Figure 9
Flow Cell Positioned Against Top and Right Guide Pins
A
Top guide pin
B
Right guide pins
3
Remove your hand from the flow cell to prevent alignment drift over time.
4
Slowly move the flow cell lever to position 1 to engage the vacuum and secure the flow cell.
When the flow cell lever is blinking green, the vacuum is engaged. If the lever is not green, see
5
Wait about five seconds, and then slowly move the flow cell lever to position 2.
When the flow cell lever is solid green, the manifolds are in position and the flow cell is ready for use.
6
Make sure that the Vacuum Engaged checkbox is selected, and then select Next.
Confirm Proper Flow
Checking for proper flow confirms that the flow cell and gaskets are properly installed and the manifold is
engaged.
1
Select Position 5 from the drop-down list.
2
Confirm the following default values:
u
Volume: 250
u
Aspirate Rate: 250
u
Dispense Rate: 2000
3
Select Pump.
4
Inspect the flow cell for bubbles passing through the lanes or leaks near the manifolds.
5
If excessive bubbles are present, do as follows.
a
Check the manifold gaskets for obstructions.
b
Repeat the pump using position 6 to avoid depleting position 5.
c
Reduce the aspirate rate to 100.
d
Pump another 250 µl to the flow cell.
6
Select Next.
7
Make sure that the flow cell lever is green, and then close the flow cell compartment door.
8
Make sure that the Vacuum Engaged and Door Closed checkboxes are selected, and then select Next.
9
From the Read Barcode screen, wait for scanning to complete.
Document # 15011190 v03
For Research Use Only. Not for use in diagnostic procedures.
22
HiSeq 2000 System Guide