7
•
Maintenance and Service
Page 132
Diode Array/Multiple-Wavelength Detectors (VC-D11, VC-D12, VF-D11)
Operating Manual
7.7.2
Preparing the Flow Cell for Storage
When
Before the flow cell is stored in its packaging or in the device if one of
the following applies:
•
Flushing the flow cell with highly pure isopropanol through the
system is not possible
•
It is shipped
Parts required
•
Flushing and injection kit for flow cells, including a syringe and a
Viper adapter
•
Highly pure solvent that is miscible with isopropanol, such as LC/MS-
grade acetonitrile or methanol
•
Highly pure isopropanol, such as LC/MS-grade isopropanol
•
Plugs for the flow cell inlet and outlet (supplied with the flow cell)
Preparations
1. Remove buffer salts by flushing the flow cell with a mixture of 50%
solvent and 50% water.
2. Flush the flow cell with a highly pure solvent that is miscible with
isopropanol, such as LC/MS-grade acetonitrile or methanol.
3. Make sure that residual sample components, impurities from the
column, aggressive solvents or eluates with salt contents are
completely flushed out of the flow cell.
4. Turn off the UV lamp.
5. Stop the pump flow to the flow cell.
Follow these steps
1. Unpack the components from the flushing and injection kit. Remove
the protection cap from the Viper fitting. Keep the packaging.
2. Screw the threaded end of the flushing adapter to the syringe.
3. Draw up highly pure isopropanol with the syringe through the Viper
adapter, for example 1.0 mL.
4. Disconnect the capillary from the flow cell inlet.
5. Connect the Viper adapter to the flow cell inlet.