths.
Contact Promega Technical Services.
Wavelengths Included
UV (Ex: 365nm, Em: 415–445nm)
Blue (Ex: 475nm, Em: 500–550nm)
Green (Ex: 520nm, Em: 580–640nm)
Red (Ex: 627nm, Em: 660–720nm)
AFC (Ex: 405nm, Em: 495–505nm)
Detection Limit
2fmol fluorescein/200µl
Linear Dynamic Range
6 logs (assay-dependent)
