a
Check the manifold gaskets for obstructions.
b
Repeat the pump using position 6 to avoid depleting position 5.
c
Reduce the aspirate rate to 100.
d
Pump another 250 µl to the flow cell.
6
Select Next.
7
Make sure that the flow cell lever is green, and then close the flow cell compartment door.
8
Make sure that the Vacuum Engaged and Door Closed checkboxes are selected, and then select Next.
9
From the Read Barcode screen, wait for scanning to complete.
The flow cell ID is scanned to confirm a match with the flow cell ID entered at the Flow Cell Setup screen.
Scanning takes about three minutes, or seven minutes for a dual-flow cell run.
10 Select Start to begin the sequencing run.
Monitor the Run
1
Monitor run metrics from the Run Overview screen.
Figure 16
Run Overview Screen
A
Progress bar—Monitor how many cycles have been completed.
B
Fluidics graph—Expand the fluidics section to monitor chemistry steps.
C
Run Configuration—Review parameters of the current run.
D
Analysis graph—Monitor quality scores by cycle.
E
Images graph—Monitor intensities by cycle.
First Base Report
If you opted to confirm first base during run setup, the first base confirmation dialog box opens automatically
after imaging of the first cycle is complete. The run pauses at this step.
1
Review the First Base Report from the confirmation dialog box.
2
If the results are satisfactory, select Continue.
Document # 15011190 v03
For Research Use Only. Not for use in diagnostic procedures.
39
HiSeq 2000 System Guide