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178 Agilent InfinityLab LC Series 1260 Infinity II FLD User Manual
8
Test Functions and Calibration
Wavelength Accuracy Test
Wavelength Accuracy Test
Using the Agilent Lab Advisor
1 Set up the HPLC system and the Agilent Lab Advisor.
2 Flush the flow cell with clean bi-distilled water.
3 Turn on the FLD lamp.
4 Run the Wavelength Accuracy Test.
5 The FLD will change into the multi-excitation mode with emission
wavelength at 397 nm and scan in the range of the expected maximum of
350 nm
±
20 nm.
As result, the maxima should be found at 350 nm
±
3 nm, see Figure 62 on
page 178.
The FLD will change into the multi-emission mode with excitation
wavelength at 350 nm and scan in the range of the expected maximum of
397 nm
±
20 nm.
As result, the maxima should be found at 397 nm
±
3 nm, see Figure 62 on
page 178.
Figure 62 Excitation and Emission Spectrum (expected results)
(; QP
IL[HG IL[HG
(0 QP
(; QP
QP
(0 QP
QP