Resource
Description
HiSeq and GAIIx Systems Denature and
Dilute Libraries Guide (document #
15050107)
Provides instructions for denaturing and diluting prepared libraries for a
sequencing run, and preparing a PhiX control. This step applies to most
library types.
Visit the HiSeq 2000 support page on the Illumina website for access to documentation, software downloads,
online training, and frequently asked questions.
Instrument Components
The HiSeq 2000 system comprises the instrument, monitor, instrument control computer, and accessories,
such as a keyboard, mouse, and barcode scanner. The instrument includes four main compartments: the
optics module, flow cell compartment, fluidics compartment, and reagents compartment. An illuminated
status bar indicates operating status.
Figure 1
External Components
A
Optics module—Contains optical components that enable dual surface imaging of the flow cell, imaging A,
C, G, and T at the same time using epifluorescence. The excitation laser beam passes through the objective
and the fluorescence is simultaneously collected through the same objective.
B
Flow cell compartment—Contains the vacuum-controlled flow cell stage, which holds the flow cell in place
during sequencing runs.
C
Fluidics compartment—Contains fluidics pumps that deliver reagents to the flow cell, and then to the waste
container.
D
Status bar—Uses three colors to indicate instrument status. Blue indicates that the instrument is running,
orange indicates that the instrument needs attention, and green indicates that the instrument is ready to
begin the next run.
E
Reagent compartment—Contains reagent racks that hold reagents for sequencing runs and wash solution
for instrument washes.
Flow Cell Compartment
The flow cell compartment houses the flow cell stage, the thermal stations, the vacuum system, and the
fluidics connections to each flow cell.
Document # 15011190 v03
For Research Use Only. Not for use in diagnostic procedures.
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HiSeq 2000 System Guide