Thermo NanoDrop One, User Manual, Page: 208 / 312

3
Learning Center
Prepare Samples and Blanks
208 NanoDrop One User Guide Thermo Scientific
Related Topics
• Absorbance Detection Limits
1. From the Home screen, select an application tab such
as Nucleic Acids and tap an application name such as
dsDNA or RNA.
2. Lift the instrument arm and clean the upper and
lower pedestals with new laboratory wipe.
3. Measure a water blank:
– Pipette exactly 1 μL deionized water (DI H
2
O)
onto the lower pedestal and lower the arm.
– Tap Blank and wait for the measurement to
complete.
– Lift the arm and clean both pedestals with new
laboratory wipe.
4. Measure the buffer solution:
– Pipette 1-2 μL buffer solution onto the pedestal
and lower the arm.
– Start the sample measurement:
– if Auto-Measure is On, lower arm
– if Auto-Measure is off, lower arm and tap
Measure
– Wait for measurement to complete.
The resulting spectrum should vary no more than
0.04 A from the baseline at the analysis wavelength
(260 nm for nucleic acids; 280 nm for proteins).
If your spectrum does not meet these criteria, repeat
steps 2–4.
If spectrum is still outside specifications, see Solutions
for Blanking Problems .
5. When you are finished with the blanking cycle, tap
End Experiment .
6. Lift the arm and clean both pedestals with a new
wipe.
Example spectrum of buffer suitable
for Protein A280 protein quantification
Example spectrum of buffer unsuitable
for Protein A280 protein quantification