Biotage Isolera Four, Руководство пользователя

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411829-L, Description and Specifications April 2012 Page 1-11
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1.2.5 Method Tab (Chemistry Mode)
At the Method tab, in the software’s Chemistry mode, you can create, open, and edit a method,
and assign it to a cartridge mounted on the system, select the rack position or positions to be used,
and start or queue up* the purification.
Figure 1-8. Method Tab (Isolera LS Shown)
There are different ways of creating a new method:
• Create a new method. If using the method wizard, it will guide you step-by-step through
the setup of a run. You can also use the TLC to Linear Gradient editor or the TLC to Step
Gradient editor (requires a Spektra license) to calculate a purification gradient and get
cartridge and sample load recommendations. After setting up the method, you can save it by
entering a unique method name.
• Base the new method on an existing method. You can either base the method on one of
the preconfigured Biotage methods or a method created and saved in the system’s database,
on a USB memory device, or in a share folder on the network. After editing, you can save the
method under a new name by entering a unique method name. The original method remains
unaltered under its original name.
• Base the new method on a previous purification (at the Results tab). After editing the
purification parameters, you can save it by entering a unique method name.
NOTE
You do not have to save your method to be able to run it.
1.2.6 Status Tab (Chemistry Mode)
At the Status tab, in the software’s Chemistry mode, you can:
• View information on a cartridge mounted on the system. Available information is: user,
sample name, status, allocated rack(s), and the estimated time when the system will have
completed the purification.
• Enable injection of a liquid sample using a syringe or the sample loading pump (the Load
Sample button) and start or queue up* a purification after a finished equilibration step.
(Only Isolera LS is equipped with the sample loading pump.)
• Change the processing order of queued purifications.*
• Start and end an isocratic segment (the Hold button).
• Enable or disable the Collect All mode and manually instruct the system to switch to a new
collection vessel.
* Purifications can be queued up when using an Isolera Four system.