PerkinElmer QuantaSmart 1694267, Справочное руководство

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CHAPTER 5
78 PerkinElmer Life and Analytical Sciences
Single Photon HV DAC %
The Single Photon HV DAC % field is displayed only in SPC Assays in the Count
Conditions tab in the Regions section. The high voltage supplied to the
instrument’s Photomultiplier Tube is adjustable. Lowering the high voltage
supplied to the PMT typically decreases background counts and increases
sensitivity. The default setting for this device is 70%. The optimum setting will
need to be determined empirically.
The setting is found in the Radionuclide section in the Count Conditions menu.
Defining an Assay
The process of assay definition is central to the use of the instrument software.
Assays are defined using the seven Assay Definition tabs: Assay Parameters,
Count Conditions, Count Corrections, Report Definition, Report Output, Special
Files, and Worklist. Using these seven tabs, for each assay that you define, you
will:
„
Enter descriptive information about the nature of the assay and the author of
the assay.
„
Define a sample nuclide in the sample nuclides library if one does not already
exist.
„
Link the nuclide to the assay.
„
Link standards to the assay, if necessary.
„
Specify the appropriate count conditions and count correction factors that the
instrument will use to analyze the samples.
„
Define the reports you would like the system to generate.
„
Output desired report options.
„
Define an optional worklist to designate Positive Identification numbers and
sample names that correspond to sample numbers on a printout, if desired.
The parameters defined within the context of these seven tabs can be saved and
used or edited at your discretion. All of the assay information that you define and
save becomes a functional entity only after it is associated to a protocol number.
These protocols are recognized by the instrument via a protocol flag. This device
contains an encoded, reflective metal which the instrument uses to identify the
protocol number and sample counting parameters that you have defined and
elected to use. The QuantaSmart program enables you to define an unlimited
number of assays and associate them with up to sixty protocols by enabling the
Lock Assay feature.