3-62
Preparing the Detector
Rules:
•
To prevent invalid subsequent chromatographic results, remove the
cuvette from the detector and replace the empty holder after
running your cuvette scans.
•
To maintain optimum system performance, replace the front left
panel cover before resuming normal operation of the detector.
6.
Insert a reference cuvette containing the mobile phase standard and run
a zero scan.
7.
Replace the reference cuvette with a cuvette containing your analyte
dissolved in mobile phase solvent and run a sample scan.
8.
Use the storage, review, subtract and review, and replay functions to
analyze the data obtained.
Scanning using a flow cell and a syringe
If you do not have a cuvette, you can scan using a manually filled flow cell.
Requirement:
Before you scan using a flow cell, make sure there is no cuvette
in the cuvette holder.
To run a spectrum using a flow cell:
1.
With a syringe, fill the flow cell with the mobile phase or solvent in
which your sample is dissolved.
2.
Run a zero scan following the procedure
3.
With a syringe, fill the flow cell with the analyte, then run a sample scan
following the procedure in
Use the storage, review, subtract and review, and replay functions of the
detector to compare the scanned data.
Conserving lamp life
You can conserve the lamp without powering-off the detector by leaving the
instrument on and turning off only the deuterium lamp.
Tip:
If the detector is operating under remote control, you can program the
controller to turn the lamp off and on without using the detector front panel.
Summary of Contents for 2489
Page 8: ...viii...
Page 32: ...1 16 Theory and Principles of Operation...
Page 134: ...3 66 Preparing the Detector...
Page 193: ...Warnings that apply to all Waters instruments A 9...
Page 204: ...B 6 Detector Specifications...
Page 220: ...D 14 Solvent Considerations...
