Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516
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www.promega.com
TM397 · Revised 12/16
4.
From the ‘Tools’ screen, select
Injectors
. Tap the
Prime
button to initiate priming.
5.
Allow the solution to sit in the fluid path for 30 minutes before flushing the injectors.
6.
Run the
Flush
protocol nine times with 70% ethanol.
7.
Empty and clean the Waste Collection Tray, and place it on the Microplate Sample Tray.
8.
Place a container of deionized water on the bottle holder with the stainless steel inlet tube inserted to reach
the bottom of the bottle.
9.
From the ‘Tools’ screen, select
Injectors
. Tap the
Flush
button to initiate flushing.
10. Select 9 flush cycles.
11. When all flush cycles are complete, empty and clean the Waste Collection Tray, and place it on the Micro-
plate Sample Tray.
12. Flush the injector 3 times with air. A small volume of water will remain in the injector after the air purge.
5.E Cleaning the Waste Collection Tray
The Waste Collection Tray prevents liquids from splashing or spilling inside the instrument during the Prime and
Flush protocols. The maximum volume capacity of the Waste Collection Tray is approximately 50ml. Use care
when removing the Waste Collection Tray from the Microplate Sample Tray so that no liquid spills inside the
instrument.
After each prime and flush sequence, discard any fluid from the Waste Collection Tray, and rinse it with deionized
water.
5.F. Removing or Replacing Inlet and Outlet Plastic Tubing
Replacement tubing assembly is available. See Section 7.E for ordering information.
1.
Disconnect the inlet or outlet tubing from the injector pump by twisting the gray fitting counterclockwise.
2.
Discard the used injector tubing assembly.
3.
Twist the fitting of the replacement tubing into the correct port of the injector pump.
