9 • DP/SP Service
Doc. 065031-04 1/08
261
9.3.2
Priming from the Control Panel
1.
Gradient pump only:
Set the channel to be primed (A, B, C, or D) to
100% on the pump Control panel in Chromeleon or Chromeleon
Xpress.
2. Under
Prime Control
on the Control panel:
a. Set the
Duration
to 300 sec.
b. Set the
Prime Rate
to 6.0 mL/min.
3. Open the priming valve on the secondary pump head (see
by turning it one-half turn counterclockwise.
NOTE If the priming valve is opened too much, air is
drawn through the valve and air bubbles can be
seen exiting the waste line.
4. To enable priming, click the
Prime
button on the Control panel.
5. Continue priming the pump until all air and previous eluents are
purged and no air bubbles can be seen exiting the waste line.
6.
Gradient pump only:
a. If all eluent lines have not been primed, select another eluent
channel and repeat the priming procedure.
b. After priming all eluent lines, click the
Prime
button to stop
priming.
c. Close the priming valve by turning it clockwise.
Do not
overtighten the priming valve.
7. Enter the
Flow
rate required for your application on the Control
panel.
8.
Gradient pump only:
Enter the required proportions of eluents A, B,
C, and D on the Control panel (under
Gradient Control
).
9. Click
Motor
on the Control panel to turn on the pump flow.
Do not use any tools to tighten the priming valve! Overtightening may
destroy the cap seal. Open or close the priming valve only when the
system pressure is down.
Содержание ICS-3000
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