Confirm Proper Flow
Checking for proper flow confirms that the flow cell and gaskets are properly installed and the manifold is
engaged.
1
Scan or enter the priming flow cell ID (barcode number) of the priming flow cell.
2
Select Position 2 from the drop-down list.
3
Confirm the following default values:
u
Volume: 125
u
Aspirate Rate: 250
u
Dispense Rate: 2000
4
Select Pump.
5
Inspect the flow cell for bubbles passing through the lanes and leaks near the manifolds.
6
If excessive bubbles are present, do as follows.
a
Check the gaskets for obstructions.
b
Reduce the aspirate rate to 100.
c
Pump another 125 µl of water to the flow cell.
d
If problems persist, remove the flow cell, repeat the cleaning steps, and reload the flow cell.
Position Tubing and Start Prime
1
Remove the 8 waste tubes for the appropriate flow cell from the waste container.
Figure 13
Position Tubing
A
Flow cell waste tubes for reagent positions 1–8
B
Condensation pump tubing
C
Paired-end priming pump tubing
2
Place each waste tube into a separate empty 15 ml tube.
Waste is collected and measured when priming is complete.
3
Select Start Prime. Monitor priming progress from the Prime screen.
4
When priming is complete, measure the waste and confirm that the volume in each tube is 1.75 ml for a
total of 14 ml.
The total is calculated as follows:
u
250 µl for each SBS position except position 2 (250 x 7 = 1.75 ml)
Document # 15011190 v03
For Research Use Only. Not for use in diagnostic procedures.
36
HiSeq 2000 System Guide