Waters 2489, Operator'S Manual

Page: 219 / 234

Wavelength selection D-13
*Willard, H. H. and others. Instrumental Methods of Analysis, 6th ed. Litton Educational Publishing,
Inc., 1981. Reprinted by permission of Wadsworth Publishing Co., Belmont, California, 94002.
Nitrate —ONO2 270
(shoulde
r) 12
Allene —(C=C)2—
(acyclic) 210-230 21,000
Allene —(C=C)3— 260 35,000
Allene —(C=C)4— 300 52,000
Allene —(C=C)5— 330 118,000
Allene —(C=C)2—
(alicyclic) 230-260 3000-8000
Ethylenic/
Acetylenic C=C—C
≡
C 219 6,500
Ethylenic/
Amido C=C—C=N 220 23,000
Ethylenic/
Carbonyl C=C—C=O 210-250 10,000-
20,000
Ethylenic/
Nitro C=C—NO2 229 9,500
Electronic absorption bands of representative chromophores*
Chromophore
Chemical
Configuration
λ
max
(nm)
∈
max
(L/m/cm)
λ
max
(nm)
∈
max
(L/m/cm)