Thermo NanoDrop One, User Manual, Page: 49 / 312

Measure Oligo DNA or Oligo RNA
Thermo Scientific NanoDrop One User Guide 43
Related Topics
• Best Practices for Nucleic Acid Measurements
 To measure an oligonucleotide sample
1. From the Home screen, select the Nucleic Acids tab
and tap either Oligo DNA or Oligo RNA , as needed.
2. Specify the Oligo base sequence and a baseline
correction if desired.
3. Pipette 1–2 μL blanking solution onto the lower
pedestal and lower the arm, or insert the blanking
cuvette into the cuvette holder.
Tip : If using a cuvette, make sure to align the cuvette
light path with the instrument light path.
4. Tap Blank and wait for the measurement to
complete.
Tip : If Auto-Blank is On, the blank measurement
starts automatically after you lower the arm. (This
option is not available for cuvette measurements.)
5. Lift the arm and clean both pedestals with a new
laboratory wipe, or remove the blanking cuvette.
6. Pipette 1-2 μL sample solution onto the pedestal and
lower the arm, or insert the sample cuvette into the
cuvette holder.
7. Start the sample measurement:
– Pedestal: If Auto-Measure is On, lower arm; if
Auto-Measure is off, lower arm and tap Measure .
– Cuvette: Tap Measure.
When the sample measurement is completed, the
spectrum and reported values are displayed (see the
next section).
8. When you are finished measuring samples, tap
End Experiment .
9. Lift the arm and clean both pedestals with a new
wipe, or remove the sample cuvette.
Example Oligo DNA spectrum