ThermoFisher Scientific Vanquish VC-D11, Operating Manual, Page: 163 / 192

8 • Troubleshooting
Diode Array/Multiple-Wavelength Detectors (VC-D11, VC-D12, VF-D11)
Operating Manual Page 163
Message and Code Description and Remedial Action
Code 3132 Holmium validation failed –
wavelength outside limits. • Repeat the validation.
• Make sure that the lamp(s) is/are turned on, perform a
wavelength calibration and repeat the wavelength validation
again.
• Ensure that the flow cell is installed properly. Remove and install
the flow cell. Flush the flow cell with pure LC/MS-grade water
with a flow rate of > 1 mL/min for several minutes.
• Check the available lamps: If the available lamps were newly
installed, ensure that they are installed properly. If they are old
(counting, for example, more than 2000 operating hours),
replace the available lamps (see
Lamps (
}
page 124) ).
• Install a different flow cell or, if available, a diagnostic cell.
Perform a validation. Perform a calibration and repeat the
validation again. If the message disappears with a different cell,
the flow cell that was previously installed may be contaminated.
Perform a flushing procedure with this cell (see
Preparing the
Flow Cell for Storage ( } page 132) ).
• If the problem persists, contact Technical Support.
Code 3133 Holmium validation failed –
specified wavelength not found. • Repeat the validation.
• Make sure that the available lamps are on, perform a calibration
and repeat the validation again.
• Ensure that the flow cell is installed properly. Remove and install
the flow cell. Flush the flow cell with pure LC/MS-grade water
with a flow rate of > 1 mL/min for several minutes.
• Check the available lamps: If a lamp is newly installed, ensure
that it is installed properly. If a lamp is old (counting, for
example, more than 2000 operating hours), replace it (see
Lamps (
}
page 124) ).
• Install a different flow cell or, if available, a diagnostic cell.
Perform a validation. Perform a calibration and repeat the
validation again. If the message disappears with a different cell,
the flow cell that was previously installed may be contaminated.
Perform a flushing procedure with this cell (see
Preparing the
Flow Cell for Storage ( } page 132) ).
• If the problem persists, contact Technical Support.
Code 3153 Bad calibration – check flow
cell. An error occurred during wavelength calibration.
Check the flow cell (see Checking the Flow Cell ( } page 166) ).
If the message still appears, check that the UV lamp is installed
properly and that it is turned on. Repeat the wavelength
calibration.
Code 3154 Signal overload detected. • Perform an autozero.
• Install another flow cell (see Removing the Flow Cell
( } page 134) / Installing a Flow Cell ( } page 62) ).
• Perform autozero.
• Reinstall the previously installed flow cell.
• Perform autozero.
• When the message appears again, turn off the module. Wait for
5 seconds and turn on the module again.