411829-L, Operation
April 2012
Page 4-5
4
As a single-piston pump only delivers liquid during the dispense stroke, the maximum flow rate
when using an Isolera Prime system is equal to (Max Fill Rate x Max Dispense Rate)/(Max Fill
Rate + Max Dispense Rate). Examples: If the fill rates for the used solvents are 50 ml/min and
80 ml/min and you use a external detector with the maximum dispense rate of 100 ml/min, the
highest flow rate that can be used is (50x100)/(50+100)=33 ml/min. If the external detector is
disabled, the highest flow rate that can be used is (50x200)/(50+200)=40 ml/min.
WARNING
If small test tubes are to be used, ensure to adjust the flow rate so that no
splashing will occur when fractions are collected.
4. Select the
Rack Type
text box and select the rack type to be used; see Table 4-1 on page 4-7
for guidance. If you do not want to use the default max fraction volume for the vessel type
and/or the default dispensation order (S-pattern), select the corresponding text box and
change the setting. The software prevents you from entering a fraction volume that will
overflow the vessels.
5. Select the solvents to be used by selecting the corresponding text box in the
Solvents
field.
(They should be the solvents determined by the TLC procedure.) To show all solvents, press
Show All
. To only show the solvents connected to the system, press
Show Connected
. Note
that the solvent percentages are specified at the
Gradient
tab.
NOTE
For reversed-phase purification with methanol and water, it is strongly
recommended to premix the water with 5% of methanol and degass either
through vacuum or sonication. Degassing of protic solvent blends
decreases out-gassing of entrapped air during gradient elution, which will
impact gradient performance and flow rates.
If automatic rack allocation is enabled, all racks on the collection tray must
be of the same type. When using an Isolera Four system, the rack type to
be used is decided by the first purification that is queued up. Any
purification queued up after that must use the same rack type. To change
rack type, wait until all present runs are finished and then remove them at
the Status tab.
Figure 4-4. Parameters Tab (Isolera LS Shown)
Содержание Isolera Four
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