6
•
Operation
Diode Array/Multiple-Wavelength Detectors (VC-D11, VC-D12, VF-D11)
Operating Manual
Page 89
System Equilibration
System equilibration should include the following operations:
•
Purging the pump (
all
channels, including those not used for the
application)
•
Flushing the entire chromatography system with the starting solvent
to rinse out any solvent from a previous analysis run
•
Warming up (or cooling down) all temperature-controlled devices in
the system to the starting temperature. Temperature-controlled
devices can be, for example
¨
Column compartment and post-column cooler
¨
Sample compartment thermostatting in the autosampler
¨
Flow cell in a fluorescence detector
¨
Evaporation tube in a charged aerosol detector
•
Turning on the lamp (or lamps) in the UV/VIS detector
•
Monitoring the pump pressure and pressure ripple and checking
that the pressure is stable and the ripple within reasonable limits for
the application
•
Monitoring the detector signal and checking whether the detector
signal is stable so that the drift and signal noise are within
reasonable limits for the application
•
Performing an autozero of the detector baseline
TIP
The Chromeleon software supports procedures for automatically
starting a chromatography system in the software (
Smart Startup
). The
startup procedure includes the operations for system equilibration. For
details, refer to the
Chromeleon Help
.