411829-L, Operation
April 2012
Page 4-34
4
the (selected*) waste valve with the same solvent, we recommend that you prime with at least
60 ml when using an Isolera Prime, Isolera One, or Isolera Four system or 100 ml when using
an Isolera LS system.
9. Select the
Flowrate
text box and enter the flow rate. The maximum flow rate that can be used
depends on:
• the maximum fill rate(s) defined for the used solvent(s) in the Data Administration mode,
and
• the maximum dispense rate of the system (200 ml/min for Isolera Prime, One and Four,
and 500 ml/min for Isolera LS) or, if using an external detector, the maximum dispense
rate defined for the external detector in the System mode.
As a single-piston pump only delivers liquid during the dispense stroke, the maximum flow rate
when using an Isolera Prime system is equal to (Max Fill Rate x Max Dispense Rate)/(Max Fill
Rate + Max Dispense Rate). Examples: If the fill rates for the used solvents are 50 ml/min and
80 ml/min and you use a external detector with the maximum dispense rate of 100 ml/min, the
highest flow rate that can be used is (50x100)/(50+100)=33 ml/min. If the external detector is
disabled, the highest flow rate that can be used is (50x200)/(50+200)=40 ml/min.
10. Enter the percentage of each solvent connected to the system that shall be used in the prime.
If using an Isolera LS system and a solvent inlet line is empty or filled with air bubbles, you
should prime it separately. (If several solvents are listed and you only want to use one solvent,
enter “100” for the solvent that you want to use and enter “0” or press
Clear
for the other
solvent(s).)
11. To start priming, press
Start
.
*
When using an Isolera Four system.
Содержание Isolera Four
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