Biotage Isolera Four, Руководство пользователя

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411829-L, System Settings April 2012 Page 3-2
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3.2 Change Detector Settings
3.2.1 Enable or Disable the Internal Detector
1. Select the Detector tab in the right-hand panel.
2. To enable or disable the internal detector, select the Enabled text box in the UV Detector field
and select Yes or No .
3.2.2 Connect and Enable an External Detector
NOTE
The external detector and the tube assembly must be able to handle a
maximum dispense rate of 200 ml/min (when using an Isolera Prime,
Isolera One, or Isolera Four system) or 500 ml/min (when using an
Isolera LS system) and a maximum pressure of 10 bar.
As some external detectors have flow rate limitations (e.g. ELSD), the flow
stream may need to be split so that the majority of the flow is directed
through the internal detector and a proportion diverted to the external
detector, or the dispense rate (i.e. the speed at which liquid is pushed into
the tubing of the external detector) must be limited (see step 7 below).
Please contact the external detector manufacturer for flow rate
specifications.
The external ELSD-1080 detector from Biotage shall be unpacked and
installed by an authorized Biotage service engineer. Should you need to
move the detector, see the instructions in the ELSD-1080 Getting Started
Guide (P/N 412941).
To connect and enable an external detector:
1. Connect the external detector (with an analog output signal from 0 V up to a maximum of 5 V)
to the EXT UV port at the rear of the system using a male DE-9 connector:
Figure 3-2. Male DE-9 Connector
2. Connect the internal and external flow cells in series or by using a splitter, or move the internal
detector's inlet and outlet tubing (see Figure 3-3) to the external flow cell. Ensure that you use
as short extra tubing as possible. For more information, please refer to the manufacturer of the
external detector.
Tip! If the flow cells are connected in series and the pressure exceeds 10 bar
(i.e. the run is aborted), retry using a splitter.
3. Select the Detector tab in the right-hand panel.
4. Select the Enabled text box in the External Detector field and select Yes .
5. Select the Signal Range text box and enter the maximum voltage of the signal from the
external detector. The valid range is 1 mV to 5000 mV. During the run, the system will convert
0 mV to 0 mAU and the maximum voltage to 6000 mAU.
Pin Connection
1 Signal 0-5 V, referenced to pin 2
2 Ground
3-9 Not connected