PerkinElmer QuantaSmart 1694267, Справочное руководство

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ADVANCED FEATURES
PerkinElmer Life and Analytical Sciences 153
Quench Indicator
Select one of the following Quench Indicating Parameters (QIPs) from the drop-
down list. These parameters are used to calculate DPM using the instrument’s
Replay feature:
„
tSIE (transformed Spectral Index of External standard) assigns a numeric
value to the quench associated with a sample. This is independent of the
quantity of radioactivity in the sample and its count rate. tSIE is the most
accurate of the quench indicator options and is typically used for low count
rate, variable quench, single label samples. You may select this Quench
Indicator only if the samples were originally counted using tSIE.
„
tSIE/AEC (transformed Spectral Index of External Standard coupled to
Automatic Efficiency Correction). tSIE assigns a numeric value to the quench
associated with a sample. As quench varies, the AEC automatically monitors
and adjusts the counting region to exclude unwanted background. This
setting is typically used for dual and triple label experiments with variable
quench samples where optimal region settings are desired. You may select
this Quench Indicator only if the samples were originally counted using tSIE.
„
SIS (Spectral Index of the Sample) assigns a numeric value to the quench
associated with a sample. The SIS is determined from the spectral shape of
the sample and is based on actual sample counts. The SIS setting is typically
used to monitor the quench level in single label, high count rate samples for
CPM assays or in single label Cherenkov counting.
Luminescence Correction
Mark this box to activate luminescence correction. During Replay, the instrument
will correct the data for counts contributed by luminescence. Note: This feature is
optional on the 2800 and 2900 series instruments.
Regions
Enter upper and lower limits for any of the three regions, A, B and C if you would
like to redefine these limits for Replay analysis of data.