Menus, Tabs and Parameters
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Thermo Fisher Scientific
Thermo Scientific Wellwash Versa User Manual
79
●
Soak time (mm:ss)
– Define the time period between a wash cycle
in microplate wash protocols. The minimum value is 0 s and the
maximum value 60 min in increments of 1 s. The default value is
00:01
.
●
Shake speed
–Define the shake speed Off, Low, Medium, or High.
The default is
Off
. The shake speed setting is only effective when the
soak time is >
00:00
.
Caution
Ensure that the liquid does not spill at the shake speed and
volume settings in use.
▲
Caution
The instrument is not intended for shaking purposes only. A
separate microplate shaker is available if needed (e.g. iEMS
Incubator/Shaker). Refer to
www.thermoscientific.com
.
▲
The Prime step is performed to fill the liquid tubing completely from
the selectable buffer bottle intake tube to the wash head tips. A protocol
should have a Prime step before each buffer is used to ensure that the
liquid tubing is filled correctly with the desired liquid before performing
any Wash or Dispense steps.
The default priming volume is
30 ml
in order to fill the tubing properly.
The dead volume of the liquid tubing is 20 ml (Figure 5-34).
Note
Avoid unnecessary priming with air because it may damage the
pump.
▲
The
Prime
step has the following parameters:
●
Buffer source
– Define the buffer source channel A, B, or Rinse.
The default is
A
.
●
Prime volume (ml)
– The prime volume ranges from 5 ml to
100 ml in 5 ml increments. The default is
30 ml
.
Priming is carried out in the priming position. Aspiration is on while
priming. At the end, the priming vessel is emptied and the wash head
returns to the home position.
Prime
