illumina HiSeq 2000, Системное руководство, Страница: 35 / 76

u
50-cycle SBS kit—Remove 1 tube.
u
200-cycle SBS kit—Remove 2 tubes.
6 Thaw LFN in a beaker filled with room temperature deionized water for about 20 minutes.
7 Use ICB, SB1, SB2, and SB3 directly from 2°C to 8°C storage.
Prepare ICB for Read 1 (Non-Index or Single-Index) (50-Cycle Kit)
1 Add the contents of the tube of LFN to the bottle of ICB.
2 Rinse the tube of LFN with ICB to make sure that all LFN is transferred.
3 Add the contents of the tube of EDP to the ICB and LFN solution.
4 Rinse the tube of EDP with the ICB and LFN solution to make sure that all EDP is transferred.
5 Cap the bottle containing EDP, ICB, and LFN and invert to mix.
6 Set aside on ice.
Prepare ICB for Read 1 (Non-Index or Single-Index) (200-Cycle Kit)
1 Add 47 ml ICB to an empty 250 ml bottle to result in 2 bottles of ICB.
2 Set aside the bottle containing 47 ml ICB at 2°C to 8°C for Read 2.
3 Add the contents of 2 tubes of LFN to the original bottle of ICB.
4 Rinse each LFN tube with ICB to make sure that all LFN is transferred.
5 Add 1.1 ml EDP to the ICB and LFN solution.
6 Return the unused portion of EDP to -25°C to -15°C storage.
7 Cap the bottle containing EDP, ICB, and LFN and invert to mix.
8 Set aside on ice.
Prepare ICB for Read 1 (Dual-Index)
Use the following instructions to calculate and prepare the volume of ICB required for Read 1 of a dual-
indexed run.
1 Measure the following volumes of EDP, ICB, and LFN for every 10 cycles of sequencing to be performed
in Read 1.
Reagent Volume per 10 Cycles Storage
ICB 4.57 ml 2°C to 8°C
LFN 0.6 ml -25°C to -15°C
EDP 0.11 ml -25°C to -15°C
2 Transfer the measured volume of ICB to an empty 250 ml bottle.
3 Add the measured volume of LFN to the new bottle of ICB.
4 Rinse each tube of LFN with ICB to make sure that all LFN is transferred.
5 Add the measured volume of EDP to the ICB and LFN solution.
6 Rinse the tube of EDP with the ICB and LFN solution to make sure that all EDP is transferred.
7 Cap the bottle containing EDP, ICB, and LFN and invert to mix.
Document # 15011190 v03
For Research Use Only. Not for use in diagnostic procedures.
28
HiSeq 2000 System Guide