Biotage Isolera Four, Руководство пользователя

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411829-L, Troubleshooting April 2012 Page 6-2
6
6.4 Pump-Related Problems
NOTE
If using highly volatile (i.e. high vapor pressure) solvents such as
dichloromethane, reservoir elevation is strongly recommended. Use the
solvent tray at the top of the system. It is also highly advisable to reduce
the flow rate and, if possible, lower the ambient temperature. See “Solvent
Specifications” on page 1-19 for the vapor pressure of different solvents.
Problem Possible Causes Solutions
1. Air bubbles moving
through the
cartridge outlet
tubing in a steady
stream during
solvent delivery.
Note that saturation
of a cartridge takes
a minimum of 3 CV.
a. Little or no solvent
in the lines.
1. Replenish the solvent reservoir(s).
2. Ensure that all used solvent inlet lines are
submerged in solvent; see “Prime the System” on
page 4-33.
b. One or more solvent
inlet lines are loose.
Check fittings, loosen, and retighten. If using
flangeless tubing, see “Assembling Tubes Correctly” on
page 5-9. If using super flangeless tubing, ensure that
the ferrules are fitted onto the tube ends as described
in the instructions supplied with the ferrules.
c. One or more solvent
inlet filters are
plugged.
Sonicate or replace the solvent inlet filters; see
page 5-6.
Re-circulating the solvent is not recommended.
Particulate-free solvent is required.
If the problem persists, the pump piston seals or the
check valve seals may be worn. Please contact Biotage
1-Point Support; see contact details on page 7-1.
2. Low or inconsistent
flow delivery volume
and/or
superimposed
periodic detector
signals.
a. One or more pump
check valves are not
functioning properly.
1. Flush the pump check valves with methanol or a
similar solvent using the Check Valves Release
Wizard; see page 3-14.
2. Check for leaks in the tubing or fittings; see “Leaks”
on page 5-7.
3. If the problem persists, the pump piston seals or
the check valve seals may be worn. Please contact
Biotage 1-Point Support; see contact details on
page 7-1.
b. One or more pump
check valves are not
functioning properly
due to particulates
in the solvent.
1. Flush the pump check valves with methanol or a
similar solvent using the Check Valves Release
Wizard; see page 3-14.
2. If the problem persists, please contact Biotage
1-Point Support (see page 7-1).
c. Solvent vaporization
during refill stroke.
1. Elevate the solvent reservoir(s); use the solvent
tray.
2. If possible, lower the ambient temperature.
3. Sonicate or replace the solvent inlet filters; see
page 5-6.
4. Reduce the fill rate for the used solvents (in the
Data Administration mode). Note that Max Fill
Rate parameter can only be changed for user-
defined solvents. If using a pre-defined solvent,
make a copy of it and then change the fill rate.