5
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Installation
Page 68
Diode Array/Multiple-Wavelength Detectors (VC-D11, VC-D12, VF-D11)
Operating Manual
NOTICE
Flow cells are sensitive to contamination, clogging and high
backpressures. If the pressure exceeds the upper limit, the flow cell may
be permanently damaged. Observe the following notes when connecting
the flow cell to the system flow path:
•
Backpressures that exceed the specified maximum pressure limit of
the flow cell can destroy the flow cell. Never expose the flow cell to
excessive backpressure. When connecting a component in the flow
path after the flow cell, observe the specified backpressure for the
flow cell.
•
Pressure spikes exceeding the pressure specifications of the flow cell
can destroy the flow cell. When installing switching valves or fraction
collectors downstream of the flow cell, make sure that the resulting
pressure spikes do not exceed the pressure specifications of the flow
cell.
•
Avoid clogging of the flow cell or waste line.
•
Improperly set up flow connections can lead to leaks on the flow
cell. Do not invert the flow cell inlet and outlet.
•
Connecting dirty inlet capillaries can lead to leakages and can
damage the flow cell. Use only inlet capillaries which are clean,
which were either shipped with the flow cell (standard
biocompatible flow cells) or with the detector (all other flow cells)
and which have been properly protected by their cap before.
•
Connecting the wrong waste line can lead to leakages. Use only
those waste lines that Thermo Fisher Scientific recommends for your
flow cell (see
).
•
Connect the waste line to the flow cell only as described in the
manual.
•
Do not discharge waste from the flow cell through the open leakage
drain system of the Vanquish system.
•
Dirty components can contaminate the chromatography system.
Contamination leads to poor performance of the modules and entire
system or can even cause damage to the modules and system.
Therefore:
¨
Always wear appropriate gloves.
¨
Place the components only on a clean, lint-free surface.
¨
Keep your tools clean.
¨
Use only lint-free cloth for cleaning.