User Manual
Genevac HT-4X Evaporation System
04-7415 Issue 1-16 August
2014
37
Creating new methods
New methods can be created either
manually or using settings suggested by the
automatic programming software (refer to
Advanced Operation
–
Setting-up a
method
). Alternatively, existing methods
can copied to a new location using the
COPY TO
function in menu bar of the
SELECT RUN
screen, then optimised for a
specific application. This allows runs to be
created for development whilst the original
is preserved.
Method optimisation
Experimentation will be required when
optimising methods. To establish a
benchmark, perform an evaporation using
both SampleGuard probes and monitor the
sample drying process. The collected data
will indicate when the evaporation process
is complete, for fast lyophilisation methods,
it will also indicate when the monitored
sample has frozen and when the
lyophilisation process ends.
Use the data to optimise the method; for
example, if it can be proven that a four hour
method actually dries the samples in three
and a half hours, reduce the run time.
Several trials using different control regimes
can then be tried to see if the run time can
be reduced further. Once a fully functioning
process has been optimised, methods using
Elapsed Time
for the
Heat-Off
and
End-of-
Run
options, may employ single
SampleGuard channel temperature control.
Genevac are able to provide remote
assistance by analysing evaporation data
from a log file.
Lyophilisation
When performing lyophilisation, fill all
positions of the sample holders as samples
in partially filled holders may not freeze. A
series of trials may be required to establish
a robust lyophilisation method, your local
Genevac representative will be happy to
assist with this.