98
CORN I NG ASCENT FBR PD SYSTEM
2.
Enter setpoints:
- Feed Pump Flow Setpoint (mL/min.)
- Bolus Addition Volume (mL)
- Number of Cycles (the number of Bolus Addition cycles)
- Bolus Addition OFF Duration (min.)
NOTE:
The system automatically limits Media Addition Volume to prevent sending a volume to
the MCV which exceeds the fill limit. The window with flashing red and white fields warns the
user of the error.
3.
Press
to acknowledge prompt that Bolus Addition is complete.
8.3.5 Perfusion
This phase is used to continuously add fresh media and remove media from the MCV for a specified number of cycles at user-defined
instances.
NOTE:
The user is responsible for visually monitoring the media level in the MCV to ensure levels are not unintentionally rising above or
sinking below the expected level over time.
1.
Select Auxiliary Phases.
Select Perfusion.
2.
In this example, for each cycle 80 mL of media will be removed from the MCV into the Media
Waste bottle at 70 mL/min. flow rate. Then, 80 mL of fresh media will be added to the MCV at
70 mL/min. flow rate.
Enter setpoints:
- Media Pump Addition SP (e.g., 70 mL/min.)
- Media Pump Removal SP (e.g., 70 mL/min.)
- Media Volume (e.g., 80 mL)
It is recommended that the cycle volume is at least 300 mL to compensate for dead volume
(~60 mL) in the media addition tubing.
- Number of Cycles (e.g., 10)
- Perfusion Cycle OFF Duration (which is the time delay between cycles),(e.g., 0.5 min.)
Press
to run Perfusion Phase.
This example shows the total number of cycles set at 10. Hence, the total amount of fresh
media added to the MCV will be 800 mL (10 cycles x 80 mL). The total volume of media removed
from the MCV will be 800 mL (10 cycles x 80 mL).
NOTE:
The phase will limit media volume to prevent media removal below the minimum MCV
volume.
