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115 PLC 2050/2250/2500 PurifiCation SyStemS | uSer’S GuiDe
p
LC
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in
T
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LS
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115 PLC 2050/2250/2500 PurifiCation SyStemS | uSer’S GuiDe
p
LC
W
iT
H
in
T
e
G
R
A
T
e
d
e
LS
d
C
O
n
F
iG
U
R
A
Ti
O
n
Description
the low temperature, evaporative light-scattering (eLS) detector integrated in the
PLC Purification System detects compounds in the eluent of the liquid purification
chromatography system. it is capable of monitoring eluent flow rates from 100 μL/min. to
5 mL/min.
eLS detection is a nearly universal technique that can detect any analyte less volatile
than the mobile phase. unlike other modes of detection, such as uV, it is not dependent
on the absorption of radiation, nor do the absorption characteristics of the solvent affect
detection. thus, solvents which absorb uV radiation can be used. as the solvent is completely
evaporated, a gradient can be performed to optimize the separation.
eLSD is a three step process:
1. nebulization is achieved with a venture nebulizer.
2. evaporation is achieved with a heated tube. this is an adjustable parameter.
3. Light diffusion detection is measured with a photodiode.
Gas
inlet
Large
Black
nut
nebulization
Chamber
Liquid
inlet
nebulizer
Small
Black
nut
Siphon
Figure 1
ELSD Components
nebulizing
Gas tubing
Shield
holder
nebulization
Chamber
Figure 2
eLSD installed on PLC
inclined
Support
Drain tube
Shield
holder
Figure 3
Shield Installed over ELSD Components
Shield