Waters Quattro Premier XE Operator'S Manual Download Page 29

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3-1

Operating in APPI, APCI, and
Dual APPI/APCI Modes

This chapter describes operation of the Quattro Premier XE Combined

APPI and APCI Source.

Contents:

Topic

Page

Operating in APPI mode

3-2

Operating in APCI mode

3-10

Operating in Dual APPI/APCI mode

3-13

Summary of Contents for Quattro Premier XE

Page 2: ...sumes no responsibility for any errors that may appear in this document This document is believed to be complete and accurate at the time of publication In no event shall Waters Corporation be liable...

Page 3: ...encounter in this document or to suggest ideas for otherwise improving it Please help us better understand what you expect from our documentation so that we can continuously improve its accuracy and u...

Page 4: ...consult your organization s safety representative for guidance When you develop methods follow the Protocol for the Adoption of Analytical Methods in the Clinical Chemistry Laboratory American Journal...

Page 5: ...boratory atmosphere of 10 LC eluate Warning To confirm the integrity of the source exhaust system renew the source O rings at intervals not exceeding one year To avoid chemical degradation of the sour...

Page 6: ...mperature hazard Mass spectrometer high temperature hazard Warning To avoid burn injuries avoid touching the source enclosure with your hand when operating or servicing the instrument Source enclosure...

Page 7: ...shock do not remove the mass spectrometer s protective panels The components they cover are not user serviceable To avoid nonlethal electric shock when the instrument is in Operate mode avoid touching...

Page 8: ...ersonnel should use the instrument for those purposes Calibrating To calibrate LC systems follow acceptable calibration methods using at least five standards to generate a standard curve The concentra...

Page 9: ...g samples from a complex matrix such as soil tissue serum plasma whole blood etc note that the matrix components can adversely affect LC MS results enhancing or suppressing ionization To minimize thes...

Page 10: ...the United Kingdom with the Medicines and Healthcare Products Regulatory Agency MHRA at Market Towers 1 Nine Elms Lane London SW8 5NQ The reference number is IVD000167 Waters Corporation Micromass UK...

Page 11: ...rating viii Quality control ix IVD authorized representative information x IVD authorized representative x 1 APPI APCI and Dual APPI APCI Modes 1 1 APPI mode 1 2 APCI mode 1 3 Dual APPI APCI mode 1 4...

Page 12: ...oving O rings and seals 4 3 Changing the UV lamp bulb 4 4 Cleaning the lamp window 4 7 Replacing the APPI lamp drive assembly O rings 4 9 Removing the APPI lamp drive assembly O rings 4 9 Fitting the...

Page 13: ...Table of Contents 3 B Materials of Construction and Compliant Solvents B 1 Preventing contamination B 2 Items exposed to solvent B 2 Solvents used to prepare mobile phases B 3 Index Index 1...

Page 14: ...4 Table of Contents...

Page 15: ...1 1 APPI APCI and Dual APPI APCI Modes This chapter describes the Quattro Premier XE Combined APPI and APCI Source operation modes Contents Topic Page APPI mode 1 2 APCI mode 1 3 Dual APPI APCI mode 1...

Page 16: ...d sample into the source where photons generated by an ultra violet UV lamp mounted in the APPI lamp drive assembly produce sample ions Direct photoionization of a sample molecule occurs when the phot...

Page 17: ...into the source The sample passes between the sample cone and the corona pin which typically operates with a discharge current of 5 A The corona discharge generates ions that react with the mobile ph...

Page 18: ...source is configured for dual operation in APCI mode current is applied to the corona pin but the repeller electrode is inactive Dual operation in APCI mode When the source is configured for dual ope...

Page 19: ...scribes the Quattro Premier XE Combined APPI and APCI Source components and how to install and remove the source Contents Topic Page Combined APPI APCI Source 2 2 Installing the Combined APPI APCI Sou...

Page 20: ...y comprises A UV lamp A repeller electrode A lamp holder A mounting shaft with indexing scales An aluminum enclosure housing drive electronics An APPI source enclosure side plate See also Figure APPI...

Page 21: ...APPI lamp drive assembly to move the whole assembly on its mounting shaft The mounting shaft s indexing numbers aid in setting this distance See also To obtain an ion beam and tune the instrument for...

Page 22: ...y showing the UV lamp and repeller electrode APPI lamp drive assembly fitted to the source enclosure APPI source enclosure side plate UV lamp Repeller electrode APPI lamp drive assembly Source enclosu...

Page 23: ...been installed the APPI options will not be available in the MassLynx Tune window Ion Mode menu Installing the APPI lamp drive assembly and APCI probe Note To complete this procedure you must perform...

Page 24: ...ring is in place on the right side of the source enclosure Warning To avoid self contamination with toxic and biohazardous materials always wear chemical resistant powder free gloves at all times whil...

Page 25: ...cable part number M955286BC1 between the APPI lamp drive assembly s HV electrical connection and the HV connector on the instrument front panel 17 Install the APCI probe to the source and ensure that...

Page 26: ...at the instrument status indicator is red 2 Wait for three minutes for the desolvation gas to cool the probe 3 Click to stop the nitrogen flow 4 Remove the APCI probe from the source See also The Wate...

Page 27: ...re the APPI lamp drive assembly to the source enclosure 13 Remove the APPI lamp drive assembly from the source enclosure 14 Ensure that the source enclosure side flange O ring is in place on the right...

Page 28: ...nstrument s front access door 20 Perform a pressure test on the source Warning To confirm the integrity of the source exhaust system you must perform a pressure test on the source as described in the...

Page 29: ...and Dual APPI APCI Modes This chapter describes operation of the Quattro Premier XE Combined APPI and APCI Source Contents Topic Page Operating in APPI mode 3 2 Operating in APCI mode 3 10 Operating...

Page 30: ...all it as described in Installing the Combined APPI APCI Source on page 2 5 2 Fully retract the lamp drive assembly 3 If the instrument has been in use a On the MassLynx Tune window click Press for St...

Page 31: ...sten the securing clips 8 Close the instrument s front access door Warning To avoid self contamination with toxic and biohazardous materials always wear chemical resistant powder free gloves while han...

Page 32: ...x Tune window APPI Source tab 2 Click to start the nitrogen flow 3 Click Press for Operate and confirm that the instrument status indicator is green 4 Click Switch Lamp On and confirm that the Lamp re...

Page 33: ...t up the HPLC pump with LC mobile phase methanol at 200 L min Warning To avoid self contamination with toxic and biohazardous materials always wear chemical resistant powder free gloves while handling...

Page 34: ...le This position gives the best compromise when first obtaining an ion beam Advancing the UV lamp further will increase the beam intensity but is more likely to contaminate the lamp window 4 Position...

Page 35: ...ng table Recommended Tune window APPI Source tab parameters Parameter Recommended value Voltages Repeller kV 1 50 Cone V 55 Extractor V 2 RF Lens V 0 0 Temperatures Source Temp C 120 APcI APPI Probe T...

Page 36: ...m that they have stabilized and are correct 10 Click Press for Operate and confirm that the instrument status indicator is green 11 On the Tune window APPI Source Tab click Switch Lamp On and confirm...

Page 37: ...Click to start the syringe pump 16 On the Tune window click the Function 1 box 17 In the adjacent drop down list click MS Scan 18 Enter Mass 128 0 Span 10 and Gain 150 Tip These values are recommende...

Page 38: ...s not already fitted on the instrument install it as described in Installing the Combined APPI APCI Source on page 2 5 2 Fully retract the lamp drive assembly 3 If the instrument has been in use a On...

Page 39: ...a pin in a safe location Warning To avoid self contamination with toxic and biohazardous materials always wear chemical resistant powder free gloves while handling the components The probe and source...

Page 40: ...the securing clips 9 Close the instrument s front access door To obtain an ion beam and tune the instrument for APCI mode Obtain an ion beam and tune the instrument as described in the Waters Quattro...

Page 41: ...l it as described in Installing the Combined APPI APCI Source on page 2 5 2 Fully retract the lamp drive assembly 3 If the instrument has been in use a On the MassLynx Tune window click Press for Stan...

Page 42: ...use the needle nose pliers to remove the pin Tip Store the APCI corona pin in a safe location Warning To avoid self contamination with toxic and biohazardous materials always wear chemical resistant p...

Page 43: ...orona pin alignment Note For clarity the lamp drive assembly is not shown 9 Close the source enclosure door and fasten the securing clips 10 Close the instrument s front access door To confirm that th...

Page 44: ...acent instrument status indicator is green 5 Click Switch Lamp On and confirm that the Lamp readback is green 6 Click Switch Lamp Off and confirm that the Lamp readback is red 7 Click Press for Standb...

Page 45: ...he instrument for APPI mode See also To obtain an ion beam and tune the instrument for APPI mode on page 3 6 Tip The Tune window APPI APcI Source tab APCI Voltages frame is not used at this stage 5 On...

Page 46: ...I APCI mode 8 Obtain an ion beam and tune the instrument for APCI mode See also The procedure in the Waters Quattro Premier XE Operator s Guide Caution To avoid damage to the UV lamp when operating in...

Page 47: ...APCI mode 3 19 To create an MS Method file 1 In the MassLynx window click the MS Method icon MS Method Editor 2 In the MS Method Editor click to delete the current entry from the MS Method Editor 3 Cl...

Page 48: ...n Editor closes and the parameters are now included as the APPI entry in the MS Method Editor 7 Repeat step 3 through step 6 in this case clicking the required APCI ionization mode API or API in the I...

Page 49: ...for the Quattro Premier XE Combined APPI and APCI Source Contents Topic Page General 4 2 Maintenance schedule 4 2 Safety and handling 4 3 Removing O rings and seals 4 3 Changing the UV lamp bulb 4 4 C...

Page 50: ...e lists periodic maintenance schedules that ensure optimum instrument performance The maintenance frequencies shown apply to instruments that normally receive moderate use Maintenance schedule Procedu...

Page 51: ...on the instrument O ring removal kit Warning To avoid self contamination with toxic and biohazardous materials always wear chemical resistant powder free gloves at all times while handling the compone...

Page 52: ...owder free gloves Small flat blade screwdriver Small cross head screwdriver Short length of length 4 mm nylon tube Warning To avoid self contamination with toxic and biohazardous materials always wear...

Page 53: ...the repeller electrode from the PEEK insulator Warning To avoid self contamination with toxic and biohazardous materials always wear chemical resistant powder free gloves at all times while handling...

Page 54: ...o the lamp drive assembly 8 Refit the cover cap to the lamp drive assembly 9 Fit the repeller electrode to the PEEK insulator 10 Use the cross head screwdriver to fit and tighten the repeller electrod...

Page 55: ...eep the surface clear of contamination and avoid reduced sensitivity Required materials Chemical resistant powder free gloves Lint free cloth Methanol or isopropyl alcohol To clean the lamp window 1 O...

Page 56: ...ion with toxic and biohazardous materials always wear chemical resistant powder free gloves at all times while handling the components The probe and source may be contaminated with biologically hazard...

Page 57: ...gloves 2 5 mm Allen wrench O ring removal kit To remove the APPI lamp drive assembly O rings 1 Remove the lamp bulb See also To change the UV lamp bulb on page 4 5 step 1 through step 6 Warning To ens...

Page 58: ...e lamp bulb sealing O ring from inside the end of the mounting shaft See also Removing O rings and seals on page 4 3 5 Extract the mounting shaft from the APPI source enclosure side plate 6 Use the O...

Page 59: ...ove the lamp mounting flange from the APPI source enclosure side plate 9 Use the O ring removal kit to carefully remove the lamp mounting flange O ring from the APPI source enclosure side plate See al...

Page 60: ...rs Tip If contamination is present use 1 1 methanol water applied to the lint free cloth to carefully clean the grooves 2 Fit the new lamp mounting flange O ring to the APPI source enclosure side plat...

Page 61: ...the source enclosure side plate then use a suitable tool having a circular cross section to roll the remainder of the O ring into the groove 6 Fit the mounting shaft insertion tool to the mounting sha...

Page 62: ...y 17 Fit the APPI lamp drive assembly onto the right side of the source enclosure 18 Fit and tighten the three securing thumbscrews See also Figure APPI lamp drive assembly and side plate on page 2 3...

Page 63: ...V electrical connection and the HV connector on the instrument front panel 22 Close the instrument s front access door 23 Perform a pressure test on the source Warning To confirm the integrity of the...

Page 64: ...4 16 Maintenance Procedures...

Page 65: ...des also include the hazard symbols with accompanying text statements describing the hazards and telling you how to avoid them This appendix presents all the safety symbols and statements that apply t...

Page 66: ...ks include burn injuries electric shocks ultraviolet radiation exposures and others When the following symbols appear in a manual s narratives or procedures their accompanying text identifies the spec...

Page 67: ...g around such tubing Wear eye protection Extinguish all nearby flames Do not use tubing that is or has been stressed or kinked Do not expose nonmetallic tubing to incompatible compounds like tetrahydr...

Page 68: ...l surfaces of the mass spectrometer when the instrument is in Operate mode To avoid non lethal electric shock make sure the instrument is in Standby mode before touching areas marked with this high vo...

Page 69: ...When operating this device follow standard quality control procedures and the equipment guidelines in this section Warning Waters instruments can be used to analyze or process potentially hazardous s...

Page 70: ...n oder Modifikationen an dem Ger t ohne die ausdr ckliche Genehmigung der f r die ordnungsgem e Funktionst chtigkeit verantwortlichen Personen kann zum Entzug der Bedienungsbefugnis des Systems f hren...

Page 71: ...e trouvant proximit de l instrument Evitez d utiliser des tubes s v rement d form s ou endommag s Evitez d utiliser des tubes non m talliques avec du t trahydrofurane THF ou de l acide sulfurique ou n...

Page 72: ...no rigonfiamento nei tubi non metallici riducendo notevolmente la resistenza alla rottura dei tubi stessi Advertencia se recomienda precauci n cuando se trabaje con tubos de pol mero sometidos a presi...

Page 73: ...Warnings that apply to all Waters instruments A 9...

Page 74: ...isque d tre d fectueuses Vorsicht Der Benutzer wird darauf aufmerksam gemacht dass bei unsachgem er Verwenddung des Ger tes unter Umst nden nicht ordnungsgem funktionieren Attenzione l utente deve ess...

Page 75: ...de la m me puissance afin d viter tout risque d incendie Vorsicht Zum Schutz gegen Feuergefahr die Sicherungen nur mit Sicherungen des gleichen Typs und Nennwertes ersetzen Attenzione per una buona p...

Page 76: ...prise avant d effectuer la maintenance de l instrument Vorsicht Zur Vermeidung von Stromschl gen sollte das Ger t vor der Wartung vom Netz getrennt werden Attenzione per evitare il rischio di scossa e...

Page 77: ...ls These can appear in instrument user manuals and on the instrument s front or rear panels Electrical power on Electrical power off Standby Direct current Alternating current Protective conductor ter...

Page 78: ...visories Handling symbols These handling symbols and their associated text can appear on labels affixed to the outer packaging of Waters instrument and component shipments Keep upright Keep dry Fragil...

Page 79: ...ts Warning To confirm the integrity of the source exhaust system you must address any safety issues raised by the contents of this Appendix Topic Page Preventing contamination B 2 Items exposed to sol...

Page 80: ...the solvents normally used with these items See Solvents used to prepare mobile phases on page B 3 for details about the most common ingredients used to prepare mobile phases Items exposed to solvent...

Page 81: ...o cause any problems with the materials identified in Items exposed to solvent on page B 2 Probe adjuster assembly Anodized aluminium glass filled acetal and stainless steel Probe shaft PEEK Push in g...

Page 82: ...B 4 Materials of Construction and Compliant Solvents...

Page 83: ...3 C cables drive 2 7 4 14 HT 2 7 4 15 caution symbol A 5 chemical hazard warning A 5 cleaning the UV lamp window 4 7 combined APPI APCI source installing 2 5 removing 2 8 compliant solvents B 1 conne...

Page 84: ...O rings lamp bulb sealing 4 9 lamp mounting flange 4 9 mounting shaft 4 9 replacing the 4 9 O rings removing 4 3 P pin corona APCI 1 3 3 14 dual APPI APCI 1 4 3 11 3 15 pressure test performing 2 5 2...

Page 85: ...3 I warning A 2 syringe pump 3 5 syringe type selecting 3 9 T Tune window Analyser tab 3 8 APPI Source tab 3 4 U UV lamp 2 2 cleaning window 4 7 replacing bulb 4 5 turning on 2 3 W warning symbols A...

Page 86: ...Index 4...

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Waters Quattro Premier XE, Operator'S Manual, Page: 29 / 86

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