Waters 2489, Operator'S Manual

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xii Table of Contents
Operating the detector in dual wavelength mode ........................................ 3-32
Changing from single to dual wavelength mode ..................................... 3-33
Obtaining a RatioPlot ............................................................................... 3-34
Obtaining a MaxPlot ................................................................................. 3-35
Programming timed events, threshold events, and methods ...................... 3-35
Timed events ............................................................................................. 3-36
Threshold events ....................................................................................... 3-39
Storing a method ....................................................................................... 3-40
Retrieving a method .................................................................................. 3-41
Viewing events within a method .............................................................. 3-42
Resetting a method ................................................................................... 3-42
Clearing events ......................................................................................... 3-43
Scanning spectra ............................................................................................. 3-44
Before you begin............................................................................................. 3-44
Scanning new spectra .................................................................................... 3-48
Zero scan .................................................................................................... 3-49
Running the sample scan .......................................................................... 3-52
Storing a spectrum......................................................................................... 3-56
Getting information about a stored spectrum .............................................. 3-56
Reviewing a stored spectrum ........................................................................ 3-57
Subtracting a spectrum ................................................................................. 3-57
Replaying a spectrum .................................................................................... 3-58
Scanning using the cuvette ........................................................................... 3-59
Before you begin ........................................................................................ 3-60
Cuvette scanning procedure ..................................................................... 3-60
Scanning using a flow cell and a syringe...................................................... 3-62
Conserving lamp life ...................................................................................... 3-62
Shutting down the detector ........................................................................... 3-65
Removing buffered mobile phase ............................................................. 3-65
Shutting down the detector ...................................................................... 3-65