Waters Xevo TQD Operator'S, Overview And Maintenance Manual Download Page 53

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3-1

Changing the Mode of Operation

This chapter describes how to prepare the mass spectrometer for the

following modes of operation:
•

ESI (electrospray ionization)

•

ESCi (combined electrospray and atmospheric pressure chemical

ionization)

•

APCI (atmospheric pressure chemical ionization)

•

Combined Atmospheric Pressure Photoionization (APPI/APCI)

•

NanoFlow

For details about other Waters and third-party source options, refer to

the documentation supplied with the source.

Contents:

Topic

Page

Combined APPI/APCI source

3-9

NanoFlow source

3-16

Summary of Contents for Xevo TQD

Page 2: ...ng from its use Trademarks ACQUITY UPLC ACQUITY UPLC Connections INSIGHT ESCi Waters and Xevo are registered trademarks of Waters Corporation IntelliStart IonSABRE MassLynx T Wave THE SCIENCE OF WHAT...

Page 3: ...ontacting medium Information Internet The Waters Web site includes contact information for Waters locations worldwide Visit www waters com Telephone and fax From the USA or Canada phone 800 252 HPLC o...

Page 4: ...fall below 400 kPa 4 bar 58 psi during analyses that require flammable solvents Connect to the LC output with a gas fail connector to stop the LC solvent if the nitrogen supply fails Warning To avoid...

Page 5: ...rsonal injury ensure equipment placed on top of the Xevo TQD does not exceed 15kg Warning To avoid injuries from broken glass falling objects or exposure to toxic or biohazardous substances never plac...

Page 6: ...e enclosure assembly can become hot To avoid burn injuries ensure the source heater is turned off and the ion block is cool before performing maintenance on these components Warning To avoid personal...

Page 7: ...the extent of decontamination required and the level of residual contamination permissible That authority must also prescribe the method of decontamination to be used and the appropriate protection f...

Page 8: ...Definition Manufacturer Date of manufacture Part number Serial number Supply ratings Authorized representative of the European Community Confirms that a manufactured product complies with all applica...

Page 9: ...acceptable calibration methods using at least five standards to generate a standard curve The concentration range for standards must cover the entire range of quality control samples typical specimen...

Page 10: ...recipitation liquid liquid extraction LLE or solid phase extraction SPE to remove matrix interferences Whenever possible verify method accuracy and precision using matrix matched calibrators and QC sa...

Page 11: ...nductively coupled radio frequency energy that is necessary for the internal functioning of the equipment Class A products are suitable for use in commercial that is nonresidential locations and can b...

Page 12: ...xii...

Page 13: ...classification xi ISM Classification ISM Group 1 Class A 1 xi EC authorized representative xi 1 Specifications and Operating Modes 1 1 Uses and compatibility 1 2 ACQUITY H Class and nanoACQUITY Xevo T...

Page 14: ...17 Constant neutral loss mode 1 19 Sample inlet 1 19 Leak sensors 1 20 Vacuum system 1 20 Rear panel connections 1 21 2 Preparing for Operation 2 1 Starting the mass spectrometer 2 2 Verifying the ins...

Page 15: ...e 3 13 Removing the IonSABRE II probe and APPI APCI source enclosure 3 15 NanoFlow source 3 16 Installing the NanoFlow source 3 17 Fitting a borosilicate glass capillary nanovial 3 19 Positioning the...

Page 16: ...urce components 4 27 Cleaning the sampling cone assembly 4 28 Removing the sampling cone assembly from the source 4 28 Disassembling the sampling cone assembly 4 30 Cleaning the sample cone and cone g...

Page 17: ...be tip and gasket 4 67 Replacing the ESI probe sample capillary 4 69 Removing the existing capillary 4 69 Installing the new capillary 4 73 Cleaning the IonSABRE II probe tip 4 77 Replacing the IonSAB...

Page 18: ...115 Replacing the instrument s fuses 4 117 A Safety Advisories A 1 Warning symbols A 2 Task specific hazard warnings A 2 Specific warnings A 3 Caution symbol A 5 Warnings that apply to all Waters inst...

Page 19: ...hernet cables B 19 I O signal connectors B 20 Signal connections B 22 Connecting to the power supply B 25 C Materials of Construction and Compliant Solvents C 1 Preventing contamination C 2 Items expo...

Page 20: ...xx Table of Contents...

Page 21: ...onnections for gas and plumbing Contents Topic Page Uses and compatibility 1 2 Ionization techniques and source probes 1 9 IntelliStart Fluidics system 1 11 Ion optics 1 13 MS operating modes 1 14 MS...

Page 22: ...ion combined electrospray ionization and atmospheric pressure chemical ionization ESI APCI ESCi source Requirement Dedicated APCI operation requires an additional probe IonSABRE II Optional dual mode...

Page 23: ...Uses and compatibility 1 3 Xevo TQD TP03406...

Page 24: ...1 4 Specifications and Operating Modes Xevo TQD with visor up TP03407...

Page 25: ...TY H Class and nanoACQUITY Xevo TQD UPLC MS systems The Waters Xevo TQD is compatible with the ACQUITY UPLC H Class and nanoACQUITY UPLC systems If you are not using either system refer to the documen...

Page 26: ...rolling Contamination in UPLC MS and HPLC MS Systems part number 715001307 You can find the documents on http www waters com click Services and Support Support Xevo TQD with ACQUITY UPLC system Xevo T...

Page 27: ...C System Operator s Guide or Controlling Contamination in LC MS Systems part number 715001307 You can find these documents on http www waters com click Services and Support Support Non ACQUITY devices...

Page 28: ...ormation on using MassLynx software OpenLynx TM and TargetLynx TM application managers are included as standard software Instrument Console software The Instrument Console is a software application wi...

Page 29: ...nt on the mass spectrometer In ESCi the standard ESI probe is used in conjunction with a corona pin to allow alternating acquisition of ESI and APCI ionization data facilitating high throughput and wi...

Page 30: ...rate downward to 100 nL min If a syringe pump is used a gas tight syringe is necessary to effect correct flow rates without leakage A volume of 250 L is recommended Borosilicate glass capillaries nano...

Page 31: ...UPLC System Guide for further details IntelliStart Fluidics system Functionality The IntelliStart Fluidics system is a solvent delivery system built into the mass spectrometer It delivers sample or s...

Page 32: ...ry out the operations processed by the software You can set IntelliStart fluidics configuration requirements in the system console You can edit the parameters frequency and extent of the automation Se...

Page 33: ...mass to charge ratios 4 The mass separated ions pass into the T Wave collision cell where they either undergo collision induced dissociation CID or pass to the second quadrupole Any fragment ions are...

Page 34: ...he selected ion recording SIR mode for quantitation when you cannot find a suitable fragment ion to perform a more specific multiple reaction monitoring MRM analysis see page 1 15 In SIR and MRM modes...

Page 35: ...ss Fragment precursor ions and pass all masses Scanning Precursor parent ion spectrum Scanning Static at product mass MRM Static at precursor mass Static at product mass PICS Product Ion Confirmation...

Page 36: ...de Typical applications Product ion mode is typically used for the following applications Method development for MRM screening studies Identifying product ions for use in MRM transitions Optimizing CI...

Page 37: ...R Because both MS1 and MS2 are static greater dwell time on the ions of interest is possible so the sensitivity achieved is better compared with scanning mode MS MS This mode is the most commonly used...

Page 38: ...background for all other components This enables fast characterization of potential matrix effects providing a platform for more robust method development Typical application You typically use RADAR m...

Page 39: ...Constant neutral loss mode Typical application You typically use constant neutral loss mode to screen mixtures for a specific class of compound that is characterized by a common fragmentation pathway...

Page 40: ...ructions for complete details Vacuum system An external roughing pump and an internal split flow turbomolecular pump combine to create the source vacuum The turbomolecular pump evacuates the analyzer...

Page 41: ...hows the rear panel locations of the connectors used to operate the instrument with external devices Instrument rear panel Power Source vent Source vacuum Nitrogen inlet Collision cell gas inlet Turbo...

Page 42: ...1 22 Specifications and Operating Modes...

Page 43: ...er describes how to start and shut down the instrument Contents Topic Page Starting the mass spectrometer 2 2 Preparing the IntelliStart Fluidics system 2 6 Rebooting the instrument 2 8 Leaving the ma...

Page 44: ...the rear panel ensure the nitrogen supply is connected to the instrument s nitrogen inlet connection see the figure on page 1 21 Requirement The nitrogen must be dry and oil free with a purity of at l...

Page 45: ...D the sample manager s run LED and the mass spectrometer s Operate LED remain off 6 Start MassLynx software and monitor the Instrument Console for messages and LED indications 7 Click IntelliStart in...

Page 46: ...nt indicate its operational status Power LED The power LED to the top right hand side of the mass spectrometer s front panel indicates when the instrument is powered on or powered off Operate LED The...

Page 47: ...ng to the following table If you are using an APCI interface IntelliStart software automatically sets the parameters according to the following table Flow rate versus temperature and gas flow Flow rat...

Page 48: ...arately to infuse smaller volumes The low volume vials have a volume of 1 5 mL Required material Chemical resistant powder free gloves To install the reservoir bottles Caution To avoid accidental spil...

Page 49: ...low volume vials 1 If a standard reservoir bottle is fitted remove it 2 Screw the low volume adaptor into the manifold and tighten it 3 Screw the low volume vial into the adaptor 4 For each low volum...

Page 50: ...ument s solvent delivery system can require more than one purge cycle to minimize carryover Rebooting the instrument The reset button causes the mass spectrometer to reboot Reboot the instrument when...

Page 51: ...maintenance When changing the source When leaving the mass spectrometer unused for a long period In these instances put the mass spectrometer in Standby mode See the online Help for details Emergency...

Page 52: ...2 10 Preparing for Operation...

Page 53: ...ray and atmospheric pressure chemical ionization APCI atmospheric pressure chemical ionization Combined Atmospheric Pressure Photoionization APPI APCI NanoFlow For details about other Waters and third...

Page 54: ...r working on the source see page 4 7 2 Remove the protective sleeve if fitted from the ESI probe tip Warning The LC system connections ESI probe and source can be contaminated with biohazardous and or...

Page 55: ...uster assembly 4 Tighten the probe locking ring to secure the probe in place Tip An automatic pressure test runs when the probe is correctly seated 5 Connect the ESI probe s cable to the high voltage...

Page 56: ...ve the ESI probe 1 Prepare the instrument for working on the source see page 4 7 2 Disconnect the tubing from the ESI probe 3 Disconnect the ESI probe cable from the high voltage connector 4 Unscrew t...

Page 57: ...i operation See the mass spectrometer s online Help for details on how to optimize the ESI probe for ESCi operation APCI mode APCI mode an option for the mass spectrometer produces singly charged prot...

Page 58: ...deprotonated in the negative ion mode The sample and reagent ions then pass through the sample cone and into the mass spectrometer Installing the IonSABRE II probe Required materials Chemical resistan...

Page 59: ...e probe adjuster assembly ensuring that the probe location dowel aligns with the probe adjuster assembly location hole 3 Tighten the probe locking ring to secure the probe in place Tip An automatic pr...

Page 60: ...stant powder free gloves To remove the IonSABRE II probe 1 Prepare the instrument for working on the source see page 4 7 2 Remove the corona pin see page 4 12 3 Disconnect the selector valve tubing fr...

Page 61: ...APPI lamp drive assembly is advanced into the source APPI mode The IonSABRE II probe introduces vaporized sample into the source where photons generated by an ultraviolet UV lamp mounted in the APPI...

Page 62: ...aporized sample into the source The sample passes between the sample cone and the corona pin which typically operates with a discharge current of 5 A The corona discharge generates ions that react wit...

Page 63: ...When the source is configured for dual operation in APCI mode current is applied to the corona pin but the repeller electrode is inactive Dual operation in APCI mode When the source is configured for...

Page 64: ...e enclosure The UV lamp which you ignite via a control in the MassLynx Tune window provides a constant photon output You vary the intensity of incident radiation upon the sample molecules by adjusting...

Page 65: ...nt for working on the source see page 4 7 Warning The source components can be contaminated with biohazardous and or toxic materials Always wear chemical resistant powder free gloves while performing...

Page 66: ...instrument s front panel connector 7 Connect the HT cable to the instrument s front panel connector 8 Install the IonSABRE II probe to the source and ensure that it is working correctly see page 3 6 T...

Page 67: ...e from the instrument s front panel 5 Remove the source enclosure see page 4 8 6 Remove the corona pin see page 4 15 7 Fit the blanking plug to the pin s mounting contact Warning The source components...

Page 68: ...three axis manipulator that slides on a pair of guide rails that allow its withdrawal from the source enclosure for maintenance and changes A light within the source provides illumination for the spr...

Page 69: ...ning The source components can be contaminated with biohazardous and or toxic materials Always wear chemical resistant powder free gloves while performing this procedure Warning To avoid electric shoc...

Page 70: ...of the NanoFlow source and your sprayer Tip For details regarding how to fit each sprayer see the corresponding reference Waters Universal NanoFlow Sprayer Installation and Maintenance Guide part num...

Page 71: ...ials Chemical resistant powder free gloves Needle nose pliers Borosilicate glass capillary Fused silica syringe needle or GELoader tip Fused silica cutter Caution The NanoFlow stage contains a high vo...

Page 72: ...m the end of the sprayer assembly 6 Remove the existing capillary from the sprayer Warning To avoid lacerations puncture injuries and possible contamination with biohazardous and toxic samples do not...

Page 73: ...ple Recommendation When using a GELoader tip break the glass capillary in half scoring it with a fused silica cutter so that the GELoader can reach the capillary s tip 9 Thread the knurled nut and app...

Page 74: ...ing obtained a signal you must adjust the tip position to maximize it Using the three axis manipulator you can adjust the tip position up and down left and right forward and backward As a starting poi...

Page 75: ...Should the spray stop you can restart it To do so in the Tune window set Capillary to 0 kV Then adjust the three axis manipulator so that viewed under magnification the capillary tip touches the sampl...

Page 76: ...3 24 Changing the Mode of Operation...

Page 77: ...efitting the source enclosure 4 8 Installing and removing the corona pin 4 12 Operating the source isolation valve 4 16 Removing O rings and seals 4 19 Cleaning the instrument case 4 20 Emptying the e...

Page 78: ...e source assembly seals 4 92 Replacing the air filter 4 95 Replacing the roughing pump oil 4 97 Replacing the roughing pump s oil demister element 4 100 APPI APCI source changing the UV lamp bulb 4 10...

Page 79: ...nt exhaust line Check daily empty as required See page 4 20 Gas ballast the roughing pump ESI weekly See page 4 22 Inspect and adjust the roughing pump oil level Weekly See page 4 25 Clean the source...

Page 80: ...ESCi modes When the corona pin is corroded or black or the sensitivity decreases to unacceptable levels See page 4 84 Replace the IonSABRE II probe heater Options using the IonSABRE II probe only If t...

Page 81: ...contaminate the roughing pump oil reduce this period which must be determined from experience See page 4 100 Clean the APPI APCI source UV lamp window When the window becomes visibly dirty or when the...

Page 82: ...e collaboration option that controls the two way connection with the ACQUITY UPLC system by enabling the Connections INSIGHT iAssist service level Consult these sources for more information about Conn...

Page 83: ...ts Warning To prevent injury always observe Good Laboratory Practice when handling solvents changing tubing or operating the instrument Know the physical and chemical properties of the solvents used s...

Page 84: ...erate indicator is not illuminated 3 Wait 3 minutes to allow the desolvation gas flow to cool the probe and source 4 In the Instrument Console click API to stop the desolvation gas flow 5 Lift the vis...

Page 85: ...nectors 4 Pull the source enclosure release located at the bottom right hand side outward and swing open the enclosure Warning The source components can be contaminated with biohazardous and or toxic...

Page 86: ...closure and lift it vertically off the two supporting studs on the source adaptor housing 6 Store the cables neatly by plugging them into the cable storage positions on the rear of the source enclosur...

Page 87: ...ster and options cables to the instrument s connectors Warning The source components can be contaminated with biohazardous and or toxic materials Always wear chemical resistant powder free gloves whil...

Page 88: ...ug from the corona pin mounting contact Tip Store the blanking plug in a safe location Warning The LC system connections ESI probe and source can be contaminated with biohazardous and or toxic materia...

Page 89: ...na pin mounting contact 4 Fit the corona pin to the mounting contact ensuring that the corona pin is securely mounted Warning The corona pin tip is sharp To avoid puncture wounds handle it with care T...

Page 90: ...5 Close the source enclosure 6 Look through the source window and use the vernier probe adjuster to position the probe tip so that it is pointing approximately midway between the tips of the sample c...

Page 91: ...lug to the corona pin mounting contact see the figure on page 4 13 5 Close the source enclosure Warning The LC system connections ESI probe and source can be contaminated with biohazardous and or toxi...

Page 92: ...swing open the enclosure Warning The source components can be contaminated with biohazardous and or toxic materials Always wear chemical resistant powder free gloves while performing this procedure Wa...

Page 93: ...Operating the source isolation valve 4 17 3 Close the source isolation valve by moving its handle counterclockwise to the vertical position Isolation valve handle in closed position...

Page 94: ...e source components can be contaminated with biohazardous and or toxic materials Always wear chemical resistant powder free gloves while performing this procedure Warning To avoid puncture wounds take...

Page 95: ...tool 1 to impale the O ring or seal 2 Pull the O ring or seal from its groove if necessary use tool 2 as an aid 3 Dispose of the O ring or seal in accordance with local environmental regulations Caut...

Page 96: ...nt Emptying the exhaust trap bottle Inspect the exhaust trap bottle in the instrument s exhaust line daily and empty it before it is more than 10 full Nitrogen exhaust trap bottle Caution Do not use a...

Page 97: ...xhaust trap bottle to the cap 6 Secure the nitrogen exhaust trap bottle in the upright position 7 Close the source enclosure Tip A leak test runs automatically If the test results in a failure ensure...

Page 98: ...Gas ballast the roughing pump when these conditions apply With ESI operation once a week When the roughing pump oil appears cloudy When the vacuum pressure is higher than normal When condensate forms...

Page 99: ...g pump 1 Use the flat blade screwdriver to turn the gas ballast valve on the pump a quarter turn to the open position Warning To avoid burn injuries take great care while working with the roughing pum...

Page 100: ...a handle operated gas ballast valve To gas ballast the roughing pump 1 Move the gas ballast valve handle on the pump counterclockwise from the horizontal position to the vertical position Warning To...

Page 101: ...l level appears in the roughing pump s oil level sight glass Check the oil level weekly you must maintain the oil level at or near the indicated maximum level when the pump is not operating Tip The oi...

Page 102: ...y the O ring groove depth in the plug Over tightening does not improve the plug seal it only makes the plug difficult to remove later Tip After you add oil to the pump the following situations can occ...

Page 103: ...components Clean the sample cone and cone gas nozzle when these conditions apply The sample cone and cone gas nozzle are visibly fouled You dismissed LC and sample related causes for decreased signal...

Page 104: ...e source components can be contaminated with biohazardous and or toxic materials Always wear chemical resistant powder free gloves while performing this procedure Warning To avoid electric shock ensur...

Page 105: ...egrees moving the handle from the vertical to the horizontal position 3 Slide the sampling cone assembly out of the ion block assembly Caution Do not open the isolation valve if the sampling cone asse...

Page 106: ...ction tool To disassemble the sampling cone assembly 1 Slide the collar to the end of the combined 2 5 mm Allen wrench and cone extraction tool 2 Insert the collar in the sample cone Warning The sampl...

Page 107: ...ounter clockwise and then lift them to remove the sample cone from the cone gas nozzle 4 Remove the O ring from the sample cone Caution The sample cone is fragile Never place it on its tip always plac...

Page 108: ...Appropriately sized glass vessels in which to completely immerse components when cleaning Use only glassware not previously cleaned with surfactants HPLC grade or better methanol HPLC grade or better...

Page 109: ...sonic bath for 20 minutes b Remove any residual water from the extraction cone by immersing it in a glass vessel containing methanol and then place the vessel in the ultrasonic bath for 10 minutes 5 C...

Page 110: ...tain a new one before reassembling the sampling cone assembly Assembling the sampling cone assembly Required material Clean chemical resistant powder free gloves To assemble the sampling cone assembly...

Page 111: ...hat the source isolation valve is in the closed position see page 4 16 Warning The source components can be contaminated with biohazardous and or toxic materials Always wear chemical resistant powder...

Page 112: ...e the sampling cone assembly into the ion block assembly 3 Grasp the cone gas nozzle handle and use it to rotate the sampling cone assembly 90 degrees moving the handle downward from the horizontal to...

Page 113: ...e mass spectrometer s online Help for details 2 Close the source isolation valve see page 4 16 Warning The source components can be contaminated with biohazardous and or toxic materials Always wear ch...

Page 114: ...llen wrench and cone extraction tool to unscrew the 4 captive screws that secure the ion block assembly 4 Remove the ion block assembly from the PEEK ion block support TP03130 Screws securing the Ion...

Page 115: ...ined 2 5 mm Allen wrench and cone extraction tool to loosen the captive screws securing the 2 PEEK extraction cone retainer clips and then rotate the retainer clips clear of the extraction cone Warnin...

Page 116: ...y sized glass vessel in which to completely immerse the extraction cone when cleaning Use only glassware not previously cleaned with surfactants HPLC grade or better methanol HPLC grade or better wate...

Page 117: ...ion do as follows a Rinse the extraction cone by immersing it in a glass vessel containing water and then placing the vessel in the ultrasonic bath for 20 minutes b Dry the components by immersing the...

Page 118: ...on cone to the ion block Required materials Chemical resistant powder free gloves Combined 2 5 mm Allen wrench and cone extraction tool To fit the extraction cone to the ion block 1 Fit the extraction...

Page 119: ...ion block assembly securing screws sequentially and in small increments 3 Open the source isolation valve see page 4 16 4 Close the source enclosure Warning The source components can be contaminated...

Page 120: ...mm Allen wrench and cone extraction tool O ring removal kit Needle nose pliers To disassemble the ion block assembly 1 Remove the ion block assembly from the source assembly see page 4 37 2 Ensure tha...

Page 121: ...llen wrench and cone extraction tool to loosen the 2 ion block cover plate captive securing screws 6 Remove the ion block cover plate Caution To ensure correct operation of the ion block assembly afte...

Page 122: ...ation valve O ring see page 4 19 9 If the isolation valve O ring shows signs of deterioration or damage dispose of it in accordance with local environmental regulations Warning The isolation valve O r...

Page 123: ...e ion block 12 Holding the PEEK ion block gently use the needle nose pliers to gently grasp the heat shrink tubing on the heater cartridge assembly and carefully slide it and the PEEK terminal block o...

Page 124: ...ve the cone gas O ring from the ion block 15 If the cover seal or cone gas O ring shows signs of deterioration or damage dispose of it in accordance with local environmental regulations Warning The co...

Page 125: ...lock blanking plug and associated seal 17 If the blanking plug seal shows signs of deterioration or damage dispose of it in accordance with local environmental regulations Warning The blanking plug se...

Page 126: ...cone retainer clips 19 Remove the extraction cone from the ion block Caution Take great care not to damage the extraction cone aperture when removing the extraction cone from the ion block The extrac...

Page 127: ...stant powder free gloves Appropriately sized glass vessels in which to completely immerse components when cleaning Use only glassware not previously cleaned with surfactants HPLC grade or better metha...

Page 128: ...lace the vessels in the ultrasonic bath for 10 minutes 4 Carefully remove the components from the vessels and blow dry them using inert oil free gas 5 Inspect each component for persisting contaminati...

Page 129: ...ndle 3 Fit the extraction cone to the ion block 4 Fit the 2 PEEK extraction cone retainer clips to the ion block 5 Use the combined 2 5 mm Allen wrench and cone extraction tool to tighten the captive...

Page 130: ...anol water applied to a lint free cloth to carefully clean the grooves 12 Fit the cover seal to the ion block ensuring that it is correctly seated Fit a new seal if the old one has been disposed of 13...

Page 131: ...the ion guide assembly if cleaning the ion block and isolation valve fails to increase signal sensitivity Removing the ion block assembly and ion guide from the source assembly Required materials Chem...

Page 132: ...ion block support 4 If any of the O rings show signs of deterioration or damage dispose of them in accordance with local environmental regulations Warning The O rings can be contaminated with biohaza...

Page 133: ...merse the ion guide when cleaning Use only glassware not previously cleaned with surfactants Length of small diameter PEEK or PTFE tubing appropriately sized for suspending the ion guide in the glass...

Page 134: ...a PEEK or PTFE tube into a hook shape 2 Insert one end of the hook into one of the holes in the ion guide s rear circuit board carrier Warning The ion guide PCB assemblies can be contaminated with bio...

Page 135: ...it using inert oil free gas 7 Inspect the ion guide assembly for persisting contamination If contamination is present do as follows a Use the wash bottle containing methanol to rinse the ion guide as...

Page 136: ...screws that secure the differential aperture to the 3 supporting rods 2 Remove the differential aperture from the ion guide Caution Sensitivity may be reduced if the differential aperture is aligned...

Page 137: ...en use 45 45 10 methanol water formic acid 4 Place the vessel in the ultrasonic bath for 30 minutes 5 If you used formic acid in the cleaning solution rinse the differential aperture by immersing it i...

Page 138: ...aperture is correctly aligned with the ion guide assembly look along the central axis of the ion guide from the ion entry side and confirm that the hole at the centre of the differential aperture is c...

Page 139: ...the ion block support to the source Required materials Chemical resistant powder free gloves 3 mm Allen wrench New seals and O rings Warning The source components can be contaminated with biohazardou...

Page 140: ...start fitting the O ring at the notch in the groove and then progressively work the ring into the groove in either direction from the notch 3 Fit the PEEK ion block support to the instrument s housin...

Page 141: ...t powder free gloves 7 mm wrench 10 mm wrench To remove the ESI probe tip and gasket 1 Remove the ESI probe from the source see page 3 4 Warning The probe and source components can be contaminated wit...

Page 142: ...Procedures 2 Use the 10 mm wrench to remove the probe tip Tip If the probe tip is difficult to remove use the 7 mm wrench in conjunction with the 10 mm wrench Probe tip 10 mm wrench Probe tip 7 mm wre...

Page 143: ...obe tip is damaged dispose of it in accordance with local environmental regulations Fitting the ESI probe tip and gasket Required materials Chemical resistant powder free gloves 10 mm wrench New metal...

Page 144: ...ulizer adjuster knob to adjust the capillary so that it protrudes by approximately 0 5 mm from the end of the probe 5 Fit the ESI probe to the source see page 3 2 Warning The probe and source componen...

Page 145: ...nch Needle nose pliers To remove the existing capillary 1 Remove the probe from the source see page 3 4 2 Retrieve the combined 2 5 mm Allen wrench and cone extraction tool from its storage location o...

Page 146: ...n wrench and cone extraction tool to remove the 3 screws retaining probe end cover 4 Remove the end cover and gasket from the probe assembly 5 Unscrew and remove the nebulizer adjuster knob End cover...

Page 147: ...mple capillary 4 71 6 Use the 10 mm wrench to remove the probe tip Tip If the probe tip is difficult to remove use the 7 mm wrench in conjunction with the 10 mm wrench Probe tip 10 mm wrench Probe tip...

Page 148: ...remove the knurled collar from the UNF coupling 10 Remove the knurled collar and conductive sleeve from the capillary 11 Use the 7 mm wrench to loosen the locknut Tip Use the 8 mm wrench to steady the...

Page 149: ...new capillary Required materials Chemical resistant powder free gloves Combined 2 5 mm Allen wrench and cone extraction tool 10 mm wrench Needle nose pliers LC pump HPLC grade or better 1 1 acetonitr...

Page 150: ...probe inlet connector and screw the connector finger tight into the PEEK union Rationale Doing so ensures a minimum dead volume when fitting the capillary 3 Fit the UNF coupling to the new capillary...

Page 151: ...10 Tighten the knurled collar to the UNF coupling 11 Perform a leak test by attaching the free end of the PEEK tubing to an LC pump and pumping 50 50 acetonitrile water through it at 1 mL min If leak...

Page 152: ...nd cone extraction tool to fit and tighten the 3 screws retaining the probe end cover 20 Return the combined 2 5 mm Allen wrench and cone extraction tool to its storage location on the source adaptor...

Page 153: ...s To clean the IonSABRE II probe tip 1 On the Instrument Console system tree click Xevo TQ S Manual optimization 2 On the Manual Optimization page click to stop the liquid flow 3 Click Gas to start th...

Page 154: ...2 5 mm Allen wrench and cone extraction tool To remove the existing capillary 1 Remove the probe from the source see page 3 8 2 Retrieve the combined 2 5 mm Allen wrench and cone extraction tool from...

Page 155: ...one extraction tool to remove the 3 screws retaining the probe end cover 4 Remove the end cover and gasket 5 Unscrew and remove the nebulizer adjuster knob 6 Remove the PEEK union UNF coupling assembl...

Page 156: ...EEK union from the UNF coupling 9 Remove the ferrule from the capillary 10 Remove the capillary from the UNF coupling 11 Dispose of the capillary and ferrule in accordance with local environmental reg...

Page 157: ...ed PEEK tubing approximately 60 cm 24 inches long Requirement Cut the tubing squarely that is perpendicular to its horizontal axis 2 Insert one end of the red PEEK tubing in the probe inlet connector...

Page 158: ...ee end of the PEEK tubing to an LC pump and pumping 50 50 acetonitrile water through it at 1 mL min If leakage occurs disassemble and remake the connection and repeat the leak test If the backpressure...

Page 159: ...e extraction tool to fit and tighten the 3 screws retaining the probe end cover 20 Return the combined 2 5 mm Allen wrench and cone extraction tool in its storage location on the source adaptor housin...

Page 160: ...h the lapping film and a methanol saturated tissue 3 Install the corona pin in the source see page 4 12 Warning The probe and source components can be contaminated with biohazardous and or toxic mater...

Page 161: ...IonSABRE II probe heater Required material Chemical resistant powder free gloves To remove the IonSABRE II probe heater 1 Remove the probe from the source see page 3 8 Warning The probe and source com...

Page 162: ...ew IonSABRE II probe heater Required materials Chemical resistant powder free gloves IonSABRE II probe heater Caution To avoid damaging the probe heater s electrical connections do not twist the heate...

Page 163: ...nt see page 3 6 5 In the Instrument Console click API to start the desolvation gas Replacing the ion block source heater Replace the ion block source heater if it fails to heat when the instrument is...

Page 164: ...lock source heater 1 Remove the ion block assembly from the instrument see page 4 37 2 Ensure that the isolation valve is closed Warning The ion block assembly can be contaminated with biohazardous an...

Page 165: ...ater 4 89 3 Use the combined 2 5 mm Allen wrench and cone extraction tool to loosen the 2 captive screws securing the ion block cover plate 4 Remove the ion block cover plate Ion block cover plate sec...

Page 166: ...cone extraction tool to remove the 2 screws securing the heater wires to the PEEK terminal block 6 Use the needle nose pliers to carefully swing the ring terminal tags out of the terminal block Heate...

Page 167: ...5 mm Allen wrench and cone extraction tool to fit and tighten the 2 screws securing the heater wires to the PEEK terminal block 12 Fit the ion block cover plate to the ion block assembly and then use...

Page 168: ...oves O ring removal kit To remove the probe adjuster assembly probe and source enclosure seals 1 Remove the source enclosure from the instrument see page 4 8 2 Use the O ring removal kit to carefully...

Page 169: ...emoval kit to carefully remove the following seals from the source enclosure Source enclosure seal Nebulizer gas seal Desolvation gas seal Probe adjuster nebulizer gas seal Probe adjuster assembly pro...

Page 170: ...lean the grooves 2 Fit the new source enclosure seal to the source enclosure Tip Start by feeding the seal into the groove at the bottom right hand corner and then working around the seal in a counter...

Page 171: ...to the probe adjuster assembly Probe seal Nebulizer gas seal 5 Refit the source enclosure to the instrument see page 4 11 Replacing the air filter You must replace the air filter annually Required ma...

Page 172: ...96 Maintenance Procedures 3 Disconnect the probe cable from the high voltage connector and leave the cable in a position that does not obstruct the air filter grill TP03403 Air filter grill Probe cabl...

Page 173: ...neath the metal lip 7 Close the air filter grill 8 Connect the probe cable to the high voltage connector 9 Close the source enclosure 10 Lower the instrument s visor Replacing the roughing pump oil Ch...

Page 174: ...the pump before draining 2 Vent and shut down the instrument see the mass spectrometer s online Help for details 3 Allow the roughing pump to cool 4 Place the container for used oil under the pump s d...

Page 175: ...n plug is clean and properly seated 10 Use the 8 mm Allen wrench to fit and tighten the roughing pump s oil drain plug Warning The roughing pump oil can be irritant or contaminated with biohazardous a...

Page 176: ...ing situations can occur The oil level drops slightly during the first month of operation The oil changes color darkens over time After the pump runs for 12 to 48 hours a few drops of oil often appear...

Page 177: ...ectrometer s online Help for details 2 Allow the roughing pump to cool 3 Use the 6 mm Allen wrench to remove the 4 bolts securing the exhaust flange to the roughing pump Warning The pump oil can be ir...

Page 178: ...ully remove the exhaust flange and oil demister element from the roughing pump 5 Use the 10 mm wrench to remove the nut that secures the oil demister element to the exhaust flange TP02693 Oil demister...

Page 179: ...rom the oil demister element 7 Remove the spring from the oil demister element 8 Dispose of the oil demister element in accordance with local environmental regulations Warning The oil demister element...

Page 180: ...ter element slightly elevated to prevent the loss of the spring fit the exhaust flange to the oil demister element Warning The pump oil can be irritant or contaminated with biohazardous or toxic analy...

Page 181: ...ge to the roughing pump 5 Use the 6 mm Allen wrench to fit the 4 bolts securing the exhaust flange to the roughing pump Caution The nut that secures the oil demister element to the exhaust flange must...

Page 182: ...tion tool from its storage location on the source adaptor housing 4 Hook the short end of the Allen wrench through the ring on the back of the bulb extraction plug and tug to remove it Warning The sou...

Page 183: ...ts storage location on the source adaptor housing 10 Close the source enclosure 11 Slide closed the instrument s source interface door APPI APCI source cleaning the lamp window The transmission of the...

Page 184: ...g Tip An automatic pressure test runs each time you close the source enclosure and when the instrument starts Removing the APPI lamp drive assembly seals Required materials Chemical resistant powder f...

Page 185: ...the lamp drive assembly and store it in a secure place see page 4 106 3 Retrieve the combined 2 5 mm Allen wrench and cone extraction tool from its storage location on the source adaptor housing 4 Us...

Page 186: ...ol to remove the remaining 2 lamp drive cover screws which were hidden by the release handle 8 Slide the cover off the lamp drive 9 Use the 3 mm Allen wrench to remove the 4 source enclosure cover scr...

Page 187: ...amp drive seals 4 111 11 Use the combined 2 5 mm Allen wrench and cone extraction tool to unscrew the 4 mounting flange screws Tip Take care not to drop the screws inside the lower cover TP03205 Mount...

Page 188: ...Using the small Phillips screwdriver remove the electrode screw and repeller electrode 14 Use the combined 2 5 mm Allen wrench and cone extraction tool to remove the two insulator screws 15 Remove the...

Page 189: ...emoval kit to carefully remove the O ring sealing the lamp bulb from inside the bulb holder see page 4 19 18 Use the O ring removal kit to carefully remove the two O rings from inside the lamp mountin...

Page 190: ...from the APPI source enclosure side 20 Dispose of the O rings in accordance with local environmental regulations Warning The O rings can be contaminated with biohazard ous and or toxic materials Ensu...

Page 191: ...eat the O ring in the small radius at the bottom of the groove Then use a suitable tool one with a circular cross section to roll the remainder of the O ring into the groove 2 Fit the lamp bulb sealin...

Page 192: ...ing shaft insertion tool from the mounting shaft 10 Fit the PEEK insulator to the end of the mounting shaft 11 Use the combined 2 5 mm Allen wrench and cone extraction tool to fit and tighten the two...

Page 193: ...sure release handle and secure it with the 2 screws Refit and reconnect the source enclosure to the machine refit the corona pin and probe Refer to page 3 13 for instructions Replacing the instrument...

Page 194: ...4 118 Maintenance Procedures...

Page 195: ...des also include the hazard symbols with accompanying text statements describing the hazards and telling you how to avoid them This appendix presents all the safety symbols and statements that apply t...

Page 196: ...electric shocks ultraviolet radiation exposures and others When the following symbols appear in a manual s narratives or procedures their accompanying text identifies the specific risk and explains ho...

Page 197: ...ction Extinguish all nearby flames Do not use tubing that is or has been stressed or kinked Do not expose nonmetallic tubing to incompatible compounds like tetrahydrofuran THF and nitric or sulfuric a...

Page 198: ...surfaces of the mass spectrometer when the instrument is in Operate mode To avoid nonlethal electric shock make sure the instrument is in Standby mode before touching areas marked with this high volt...

Page 199: ...When operating this device follow standard quality control procedures and the equipment guidelines in this section Warning Waters instruments can be used to analyze or process potentially hazardous s...

Page 200: ...er Modifikationen an dem Ger t ohne die ausdr ckliche Genehmigung der f r die ordnungsgem e Funktionst chtigkeit verantwortlichen Personen kann zum Entzug der Bedienungsbefugnis des Systems f hren Avv...

Page 201: ...e trouvant proximit de l instrument Evitez d utiliser des tubes s v rement d form s ou endommag s Evitez d utiliser des tubes non m talliques avec du t trahydrofurane THF ou de l acide sulfurique ou n...

Page 202: ...iamenti nei tubi non metallici riducendo notevolmente la pressione di rottura dei tubi stessi Advertencia se recomienda precauci n cuando se trabaje con tubos de pol mero sometidos a presi n El usuari...

Page 203: ...Warnings that apply to all Waters instruments A 9 Nonmetallic Tetrahydrofuran THF Methylene chloride Dimethyl sulfoxide THF...

Page 204: ...tueuses Vorsicht Der Benutzer wird darauf aufmerksam gemacht dass bei unsachgem er Verwenddung des Ger tes die eingebauten Sicherheitseinrichtungen unter Umst nden nicht ordnungsgem funktionieren Atte...

Page 205: ...l instrument Vorsicht Zum Schutz gegen Feuer die Sicherungen nur mit Sicherungen ersetzen deren Typ und Nennwert auf den Tafeln neben den Sicherungsabdeckungen des Ger ts gedruckt sind Attenzione per...

Page 206: ...al symbols can appear in instrument user manuals and on the instrument s front or rear panels Electrical power on Electrical power off Standby Direct current Alternating current Protective conductor t...

Page 207: ...ng symbols A 13 Handling symbols These handling symbols and their associated text can appear on labels affixed to the outer packaging of Waters instrument and component shipments Keep upright Keep dry...

Page 208: ...A 14 Safety Advisories...

Page 209: ...echnical Service for recommended cleaning flushing and packaging procedures See page 4 7 Contents Topic Page External wiring and vacuum connections B 2 Connecting the oil filled roughing pump B 3 Conn...

Page 210: ...ear in the figure below Note Connectors and controls not identified in the following figure are for use by Waters engineers only Rear panel Power Source vent Source vacuum Nitrogen inlet Collision cel...

Page 211: ...W25 center rings included in the installation kit NW25 clamps included in the installation kit 10 mm reducer fitting included in the installation kit 10 mm ID nylon tube included in the installation k...

Page 212: ...when performing this procedure Caution To ensure correct operation of the roughing pump the pump must be installed within 1 degree of horizontal The area where the roughing pump is located must have...

Page 213: ...sing 2 hose clamps connect the opposite end of the length of 1 inch vacuum hose in step 4 to the 1 inch OD straight vacuum port on the instrument s rear panel 6 Connect the opposite end of the 10 mm n...

Page 214: ...age B 7 Caution The instrument requires two separate exhaust systems one for nitrogen the other for the roughing pump Vent them to atmosphere through separate exhaust lines Oil mist can severely damag...

Page 215: ...ctions To make the electrical connections to the roughing pump 1 Connect the relay cable from the roughing pump d c connector to the pump connector on the instrument s rear panel 2 Connect the roughin...

Page 216: ...uded in the installation kit NW25 center rings included in the installation kit NW25 clamps included in the installation kit NW40 center rings included in the installation kit Warning To avoid electri...

Page 217: ...ng pump Warning The pump and its connections can be contaminated with biohazardous and or toxic materials Always wear chemical resistant powder free gloves when performing this procedure Caution To en...

Page 218: ...end of the length of 1 inch vacuum hose in step 4 to the 1 inch OD straight vacuum port on the instrument s rear panel 6 Connect the opposite end of the 10 mm nylon tube to the 10 mm source vent port...

Page 219: ...the electrical connections to the oil free roughing pump 1 Connect the relay cable from the roughing pump d c connector to the pump connector on the mass spectrometer s rear panel 2 Connect the rough...

Page 220: ...o cut a 3 8 cm to 5 0 cm 1 5 inch to 2 inch length of 6 mm PTFE tubing 2 Connect this piece of tubing to one end of the nitrogen supply in line filter 3 Connect the remaining length of the 6 mm PTFE t...

Page 221: ...ions 5 Attach the nitrogen regulator to the nitrogen supply 6 Install the 6 mm stud into the regulator outlet 7 Connect the free end of the long piece of 6 mm PTFE tubing to the 6 mm stud Source vent...

Page 222: ...lok nut and ferrule to connect the 1 8 inch stainless steel tube to the collision cell gas inlet on the rear of the instrument see the figure on page B 13 2 Use the wrench to tighten the 1 8 inch Swag...

Page 223: ...0 20 kPa 2 mbar 0 03 psi below atmospheric pressure negative pressure The exhaust connections can be contaminated with biohazardous and or toxic materials Always wear chemical resistant powder free gl...

Page 224: ...ctions Exhaust trap bottle Warning Leaks in the source exhaust system can result in the release of biohazardous or toxic materials To confirm the integrity of the source exhaust system perform the fol...

Page 225: ...the barbed fitting of the drain located at the bottom of the instrument Warning The waste line and connection can be contaminated with biologically hazardous or toxic materials Always wear chemical re...

Page 226: ...of the waste solvent Positioning of drain tube Warning To prevent leakage of biologically hazardous or toxic materials ensure that the drain line does not crimp or bend A crimp or bend can impede flo...

Page 227: ...ed Instrument LAN on the workstation rear panel To connect the instrument to the power source 1 Select the correct power cord for your location 2 Connect the female end of the power cord to the power...

Page 228: ...cal connections to the rear panel from hazardous voltages by double or reinforced insulation Circuits of this type are classified as safety extra low voltage SELV Examples of circuits that are typical...

Page 229: ...Analog Out Analog Out Ground Not Used Not Used Stop Flow Out Stop Flow Out Ground Switch 2 Out Switch 2 Out Connector I 1 2 3 4 5 6 7 8 9 10 Inject Start In Inject Start In Ground Event In Event In Sw...

Page 230: ...ion Analog Out Used for analog chart output functionality The output voltage range is 0 to 1 V The resolution of the voltage output is 12 bits Stop Flow Out Used to stop the solvent flow if the nitrog...

Page 231: ...he signal cable to the connector 3 Slide the clamp with the bend facing down into the protective shield 4 Insert the clamp and shield with the bend facing down into the connection cover and loosely ti...

Page 232: ...nto the connection cover and position the clamp over the cable leads Tighten the clamp into place with the second self tapping screw 6 Place the second connection cover over the first cover and snap i...

Page 233: ...tacle on the rear panel of the instrument 2 Connect the male end of the instrument power cord to the 200 to 240 V AC wall outlet prepared as described in the instrument Site Preparation Guide 3 Connec...

Page 234: ...B 26 External Connections...

Page 235: ...arning To confirm the integrity of the source exhaust system you must address any safety issues raised by the contents of this Appendix Contents Topic Page Preventing contamination C 2 Items exposed t...

Page 236: ...on ingredients used to prepare mobile phases Items exposed to solvent Item Material Autotune reservoirs High density polyethylene Corona discharge pin mounting contact PEEK Gas exhaust port Aluminum G...

Page 237: ...Methanol Acetonitrile Formic acid 0 1 Acetic acid 0 1 Trifluoroacetic acid 0 1 Ammonium acetate 10 mM Ammonium formate 10 mM These solvents are not expected to cause any problems with the materials i...

Page 238: ...C 4 Materials of Construction and Compliant Solvents...

Page 239: ...connect the selector valve to the source probes use the PEEK tubing and natural beige colored PEEK finger tight screws specified on page D 4 Warning The IntelliStart Fluidics components can be contam...

Page 240: ...sor The letters etched on the front of the valve represent the components to which the associated port is connected Etched letters with a light background indicate inputs while letters with a dark etc...

Page 241: ...n specifications Caution When plumbing the IntelliStart Fluidics system use only the tubes nuts and ferrules outlined below to ensure over pressure protection The use of parts not recommended here can...

Page 242: ...ple reservoirs ports A and B is not user serviceable To replace the tubing contact Waters to arrange an engineer visit Replacement tubing specifications Port ID inches Color Length m R A and B 0 020 O...

Page 243: ...in 3 14 APPI APCI corona pin 3 11 APPI APCI source components 3 12 ASAP probe 1 10 assembling sampling cone 4 34 B biohazard warning A 4 burst warning A 3 C cables network B 19 source APPI APCI 3 14 E...

Page 244: ...ts ii D DART source 1 2 demister element replacing 4 5 4 100 DESI source 1 2 detector compliant solvents C 1 external wiring B 2 flow rates 2 5 I O signal connectors B 20 materials of construction C 1...

Page 245: ...ion guide assembly 4 63 waste liquid 4 21 handling symbols A 13 hazards flammable solvents iv high temperature vi removing mass spectrometer from service vi solvent leakage iv high temperature hazard...

Page 246: ...trometer calibrating 2 4 compatibility 1 2 leaving ready for operation 2 9 removing from service vi shock hazard A 4 specifications 1 2 starting up 2 2 tuning 2 4 MassLynx software 1 7 materials of co...

Page 247: ...B 2 rebooting detector 2 8 removing combined APPI APCI source 3 15 corona pin 4 15 ESI probe 3 4 ion block assembly 4 37 IonSABRE II probe 3 8 mass spectrometer from service vi O rings 4 19 sampling...

Page 248: ...ning 4 18 operating 4 16 preparing for work on 4 7 source components cleaning 4 27 sources APCI 1 9 APGC 1 2 APPI APCI 1 2 combined APPI APCI 1 9 DART 1 2 DESI 1 2 ESI 1 9 LDTD 1 2 NanoFlow ESI 1 2 1...

Page 249: ...ions B 2 system 1 20 valve source isolation 4 16 W warning symbols A 2 A 5 waste line connecting B 17 liquid handling 4 21 wiring external B 2 workstation connecting B 19 X Xevo TQD removing from serv...

Page 250: ...Index 8...

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