Genevac HT-4X, Быстрое руководство по установке, Страница: 44 / 60

Genevac HT-4X Evaporation System User Manual
42
04-7415 Issue 1-16 August 2014
Example 2. Fast Lyophilisation of HPLC Fractions
Method consists of three runs linked together to form a multi stage run, with settings as
follows:
Stage 1
Ramp & remove
organic phase Stage 2
Cool
the water Stage 3
Freeze
the water Stage 4
Lyophilise
the water
Run No.
1 2 3 4
Run Data Protection
N N N N
Next Run Number (0 for none)
2 3 4 0
Run Name
Fractions Lyo 1 Fractions Lyo 2 Fractions Lyo 3 Fractions Lyo 4
Sample Holder Type
Your holder Your holder Your holder Your holder
Sample Volume (ml)
Your volume Your volume Your volume Your volume
Solvent
MeCN or MeOH &
H20
MeCN or MeOH &
H20
MeCN or MeOH &
H20
MeCN or MeOH &
H20
SampleGuard Control Temp (°C)
40 0 0 40
SampleGuard Control Channel
1 1 1 1
CoolHeat Enable Pressure (mbar)
50 50 50 50
Chamber Temperature control (°c)
preheat to 25 preheat to 0 preheat to 0 preheat to 30
Rotor Speed
High High Low Low
Heat-Off Option
Elapsed time Elapsed time Elapsed time Elapsed time
Heat-Off Elapsed Time (HH:MM)
ask for time 0:00 00:00 05:00
End-of-Run Option
Elapsed time Elapsed time Elapsed time Elapsed time
End-of-Run Elapsed Time (MM:MM)
ask for time 0:30 01:00 10:00
Auto Defrost & Drain
Y
*
N N N
Pressure Control Regime
Variable Dri-Pure Controlled pressure Full vacuum Full vacuum
Dri-Pure Start Pressure (mbar)
175 - - -
Dri-Pure End Pressure (mbar)
40 for MeCN
45 for MeOH
- - -
Dri-Pure Ramp Time (MM)_
20 - - -
Final Control Pressure (mbar)
40 for MeCN
45 for MeOH 8 - -
* Available on LyoSpeed systems only. For non-LyoSpeed systems, defrost and drain
manually before moving to next stage.