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Running Protocols

Loading the plate

Thermo Scientific Wellwash Versa User Manual

Thermo Fisher Scientific

The wash head defines the plate type and orientation (Table 5–3 and
Figure 5–31 and Figure 5–32).

Table 5–3.

Wash head vs. plate and orientation

Wash head

Plate

Orientation

nx8 96-well

plate

Landscape

2x8 (cell wash)

96-well plate

Landscape

nx12 96-well

plate

Portrait

1x16 384-well

plate

Landscape

Figure 5–31.

2x8 orientation (A) and 1x16 orientation (B)

Figure 5–32.

2x12 orientation

Caution

Ensure that the microplate is correctly oriented to match the

wash head (Table 5–3).

▲

Caution

Ensure that the strips on a strip plate are pressed flat on the

frame. Misaligned strips may increase the residual volume or limit plate
carrier movement.

▲

Loading the

plate

Summary of Contents for Wellwash Versa

Page 1: ...Thermo Scientific Wellwash Versa User Manual Rev 1 3 ...

Page 2: ......

Page 3: ...Thermo Scientific Wellwash Versa User Manual Rev 1 3 Cat No N11166 ...

Page 4: ...ion to ensure accuracy Thermo Fisher Scientific assumes no liability for any errors or omissions nor for any damages resulting from the application or use of this information This manual supersedes all previous editions Remarks on screenshots and pictures The version number displayed in screenshots may not always be the one of the currently released version Screenshots and pictures are only replac...

Page 5: ...is user manual also describes all the features and specifications of the Wellwash Versa instrument as well as ordering information Read the manual in its entirety before operating the instrument Keep the user manual on the CD provided for future reference The user manual is an important part of the instrument and should be readily available during use of the instrument Keep the user manual togethe...

Page 6: ...resence of hazards as indicated The following symbols and markings appear on the type label and the instrument itself Power ON Power OFF Serial number Catalog number Date of manufacture Consult instructions for use WEEE symbol This product is required to comply with the European Union s Waste Electrical Electronic Equipment WEEE Directive 2012 19 EC Safety symbols and markings Safety symbols and m...

Page 7: ...ual Warning Risk of electric shock Warning Biohazard risk Warning Risk of injury to the user s Caution Risk of damage to the instrument other equipment or loss of performance or function in a specific application Note Marks a hint important information that is useful in the optimum operation of the system or an item of interest Warning and other markings used in the documentation ...

Page 8: ...About This User Manual Safety symbols and markings 6 Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific ...

Page 9: ...ng the transport lock 16 Installing the priming vessel 17 Installing the wash head s 18 Installing the cell wash head 21 Liquid bottles and channels 22 Connection diagram 23 Installing the liquid bottles 24 Foaming 27 Connecting the power supply cable 27 Connecting to a computer 28 Warnings and cautions 28 Electrical 28 Defects and abnormal stresses 29 Operating precautions and limitations before ...

Page 10: ...ystem 47 Strip selection 48 Strip selection with the number keys 49 Strip selection with the Layout row 50 Starting a run 51 Canceling a run 51 Rinsing the liquid system 51 Menus Tabs and Parameters 53 Display and keys for navigating and editing 53 Menus 55 Main menu 55 Liquid level detection 56 Protocol 56 Creating a protocol 57 Creating a protocol in an optional way 60 Adding new steps to protoc...

Page 11: ...ol 104 Exporting a protocol as text file 105 Deleting 107 Deleting a protocol 107 Shutdown 109 Shutting down 109 Emergency Situations 111 Handling abnormal situations 111 Regular and preventative maintenance 113 Maintenance 113 Maintenance checklist 113 Instrument care 114 Cleaning the instrument 115 Salt deposit 115 Cleaning the priming vessel 115 Cleaning the plate carrier 116 Cleaning liquid bo...

Page 12: ...pack for service 125 Disposal of the instrument 126 Technical Specifications 129 General specifications 129 Performance specifications 130 Safety specifications 131 In conformity with the requirements 131 Troubleshooting Guide 133 Error and warning codes 133 USB memory device 134 Troubleshooting guide 135 Ordering Information 137 Wellwash Versa 137 List of spare parts and accessories 137 System Lo...

Page 13: ...mat Additionally a 2x8 cell wash head is available for washing of cells in 96 well plates The instrument allows shaking The instrument is controlled through the built in graphical user interface and keypad In addition the Wellwash Versa can be connected to plate handling devices The Wellwash Versa can be used to wash and prepare plates for a variety of test routines mainly in enzyme linked immunos...

Page 14: ...ntation The instrument is equipped with three standard buffer bottles and one waste liquid bottle each with a cap tubing and a liquid level sensor The instrument has a color display with an intuitive graphical user interface and keypad for programming and controlling the instrument The Wellwash Versa comes with a 1x8 2x8 1x12 or 2x12 wash head The plate carrier places the microplate accurately und...

Page 15: ...er Principle of operation Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual 13 Figure 1 2 Wash heads Figure 1 3 Principle of washing step Normal Figure 1 4 Principle of washing step Sweep2 Figure 1 5 Principle of washing step Sweep3 ...

Page 16: ...Introduction to the Wellwash Versa Microplate Washer Principle of operation 14 Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific ...

Page 17: ...s or parts other than those specifically designated in the instructions Doing so may cause misalignment and will invalidate the instrument warranty Retain the original packaging for future transportation The packaging is designed to assure safe transport and minimize transit damage Using other packaging materials may invalidate the warranty Also retain all instrument related documentation provided...

Page 18: ... potentially damaging liquids or gases are present This section describes the procedures that must be carried out before instrument operation Warning All parts of the instrument that come into contact with potentially infectious materials must be treated as potentially infectious areas It is advisable to adhere to applicable safety precautions such as the wearing of disposable powder free gloves s...

Page 19: ...ort lock and transport lock tag 2 Unfasten the transport lock screw with the Allen key supplied Figure 2 7 Figure 2 7 Unfastening the transport lock 3 Keep the transport lock and tag for future relocation or transportation of the instrument The instrument comes with the priming vessel installed The priming vessel is correctly installed if it stays in place and does not move up If the priming vesse...

Page 20: ...l number according to which it has been calibrated at the factory Optional wash heads should be calibrated by the user prior to use Warning The wash heads may be contaminated after the instrument has been used Warning Failing to connect the dispensing or aspiration tube may cause spillage Caution Only use wash heads that have an identification label Note Always wear disposable powder free gloves w...

Page 21: ...for easier recognition Figure 2 9 Optional wash heads A as well as aspiration and dispensing tubes B Note four tubes two blue tubes on the left hand side and two clear tubes on the right hand side Figure 2 10 Fitting the wash heads to the connectors 3 Fit the wash heads next to each other onto the wash head arm with the tips pointing downwards and ensure that the wash heads move freely up and down...

Page 22: ...nly inserted Figure 2 12 Figure 2 12 2x8 wash heads evenly inserted 5 Ensure that the wash heads are in the correct order Figure 2 13 6 If only one wash head 1x8 or 1x12 is used place the blank wash head onto the left position of the wash head arm Caution If you use the device with a blank wash head the dry tubes will cause the pump to operate more loudly It is recommended to prime both channels t...

Page 23: ...head on page 97 8 If you change the wash head to another type first change the wash head and then calibrate the wash head Install the optional 2x8 96 cell wash wash head properly onto the wash head arm Figure 2 14 Refer to Installing the wash head s on page 18 Note Check that the brown adapters of the 2x8 cell wash head are inserted Figure 2 14 Note When you replace the cell wash head adapters Fig...

Page 24: ... for Rinse and W for Waste Channel R is primarily intended for rinsing and is connected to the RINSE button as the only channel The Rinse channel can also be used in wash protocols as the buffer source The rinse solution is normally deionized distilled water The liquid bottles are named and the tubing is color coded to correspond to the correct buffer source A blue B yellow R green and W colorless...

Page 25: ... the float on the buffer bottle sensor Smaller bottles can also be used with an optional bottle holder Cat No N10820 Caution If a smaller size buffer bottle is used with the bottle stand the remaining volume in the bottle after the liquid level sensor indicates empty may be smaller than the nominal 290 ml Caution Before disconnecting the tubing prime the liquid system with air to avoid liquid spil...

Page 26: ... Wellwash Versa connection diagram 1 Fit the liquid bottle tubing to the corresponding color coded tube connectors on the left side panel of the instrument Figure 2 18 The connectors are from left to right W colorless Waste R green Rinse B yellow Buffer B and A blue Buffer A Installing the liquid bottles ...

Page 27: ...le tubing 2 Connect the liquid sensor cables into the appropriate color coded sockets on the left side panel of the instrument Figure 2 19 The sockets are from top to bottom W Waste R Rinse B Buffer B and A Buffer A Figure 2 19 Connecting the liquid sensor cable All the liquid bottles are connected to the instrument in Figure 2 20 ...

Page 28: ...h as disposable gloves a laboratory coat and safety glasses when emptying or handling the waste bottle Caution Always ensure that the liquid bottles are attached properly Always connect the bottle cap to the correct bottle and to the correct connector on the left side of the instrument Otherwise the wash performance can be seriously affected Caution Ensure that the intake tube reaches fully down t...

Page 29: ...y increasing the concentration of anti foaming agent in the waste bottle 4 Carefully swirl the waste bottle from time to time to improve mixing between the foam layer and anti foaming agent To connect the power supply cable Warning Do not operate your instrument from a power outlet that has no ground connection Do not use a power supply cable other than the Thermo Scientific power supply cable des...

Page 30: ...h the unit is always used If a correct type of mains cable is not provided use only cables certified by the local authorities The power plug should only be inserted into a socket outlet with a protective ground contact Do not use an extension cable without a protective ground wire Warning Only authorized technical service personnel are allowed to open the instrument The same precautions applicable...

Page 31: ...ts entirety as it contains information necessary to ensure safe operation 2 Always ensure that the electrical supply in the laboratory conforms to that specified on the type label at the rear of the instrument 3 Ensure that the bottle and wash head tubing are properly fitted Ensure that the intake tube reaches completely down to the bottom of the liquid bottle so that no red tubing is visible Chec...

Page 32: ...tly If there are strips missing on a plate ensure that they are not selected for processing If there are unused wells on the strip it is recommended to fill unused wells with the same amount of deionized distilled water as in the used wells Before switching the instrument on Move the wash head arm up and down and the plate carrier back and forth to ensure that they move freely Figure 2 22 Lift the...

Page 33: ...age or the local date time refer to Settings menu on page 81 Caution Do not switch the power off nor plug unplug the USB memory device during Performing self diagnostics Caution Do not touch the wash head or plate carrier when the instrument is in use Note Ensure that the wash heads are installed Before putting the instrument into use perform the following operational check For more information re...

Page 34: ...ripping For more information refer to Priming the system on page 47 Check that the wash head configuration in the Settings menu corresponds to the installed wash head The information is used to check for protocol wash head mismatches Refer to Wash head configuration on page 84 Run a protocol such as one of the demo protocols supplied with the instrument The protocol should have an aspirate dispens...

Page 35: ...instrument The front view of the Wellwash Versa instrument is shown in Figure 3 24 Figure 3 24 Wellwash Versa front view with accessories The back view of the Wellwash Versa instrument is shown in Figure 3 25 Instrument views Wash heads on wash head arm Priming vessel Keypad Display Plate carrier Transparent aerosol cover Liquid bottles Plate clamp Bottle stand option ...

Page 36: ...3 26 Wellwash Versa side views Connectors for liquid bottle tubes from left to right W colorless Waste R green Rinse B yellow Buffer B and A blue Buffer A Mains I O switch Power supply connector USB port for PC USB port for memory device Mains switch USB port for memory device Connectors for liquid sensor cables from top to bottom W colorless Waste R green Rinse B yellow Buffer B and A blue Buffer...

Page 37: ...can check the functionality of the USB memory device by inserting it into the USB port for memory device Figure 3 25 The WELLWASH folder will be created The instrument is equipped with a USB port for an external PC connection The connection is used for remote control when connected for example to a robotic system and for service use A separate manual Thermo Scientific Wellwash Versa Remote Control...

Page 38: ...ad alternatives Figure 3 28 and their configurations Figure 3 29 are shown below Figure 3 29 shows how to set up wash heads for different configurations Warning Do not remove the wash head plugs when in use Caution Only use wash heads that have an identification label Figure 3 28 Wellwash Versa wash head models Wash heads ...

Page 39: ...o improve washing efficiency The linear shaker operates at three different speeds Table 3 1 Table 3 1 Shaking speeds Speed designation Speed Low 5 Hz amplitude 2 5 mm Medium 10 Hz amplitude 1 5 mm High 15 Hz amplitude 1 mm Caution The instrument is not intended for shaking purposes only A separate microplate shaker is available if needed e g Thermo Scientific iEMS Incubator Shaker Refer to www the...

Page 40: ...Wellwash Versa Main Parts Shaker 38 Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific ...

Page 41: ...epending on the level in the user interface The active row is colored blue Use the Left Right Up and Down arrow keys to navigate You can speed up the selection by holding down the arrow key Use the OK button to select edit or accept the highlighted item Display and keys for navigating and editing Menu bar Active row blue Info text bar Function keys Numeric keys with letters and signs also used for...

Page 42: ... New Open Save Save As Quick Key Export Import and Delete among others Use the HELP key for more detailed instructions about the context Press the PRIME button to prime the instrument Press the RINSE button to rinse the instrument Press the START button to start the execution of the currently selected protocol Press the STOP button to terminate the active protocol execution Pressing the button als...

Page 43: ...he Main menu The Main menu contains the Protocol Head Plate Well Steps and Layout rows and their parameters The clock on the menu bar shows the local time Note that the blue clock icon appears on the menu bar when Autorinse or Autoprime is active An asterisk in front of a protocol name means that the protocol has not yet been saved A yellow caution symbol on the Head Plate row means that the curre...

Page 44: ...page 83 Liquid level detection LLD Liquids A B and Rinse icon is green when the bottle is filled with liquid icon is red with an exclamation mark when the bottle is nearly empty or disconnected The warning is activated when there is approximately 290 ml in the 2 liter wash bottle 580 ml in the 4 litter wash bottle icon is empty with a cross when the LLD is disabled Waste icon is red with an exclam...

Page 45: ...ble above the cap and fully immersed in the buffer bottle 2 Select a protocol Refer to Starting ready made protocol on page 44 The selected protocol name is shown on the Protocol row in the Main menu 3 Ensure that the protocol parameters are correct 4 Insert the 96 or 384 well plate to be washed onto the plate carrier and ensure that the microplate is correctly oriented according to the wash head ...

Page 46: ...on matches the physical strips on the plate Note Do not remove the plate before the end of the protocol run Note It is recommended to shut down the Wellwash Versa at the end of the day Refer to Shutting down on page 109 1 Disconnect the liquid bottles and fill or empty them if needed Refer to Liquid level detection on page 42 2 Unscrew the liquid bottle cap and fill with a suitable buffer solution...

Page 47: ...1 F3 keys You may change these protocols from Protocol OK FILE Quick Key Set Clear F1 F3 1 Press the OK button on the Protocol row in the Main menu OR Press the FILE key in the Main menu and select Open using the Down arrow key and then press the OK button 2 Select the ready made protocol and press the OK button Starting a ready made protocol with the quick keys Starting a ready made protocol from...

Page 48: ...nx8 96 well plate Landscape 2x8 cell wash 96 well plate Landscape nx12 96 well plate Portrait 1x16 384 well plate Landscape Figure 5 31 2x8 orientation A and 1x16 orientation B Figure 5 32 2x12 orientation Caution Ensure that the microplate is correctly oriented to match the wash head Table 5 3 Caution Ensure that the strips on a strip plate are pressed flat on the frame Misaligned strips may incr...

Page 49: ...quid It is recommended to use deionized distilled water in unused wells on the strip Press the PRIME button to fill the liquid tubing completely from the selectable buffer bottle intake tube to the wash head tips If you prime for the first time fill the buffer bottle completely to ensure proper priming The default priming volume is 30 ml to fill the liquid tubing properly On subsequent priming occ...

Page 50: ...ssible air leaks Note If the instrument has not been used for a longer time you might need to perform the Boost prime option Refer to Service on page 102 Note In case of two buffer protocols both the buffer channels have to be primed before the protocol is started If channels are switched within the same protocol add a Prime step to the protocol to ensure that liquid in the tubing is replaced comp...

Page 51: ...to select two strips at a time 2x12 wash head strips 1 to 8 press number keys 1 8 to select two strips at a time All columns strips Double press the 8 or 12 key to select all columns strips when the selection is empty Select or unselect a column strip Press the corresponding number key Range of strips First select the starting strip and then double press the key corresponding to the final strip in...

Page 52: ...utton Select unselect columns strips by pressing the OK button and move the cursor using the Left or Right arrow key All selections are cleared with the C key Columns strips 1 12 are edited if an 8 way wash head is used or columns strips 1 8 if a 12 way wash head is used The selected columns strips are highlighted with red color Strip selection with the Layout row ...

Page 53: ...er at the end of the batch To cancel a run Press the STOP button to cancel a run Note It is not possible to stop the run when a user input is required as a response to a warning or error message The RINSE button starts a sequence where the rinse liquid is pumped through the liquid system for a short period of time and left in the tubing The rinse solution is normally deionized distilled water The ...

Page 54: ...Running Protocols Rinsing the liquid system 52 Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific ...

Page 55: ...epending on the level in the user interface The active row is colored blue Use the Left Right Up and Down arrow keys to navigate You can speed up the selection by holding down the arrow key Use the OK button to select edit or accept the highlighted item Display and keys for navigating and editing Menu bar Active row blue Info text bar Function keys Numeric keys with letters and signs also used for...

Page 56: ...ol New Open Save Save As Quick Key Export Import and Delete among others Use the HELP key for more detailed instructions about the context Press the PRIME button to prime the instrument Press the RINSE button to rinse the instrument Press the START button to start the execution of the currently selected protocol Press the STOP button to terminate the active protocol execution Pressing the button a...

Page 57: ...the Main menu The Main menu contains the Protocol Head Plate Well Steps and Layout rows and their parameters The clock on the menu bar shows the local time Note that the blue clock icon appears on the menu bar when Autorinse or Autoprime is active An asterisk in front of a protocol name means that the protocol has not yet been saved A yellow caution symbol on the Head Plate row means that the curr...

Page 58: ...n the 4 litter wash bottle icon is empty with a cross when the LLD is disabled Waste icon is red with an exclamation mark when the bottle is full or disconnected icon is green when the bottle is not yet full icon is empty with a cross when the LLD is disabled The Protocol row in the Main menu shows the name of the active protocol You can open another protocol by pressing the OK button on the Proto...

Page 59: ...ocols and Creating a protocol on page 57 1 Press the FILE key in the Main menu to create a new protocol Refer to Protocol on page 56 2 Select New and press the OK button 3 Select the Head Plate row Refer to Head Plate on page 63 4 Select the wash head and plate If the selected wash head is different from the one that is set in the Settings menu you must change the wash head in the Settings menu be...

Page 60: ... to default values Soak and Pause steps are not affected 5 Select Well The Well type window opens Refer to Well on page 64 6 Select the well bottom shape of the plate or strips 7 Select Well offset to adjust the well offset value For more information on the Preview function refer to Well on page 64 Note Well offset has an effect on the residual volume 8 Select the Steps row in the Main menu Refer ...

Page 61: ... It is possible to create several steps in the step list and process the steps in the defined sequence For more information on the step parameters refer to Steps on page 66 11 Press the FILE key in the Main menu Note that the strip selection is not saved with the protocol Use the Layout step if you want to save the strip selection 12 Select Save As The Save Protocol As dialog opens ...

Page 62: ...s now created Note You cannot use the protocol name Untitled Note You cannot save a protocol with a name already in use You can also create a protocol using the Protocol window 1 Select the Protocol row in the Main menu and press the OK button 2 Select the Create a new protocol row and press the OK button 3 Enter a name for the protocol and press the OK button Creating a protocol in an optional wa...

Page 63: ...In the Main menu set the desired protocol parameters and steps 5 When ready press the FILE key and select Save to save your protocol To add a new step to a protocol 1 Select the Steps row in the Main menu and press the OK button 2 Press the F3 New step key to open the step list box Adding new steps to protocols ...

Page 64: ...using the Up or Down arrow key and then press the F3 Insert key 5 Press the OK button to edit the new step For more information on steps refer to Steps on page 66 6 Save the changes you make in each step using the File Save functions 7 Press the F3 New step key to add further new steps to the protocol When there are more than five steps blue arrows appear at the bottom and or at the top of the dis...

Page 65: ... the OK button to confirm the deletion You can select the wash head and plate format on the Head Plate row in the Main menu Caution If you edit an existing protocol and change the wash head the wash head related step parameters of the protocol e g the Wash step are automatically reset to default values Soak and Pause steps are not affected Deleting steps from protocols Head Plate ...

Page 66: ...t the wash head and plate and press the OK button Refer to Wash head configuration on page 84 and Creating a protocol on page 57 Note Calibrate the wash head s if not already calibrated You can select the well shape and well offset on the Well row in the Main menu If you change the well shape check the step parameters afterwards When you press the OK button the Well type window appears The followi...

Page 67: ... plates check that the offset limit is not too big to prevent dispensing outside the wells Use the Up and Down arrow keys to adjust the value Press the F3 Preview key to move the plate carrier and align the wash head over the first column of a plate Then press the Up or Down arrow key to move the plate carrier in steps of 0 1 mm Warning Well offset has an effect on the residual volume in the well ...

Page 68: ...ght 14 5 mm 0 2 mm 14 5 mm 0 1 mm 14 0 mm 0 3 mm 14 5 mm 0 1 mm Well to well distance 9 0 mm 384 wells 464718 Nunc F384 Flat 2 7 mm Max overall plate height 14 4 mm 0 25 mm Well to well distance 4 5 mm Thermo Scientific Nunc Not valid for a 96 well plate with a 2x8 96 cell wash wash head You can create or edit the wash protocol in the Main menu It is recommended to test the optimal parameters for ...

Page 69: ... of Wash step Sweep2 Figure 6 39 Principle of Wash step Sweep3 Figure 6 40 Dispense heights The wash step is the step needed in most wash protocols The well is first emptied then filled with buffer and finally emptied or left full Wash Dispense height end Dispense height start Well bottom Plate bottom 0 mm Dispensing Aspirating Aspirating Aspirating Dispensing Dispensing ...

Page 70: ...value is 300 μl For a 384 well plate the range is 20 μl to 300 μl in 10 μl increments The default value is 100 μl Note It is possible to dispense more than the well volume and the excess liquid is aspirated as the aspiration is always on If a so called overflow volume is selected where the dispensing volume is greater than the well volume pay attention to the dispense height Check that the wash he...

Page 71: ...ss each selected strip This selection is recommended when the timing for washing strips is critical You can maintain the time between cycles constant with this option It is recommended to carry out a dummy run before the actual samples Refer to Figure 6 37 Aspirate mode Define the aspiration mode Normal Sweep 2 or Sweep 3 Sweep is allowed for 96 well plates with flat wells The default value depend...

Page 72: ...0 mm to 17 mm in 0 1 mm increments The default is 6 0 mm Ensure that the value fits the plate in use Because dispensing starts from a lower level in a well and ends at a higher level the start height should be lower than or equal to the end height Refer to Figure 6 40 Dispense height end The dispense height end ranges from 0 mm to 17 mm in 0 1 mm increments The default is 14 4 mm Refer to Figure 6...

Page 73: ...will act as a shield to prevent trauma to the cell layer during the subsequent fluid dispense It also has the added advantage of maintaining cell hydration The aspirate height has to be set higher than the cell layer Avoiding aspiration delays is also critical There is a significant amount of vortex turbulence in the region immediately surrounding the aspiration tips which has the potential to be ...

Page 74: ...olume settings in use Wash mode Define the wash mode The default is Plate mode Plate All selected strips are processed before the next cycle Strip Each selected strip is processed the number of cycles defined before moving to the next strip Aspirate height The aspirate height ranges from 0 mm to 14 mm in 0 1 mm increments The default height varies according to the plate type and well shape Table 6...

Page 75: ...mm in 0 1 mm increments The default is 12 0 mm Refer to dispense height start above Refer to Figure 6 40 Note Because aspiration is on during dispensing the end volume in the well depends on the dispense height If the liquid level in the well during dispensing reaches the wash head tip then excess liquid is aspirated Note It is possible to dispense more than the well volume and the excess liquid i...

Page 76: ... Aspirate mode Define the aspirate mode Normal Sweep 2 or Sweep 3 The default is Normal Sweep is allowed for 96 well plates with flat wells Refer to Figure 6 38 Normal There is one aspirate position at the center of the well Sweep2 Aspirates in two different positions across the bottom of the well Sweep3 Aspirates in three different positions across the bottom of the well Aspirate height mm The as...

Page 77: ...e first protocol run The wash head stays above unused strips for as long as it takes to process each selected strip This selection is recommended when the timing for washing strips is critical You can maintain the time between cycles constant with this option A Cell aspirate step removes liquid from the wells Refer to Cell wash on page 71 The Cell aspirate step has the following parameters Aspirat...

Page 78: ...unused strips for as long as it takes to process each selected strip This selection is recommended when the timing for washing strips is critical You can maintain the time between cycles constant with this option A Dispense step fills the wells with liquid The Dispense step has the following parameters Buffer source Define the buffer source channel A B or Rinse The default is A Dispense volume μl ...

Page 79: ...sh head stays above unused strips for as long as it takes to process each selected strip This selection is recommended when the timing for washing strips is critical You can maintain the time between cycles constant with this option A Cell dispense step fills the wells with liquid dropwise Refer to Cell wash on page 71 The Cell dispense step has the following parameters Buffer source Define the bu...

Page 80: ...is 0 5 mm Dispense tip touch The dispense tip touch movement is used to remove the last droplet from the wash head tips after the dispensing action The dispense tip touch ranges from 0 0 mm to 1 5 mm in 0 1 mm increments The default is 1 0 mm Strip over mode Define the strip over function The default is No No All selected strips are processed as fast as possible Yes If Yes is selected unused strip...

Page 81: ...om The Prime step is performed to fill the liquid tubing completely from the selectable buffer bottle intake tube to the wash head tips A protocol should have a Prime step before each buffer is used to ensure that the liquid tubing is filled correctly with the desired liquid before performing any Wash or Dispense steps The default priming volume is 30 ml in order to fill the tubing properly The de...

Page 82: ...ee second alarm is sounded The Layout step is used to predefine the strip selection for a protocol This step determines the area on which the next steps are processed The layout is landscape when a 1x8 2x8 or 1x16 wash head is selected and portrait for a 1x12 or 2x12 wash head The strips are numbered 1 12 if an nx8 wash head is used and marked A H for an nx12 wash head The strips are numbered 1 24...

Page 83: ...ed If the Layout step is used it is recommended to use the step as the first step in the protocol If the Layout step is not the first step in the protocol the Layout row is not locked but enabled You can select strips using the row until the first Layout step in the protocol is encountered The Settings menu contains the Prime rinse parameters Sensors Wash head configuration System and Reports para...

Page 84: ... ml The rinse volume ranges from 5 ml to 100 ml in 1 ml increments The default is 50 ml Autorinse The washer can be programmed to rinse the system automatically using the rinse liquid with a selected volume At the end of the run the software starts to count down the selected time Thirty seconds before the autorinse starts the buzzer gives an audible sound and the remaining time is shown on the dis...

Page 85: ...ull Failure may result in a weakened or faulty wash result liquid spillage or biohazard Select the Sensors row in the Settings menu and press the OK button The Sensors window has the following parameters Buffer A sensor Disable or enable the buffer A sensor The default is Enabled Buffer B sensor Disable or enable the buffer B sensor The default is Enabled Buffer Rinse sensor Disable or enable the ...

Page 86: ... the OK button The Wash head configuration window has the following parameters Wash head Ensure that the installed wash head is the same as set in the Wash head configuration window Press the OK button to select the correct wash head from the list 1x8 96 1x12 96 1x16 384 2x8 96 2x12 96 2x8 96 cell wash Warning An incorrect wash head configuration can cause liquid spillage or a faulty washing perfo...

Page 87: ...m dd or mm dd yyyy The default is dd mm yyyy Time format Select the time format 12 hour or 24 hour The default is 24 hour Buzzer Disable or enable the buzzer The alternatives are All on Keyb off or All off All the warning signals are off in All off The default is Enabled and All on Language Set the preferred user interface language The default is English English 中文 Chinese Français French Deutsch ...

Page 88: ... memory device to the USB memory device port in the instrument select the desired report and press the OK button The selected report is exported to the USB memory device under an automatically generated folder named WELLWASH The reports available are Error log The error log shows the date and time of the error and the error s Status report The status report shows the following parameters report na...

Page 89: ...sinfection procedure see Cleaning Disinfection procedure on page 87 Clean wash head see Clean wash head on page 90 Soak wash head see Soak wash head on page 92 Move wash head see Move wash head on page 93 Empty priming vessel see Empty priming vessel on page 94 The procedure cleans or disinfects the instrument according to a set of actions Refer to Chapter 11 Maintenance particularly Decontaminati...

Page 90: ...ottles with cleaning reagent Press the OK button 3 Select the soak time using the Right arrow key and number keys The default is 15 min Press the OK button Priming preparing the soak and soaking take place 4 Press the F2 key to abort the soaking if you want a shorter soak time than the set soak time and to continue the procedure Priming is then carried out ...

Page 91: ...ty all bottles and fill them with deionized distilled water Press the OK button Priming preparing the soak and soaking take place The default soak time is 2 s Finally the priming vessel is emptied 6 Empty all bottles and press the OK button Priming is then carried out 7 Press the OK button when the procedure has finished ...

Page 92: ...e wash heads are dirty or clogged The instrument fills the priming vessel with a selected liquid and soaks the wash head for a selected period of time Note Use a suitable cleaning agent and repeat the Clean procedure with deionized distilled water 1 Select the Clean wash head row and press the OK button 2 Change the wash buffer to cleaning agent or deionized distilled water Press the OK button Cle...

Page 93: ...Right arrow key and number keys The default is 15 min Press the OK button Priming preparing the soak and soaking take place 5 Press the F2 key to stop the soaking if you want a shorter soak time than the set soak time and to continue the procedure Priming is then carried out 6 Press the OK button when the procedure has finished Repeat the wash head cleaning procedure with deionized distilled water...

Page 94: ...d in the solution This procedure can be used for a shorter period of time between washes using a wash buffer or to soak for an extended period for example overnight using deionized distilled water Rinse channel 1 Select the Soak wash head row using the Down arrow key and press the OK button 2 Change the wash buffer to cleaning agent or deionized distilled water if needed Press the OK button 3 Sele...

Page 95: ...inished The procedure moves the wash head down into the priming vessel to the soak position You need to add deionized distilled water or a suitable cleaning agent manually into the priming vessel This procedure can be used to leave the wash head in liquid standby or when a clogged wash head is cleaned and the instrument pump cannot be used to fill the priming vessel 1 Select the Move wash head row...

Page 96: ... and soaking take place 3 Press the F2 key to end the procedure 4 Press the OK button when the procedure has finished The procedure aspirates the priming vessel empty of liquid The procedure can be carried out for example after the Move wash head procedure Refer to Move wash head on page 93 1 Select the Empty priming vessel row using the Down arrow key and press the OK button Empty priming vessel ...

Page 97: ...ted by the user Note It is recommended to check the dispense accuracy regularly and calibrate if necessary Refer to Chapter 11 Maintenance Note After the calibration procedure verify the accuracy by dispensing the required volume onto a plate Required tools Calibrated laboratory scale with 0 0001 g resolution 4 1 96 well plates Approximately 500 ml of liquid The purpose of the calibration is to ca...

Page 98: ...lculate the dispense valve open time versus volume Note Ensure that the wash head type corresponds to the wash head setting in the Settings menu Note Before running the calibration make sure that the liquid system operates correctly for example that the liquid filters and wash head tips are clean Select the Calibration row in the Maintenance menu and press the OK button Starting the calibration ...

Page 99: ...be temporarily activated and is active until the power is switched off User The date of the last calibration is shown initially the date of the calibration in production The calibration is used for calibrating the wash head Note A new wash head of the same kind must be calibrated before use 1 Select the Calibrate current wash head row and press the OK button Active calibration Calibrate current wa...

Page 100: ...hermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific 2 Press the OK button to start the calibration of the wash head 3 Select the used channel using the Up arrow key and or press the OK button 4 The dispense pump is calibrated first ...

Page 101: ...irst tare weighed plate onto the plate carrier and press the F1 key to continue Dispensing is carried out to 6 Weigh the dispensed plate and enter the mass of the dispensed liquid in grams using the number keys 7 Insert the second tare weighed plate onto the plate carrier and press the F1 key to continue Dispensing is carried out ...

Page 102: ... in grams using the number keys 9 Press the F1 key to continue calibration OR Press the F2 key to cancel calibration OR Press the F3 key to repeat calibration when the software informs that the Delta or accuracy are not acceptable The accuracy of the first plate must be 50 and that of the second plate must be 5 10 Continue dispensing and weighing the third and fourth plate in the same way ...

Page 103: ...y are not acceptable The accuracy must be 2 5 3 5 x that of 100 μl 12 Calibration is now complete Press the F1 key to accept the new calibration Note Calibration for the wash head is saved and it becomes the active calibration 13 Press the OK button when the procedure has finished 14 Verify the new performance by dispensing a plate for example with 300 μl Tare weigh the plate dispense and measure ...

Page 104: ...Use only 2x8 or 2x12 wash heads Do not use other wash heads for boost priming Note The liquid level sensors must be enabled Settings Sensors to allow boost priming Refer to Sensors on page 83 Note Attempt the Prime function before performing the Boost prime option Refer to Priming the system on page 47 Start the Boost prime option by pressing the OK button The pump speed is increased in steps to t...

Page 105: ...ols to or from one instrument to another of the same type To export a protocol from the instrument to a USB memory device 1 Insert the USB memory device Figure 7 41 Figure 7 41 USB memory device inserted 2 Select the Protocol row in the Main menu and press the OK button The list of protocols appears 3 Select the protocol you want to export Exporting a protocol ...

Page 106: ...ion of an exported protocol is PRO The file is in binary format You can export protocols one at a time Ensure that exporting overwrites the protocol if it already exists on the USB memory device When importing ensure that the same protocol name is not used To import a protocol from a USB memory device to the instrument 1 Insert the USB memory device Figure 7 41 2 Select the Protocol row in the Mai...

Page 107: ...t and press the OK button You can import protocols one at a time The protocol is imported to the instrument and it is available in the list of protocols It is also possible to import protocols from the Wellwash instrument You can export a protocol as a text file to a USB memory device The text file has information about the protocol such as the name the steps and their parameters modification date...

Page 108: ...in the Main menu and press the OK button The list of protocols appears 3 Select the protocol you want to export 4 Press the FILE key to open the File menu 5 Select ASCII export and press the OK button The protocol is exported to the USB memory device The ASCII exported file name is the same as that of the binary file with the TXT extension You can open the file with a text editor print it or save ...

Page 109: ...apter 8 Deleting To delete a protocol 1 Select the Protocol row in the Main menu and press the OK button The list of protocols appears 2 Select the protocol you want to delete 3 Press the FILE key to open the File menu 4 Select Delete and press the OK button Deleting a protocol ...

Page 110: ...Deleting Deleting a protocol 108 Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific 5 Select Yes and press the OK button to confirm the deletion The wash protocol has now been deleted ...

Page 111: ...immersed in wash buffer or deionized distilled water Refer to Soak wash head on page 92 and Move wash head on page 93 Caution If the wash heads are not rinsed the tips may become clogged If this occurs the wash heads will need to be cleaned repaired or may have to be replaced 4 If the instrument will be left to stand for a longer time priming must be performed to remove all liquid from the system ...

Page 112: ...nual Thermo Fisher Scientific Warning When handling the waste bottle it is advisable to observe applicable safety precautions such as the wearing of disposable powder free gloves safety glasses and protective clothing to avoid potential infectious disease contamination ...

Page 113: ...instrument 1 Switch off the instrument see Figure 3 26 on page 34 2 Unplug the instrument immediately from the power supply see Figure 3 25 on page 34 3 Carry out appropriate corrective measures Do not disassemble the instrument 4 If the corrective measures taken do not help contact authorized technical service or your local Thermo Fisher Scientific representative Handling abnormal situations ...

Page 114: ...Emergency Situations Handling abnormal situations 112 Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific ...

Page 115: ...ces have been contaminated with biohazardous material disinfect with a mild sterilizing solution See Instrument care on page 114 Clean the case of the instrument periodically See Cleaning the instrument on page 115 Clean the plate carrier if needed See Cleaning the plate carrier on page 116 Clean the priming vessel if needed See Cleaning the priming vessel on page 115 Clean the liquid bottles regu...

Page 116: ...ellwash Versa Prime all the channels of the instrument with deionized distilled water after use Prevent any liquid from entering the instrument Keep the instrument free of dust and other foreign matter Perform operational checks regularly see Performing the operational check after switching on on page 31 In the event of any damage contact your local Thermo Fisher Scientific representative for serv...

Page 117: ...damage may occur 1 Switch the power off and unplug the instrument 2 Use disposable powder free gloves 3 Clean the instrument exterior and the plate carrier with a soft cloth dampened with water or mild detergent 4 If you have spilled infectious agents on the instrument decontaminate the instrument Refer to Decontamination procedure on page 122 Caution Do not use solutions containing hypochlorite s...

Page 118: ...d detergent and rinse with deionized distilled water 4 Replace the priming vessel by inserting it downwards and pulling it towards yourself Warning The priming vessel may be contaminated after the instrument has been used To clean the plate carrier Caution Do not disassemble the plate carrier 1 Switch the power off and unplug the instrument 2 Use disposable powder free gloves 3 If spillages have o...

Page 119: ...late carrier back to its normal position once it has been cleaned Empty the waste bottle before cleaning it according to disposal regulations see Disposal of materials on page 122 The bottles must be cleaned regularly depending on the applications using a mild detergent The liquid bottles tubes and tube connectors can be autoclaved some five to ten times Figure 11 44 The liquid level sensors or th...

Page 120: ...onal check after switching on on page 31 You may autoclave that is sterilize materials with pressurized steam Autoclavable and un autoclavable parts of the washer unit are listed in Table 11 9 Refer also to Figure 11 44 The autoclaving conditions are as follows at 121 C with 1 bar underpressure for 20 minutes Table 11 9 Autoclavable and un autoclavable parts of the washer Autoclavable Un autoclava...

Page 121: ...ect the wash heads thoroughly before you remove them Warning Prime the instrument without liquid with air to empty all liquid from the system before removing the wash heads Warning When handling the waste bottle it is advisable to observe applicable safety precautions such as the wearing of disposable powder free gloves safety glasses and protective clothing to avoid potential infectious disease c...

Page 122: ...ash heads that have an identification label Check the liquid line for possible leaks Ensure that the silicone tubes are intact and there are no breaks or holes in them To ensure valve operation first prime the system then lift the buffer bottle about 20 cm and finally ensure that the tips are not dripping Check the residual volume by dispensing 200 μl of 0 02 Tween solution over the whole plate As...

Page 123: ...hout a plate The wash head moves to the 0 0 mm level Ensure that the wash head tips are on the plate bottom level of the plate carrier Refer to Figure 6 40 Enable the Plate sensor after the test If both the Prime and Boost prime functions fail you have to perform manual priming To prime a dry pump manually Figure 11 46 1 Fill the buffer bottle completely with liquid and close the cap Place the bot...

Page 124: ...local regulations for the disposal of infectious material Warning The samples can be potentially infectious Dispose of all used plates disposable gloves syringes disposable tips and so on as biohazardous waste Be cautious and always use disposable gloves Warning Treat the used microplate waste bottle priming vessel on the plate carrier disposables and all substances used in accordance with good la...

Page 125: ...dehyde solution 4 Chloramine T Microcide SQ 1 64 Decon 90 min 4 Caution If local or laboratory regulations prescribe regular decontamination it is not advisable to use formaldehyde since even small traces of formaldehyde negatively affect the enzyme being used in EIA tests resulting in inconsistent test results 1 Prepare the decontaminant 2 Run the Maintenance Cleaning Disinfection procedure using...

Page 126: ... instrument using a mild detergent 12 After performing this decontamination procedure enclose a signed and dated Certificate of Decontamination see Appendix B both inside the transport package and attached to the outside of the package When you relocate the instrument or ship it for service make sure that you refit the transport lock 1 Switch off the instrument 2 Align the wash head arm and the pl...

Page 127: ...1 49 Transport lock and tag fitted A system log which includes a short summary of the use maintenance procedures error messages and other information about the use of the system aids proper maintenance of the system Refer to Appendix A System Log To pack the Wellwash Versa for service Provide information about the use of hazardous materials Prime all the channels of the instrument and clean the wa...

Page 128: ...emicals or radioactive chemicals waste management of the complete instrument must be carried out to ensure that there is no risk of contamination Warning Decontaminate the instrument before disposal Refer to Decontamination procedure on page 122 Caution Observe all federal state and local environmental regulations Follow laboratory and country specific procedures for biohazardous or radioactive wa...

Page 129: ...E symbol Do not treat electrical and electronic equipment as unsorted waste Collect waste from electrical and electronic equipment separately Regarding the original packaging and packing materials use the recycling operators known to you For more information contact your local Thermo Fisher Scientific representative ...

Page 130: ...Maintenance Disposal of the instrument 128 Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific ...

Page 131: ...ative humidity at 40 C Indoor use only Transportation conditions 40 C to 70 C packed in transport packaging Storage conditions 25 C to 50 C packed in transport packaging Mains power supply 100 240 Vac 50 60 Hz nominal Power consumption 100 VA max Heat dissipation 341 BTU max Noise 75 db at a distance of 500 mm Display High contrast color display with 480 x 272 dots Keypad Four arrow keys OK button...

Page 132: ...and with high aspirate speed at room temperature flat bottom 96 well plate Aspirate height Adjustable 0 14 mm in 0 1 mm increments Dispense volume 50 400 μl in 50 μl increments 96 well plate 20 120 μl in 10 μl increments 384 well plate Dispense accuracy 5 300 μl at room temperature with nx8 or nx12 wash head 5 250 μl with 2x8 cell wash head 5 100 μl with 1x16 wash head Dispense precision 3 CV 300 ...

Page 133: ...EC 60664 1 see Note 1 Pollution degree 2 according to IEC 60664 1 see Note 2 Note 1 The installation category overvoltage category defines the level of transient overvoltage which the instrument is designed to withstand safely It depends on the nature of the electricity supply and its overvoltage protection means For example in CAT II which is the category used for instruments in installations sup...

Page 134: ...Technical Specifications Safety specifications 132 Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific ...

Page 135: ...error Check the head movement Contact authorized technical service 6 Dispense pump rotation error The pump is jammed Contact authorized technical service 7 Dispense pump time out The pump is jammed Contact authorized technical service 8 Plate was not detected Insert the plate properly 9 Plate sensor doesn t work Contact authorized technical service 10 Non volatile parameters lost Contact authorize...

Page 136: ... Not enough memory Delete unused protocols 71 Adding the step not successful 80 Invalid step parameter s 81 Too many step parameters 82 At least one of the step parameters is missing 83 Step list is not empty 84 Creating a new step list failed 85 No strips are selected Select strips 86 Invalid SOF parameter 91 USB device timeout Try with another USB memory device Table 13 13 Warning codes reported...

Page 137: ...ur contact authorized technical service immediately Table 13 14 Troubleshooting guide Symptom Description Suggested action Dispensing volume is too low or residual volume is too high Tubing is old or clogged Wash head tips are blocked Too high an aspiration position Use Maintenance Clean Soak wash head Clean or replace the tubing Clean the wash head tips with the declogging tool Replace the wash h...

Page 138: ...correct Check the layout and place the plate in the correct orientation required by the wash head configuration Check the wash head and replace the correct wash head required by the layout and the protocol Liquid is spilled during operation Well volume or shake speed is too high Check the tubing and O rings and replace if needed Decrease the well volume or shake speed Priming failed Tubing incorre...

Page 139: ...h head 1 N10803 1x16 wash head Wellwash Versa 1 N10802 2x8 cell wash head Wellwash Versa 1 N10804 Blank wash head Wellwash Versa 1 N10807 2 liter wash bottle A Wellwash Versa 1 N10808 2 liter wash bottle B Wellwash Versa 1 N10809 2 liter rinse bottle Wellwash Versa 1 N10810 4 liter waste bottle square 1 N10811 4 liter wash bottle A round 1 N10812 4 liter wash bottle B round Wellwash Versa 1 N10813...

Page 140: ...ories Kit Incl declogging tools normal thin syringe 20 ml and silicon grease for O rings 1 N12409 Wash head tubing connector Kit 2 pcs each Incl dispensing and aspiration tubing connectors see Figure 14 50 B 1 N12408 Cell wash head adapter 2 pcs see Figure 14 50 A 1 N12407 Liquid filter 5 pcs 1 2305290 Serial cable F9 F25 1 SP 00250 PM Kit Wellwash Versa 1 Longer USB cables available from PC store...

Page 141: ...Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual 139 Appendix A System Log Instrument name number User Date Comments ...

Page 142: ...System Log 140 Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific ...

Page 143: ...have been decontaminated and can be handled without exposing the personnel to health hazards Materials used in the unit Chemicals Biological Radioactive Specific information on contaminants Decontamination procedure1 Date and place Signature Name block capitals The signature of a Radiation Safety Officer is also required when the unit has been used with radioactive materials This unit is certified...

Page 144: ...Certificate of Decontamination 142 Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific ...

Page 145: ...ing results A diagnostic test method to measure or detect a substance using antibody antigen reactions ELISA Abbreviation for enzyme linked immunosorbent assay remote control Running mode allowing a remote computer to operate the washer wash head Interchangeable twin strip 2x8 way 2x12 way or 1x blanking heads allowing either 1x8 2x8 1x12 or 2x12 processing A single 1x16 way wash head allows proce...

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Page 147: ...N11166 Thermo Fisher Scientific Oy Ratastie 2 P O Box 100 FI 01621 Vantaa Finland www thermoscientific com ...

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